Mitochondrial Functions and Cell Biology Quiz
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Questions and Answers

What occurs during the G1 phase of interphase?

  • Synthesis of macromolecules essential for DNA duplication begins. (correct)
  • DNA duplication takes place.
  • The cell divides its nucleus and cytoplasm.
  • Preparation for mitosis begins.
  • Which of the following statements is true regarding somatic cells?

  • They undergo meiosis for reproduction.
  • They do not utilize mitosis at any point.
  • They use mitosis for cell division. (correct)
  • They are always in a stable phase.
  • What is the function of nucleostemin in relation to cellular components?

  • It coexists with granular components but is unrelated to ribosomal biogenesis. (correct)
  • It aids in ribosomal biogenesis.
  • It exclusively functions in the G0 phase.
  • It cooperates with cytoskeletal elements.
  • During which phase does the replication of chromatin into discrete chromosomes occur?

    <p>Prophase (B)</p> Signup and view all the answers

    Which type of cells are said to be in a terminally differentiated G0 state?

    <p>Neurons and skeletal muscle cells (B)</p> Signup and view all the answers

    What structures in the mitochondria are responsible for increasing its surface area?

    <p>Cristae (A)</p> Signup and view all the answers

    Which enzyme complex allows protons to flow back into the mitochondrial matrix?

    <p>ATP synthase (D)</p> Signup and view all the answers

    What is a major source of electrons for the electron-transport chain?

    <p>NADH (B)</p> Signup and view all the answers

    What evidence supports the endosymbiotic theory regarding mitochondria?

    <p>Mitochondria have their own round DNA. (C)</p> Signup and view all the answers

    What is formed as a byproduct of fatty acid catabolism in peroxisomes?

    <p>Hydrogen peroxide (A)</p> Signup and view all the answers

    What molecule is primarily converted to acetyl CoA in mitochondria during the Krebs cycle?

    <p>Pyruvate (D)</p> Signup and view all the answers

    Which process is not performed by peroxisomes?

    <p>Production of ATP (D)</p> Signup and view all the answers

    What is the function of the electrochemical gradient in mitochondria?

    <p>To promote ATP synthesis (C)</p> Signup and view all the answers

    What are the components of a prokaryotic ribosome?

    <p>50S subunit and 30S subunit (D)</p> Signup and view all the answers

    Which protein is responsible for transporting vesicles towards the positive end of the cytoskeleton?

    <p>Kinesin (B)</p> Signup and view all the answers

    How does the stiffness of the substrate affect cell differentiation?

    <p>Softer substrates promote the expression of brain cell genes. (C)</p> Signup and view all the answers

    What is the role of blebbistatin in cell differentiation studies?

    <p>Blocks cell contraction, preventing matrix assessment. (B)</p> Signup and view all the answers

    Integrins are primarily involved in which function within the cell?

    <p>Cell adhesion and substrate binding (B)</p> Signup and view all the answers

    What are the three approaches to tissue engineering?

    <p>Using a matrix, drugs, and cell implantation (D)</p> Signup and view all the answers

    What distinguishes eukaryotic ribosomes from prokaryotic ribosomes?

    <p>Eukaryotic ribosomes have different subunit sizes. (B)</p> Signup and view all the answers

    Which cell shape is associated with the differentiation into osteoblasts?

    <p>Star shape (A)</p> Signup and view all the answers

    What is the primary function of the Golgi apparatus?

    <p>Post translational modification of proteins (D)</p> Signup and view all the answers

    Where does the packing of RER content into vesicles primarily occur?

    <p>Golgi apparatus (D)</p> Signup and view all the answers

    What is the role of lysosomes in cellular function?

    <p>Digest bacteria and cellular debris (A)</p> Signup and view all the answers

    What characteristic of epithelial cells specialized for secretion is noted in their polarity?

    <p>RER is abundant at the basal ends (A)</p> Signup and view all the answers

    Which compartment is located between RER and the cis-face of the Golgi apparatus?

    <p>Vesicular-tubular clusters (VTCs) (D)</p> Signup and view all the answers

    What is a key function of mitochondria in cells?

    <p>Act as sensors of cellular health (C)</p> Signup and view all the answers

    What type of environment is required for lysosomal enzymes to be active?

    <p>Acidic pH (C)</p> Signup and view all the answers

    Which of the following does not define the structure of the Golgi apparatus?

    <p>Presence of ribosomes (C)</p> Signup and view all the answers

    Which process involves the cell membrane engulfing particles like bacteria?

    <p>Phagocytosis (B)</p> Signup and view all the answers

    What is the primary function of coated vesicles in endocytosis?

    <p>To mediate the internalization of ligands when receptors are bound (D)</p> Signup and view all the answers

    How does receptor-mediated endocytosis differ from regular endocytosis?

    <p>It requires specific binding of receptors to ligands. (C)</p> Signup and view all the answers

    What happens to the vesicle after exocytosis takes place?

    <p>It releases materials outside the cell and adds to the cell membrane. (A)</p> Signup and view all the answers

    What is the initial action of the cell membrane during pinocytosis?

    <p>Invaginating to form a pit. (A)</p> Signup and view all the answers

    During cell signaling, what role does the vesicle play when it contains the ligand-receptor complex?

    <p>It continues to emit signals within the cell. (A)</p> Signup and view all the answers

    Which of the following best describes phagocytosis?

    <p>The engulfing of large particles. (B)</p> Signup and view all the answers

    What happens to the cell membrane during the receptor-mediated endocytosis process?

    <p>It forms a new membrane component after vesicle fusion. (D)</p> Signup and view all the answers

    What role does the rough endoplasmic reticulum (RER) primarily serve in the cell?

    <p>Production and secretion of proteins (C)</p> Signup and view all the answers

    Which of the following correctly describes the smooth endoplasmic reticulum (SER)?

    <p>It plays a key role in the synthesis of cholesterol. (A)</p> Signup and view all the answers

    How do ribosomes aid in the process of protein synthesis?

    <p>They move along the mRNA to decode genetic information. (C)</p> Signup and view all the answers

    What function does the endoplasmic reticulum lumen serve in the cell?

    <p>Storage of lipid droplets (A)</p> Signup and view all the answers

    In which type of cells is the smooth endoplasmic reticulum (SER) particularly well-developed for muscle contraction?

    <p>Striated muscle cells (C)</p> Signup and view all the answers

    What type of cells typically have a high presence of free ribosomes in the cytoplasm?

    <p>Erythroblasts producing hemoglobin (A)</p> Signup and view all the answers

    What is one of the key roles of the endoplasmic reticulum in relation to mitochondria?

    <p>To synthesize phospholipids (A)</p> Signup and view all the answers

    Which statement correctly describes the staining characteristic of the cytoplasm in cells with abundant RER?

    <p>It stains pink because it is eosinophilic. (C)</p> Signup and view all the answers

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    What is the primary function of phagocytosis?

    <p>To internalize large particles such as bacteria (A)</p> Signup and view all the answers

    Which process involves the invagination of the cell membrane to form a vesicle containing extracellular fluid?

    <p>Pinocytosis (B)</p> Signup and view all the answers

    What activates the formation of coated vesicles during receptor-mediated endocytosis?

    <p>The binding of the ligand to its receptor (C)</p> Signup and view all the answers

    During which process does the cell membrane fuse with a vesicle to release its contents outside the cell?

    <p>Exocytosis (C)</p> Signup and view all the answers

    Which type of vesicle is typically involved in the signaling process inside a cell after endocytosis?

    <p>Endocytic vesicle (A)</p> Signup and view all the answers

    How do receptor-mediated endocytosis and regular endocytosis differ?

    <p>Receptor-mediated endocytosis uses ligands for internalization, while regular does not (C)</p> Signup and view all the answers

    Which protein is commonly associated with coated vesicles involved in the endocytic process?

    <p>Clathrin (D)</p> Signup and view all the answers

    What occurs to the ligands after they are internalized in receptor-mediated endocytosis?

    <p>They continuously bind to their receptors inside the vesicle (B)</p> Signup and view all the answers

    What distinguishes the rough endoplasmic reticulum (RER) from the smooth endoplasmic reticulum (SER)?

    <p>RER has ribosomes on its surface, while SER does not. (C)</p> Signup and view all the answers

    Which subunit of the ribosome is responsible for the exit of the polypeptide chain during translation?

    <p>E site (A)</p> Signup and view all the answers

    What role does the smooth endoplasmic reticulum (SER) play in muscle cells?

    <p>Calcium sequestration and release. (B)</p> Signup and view all the answers

    How does the rough endoplasmic reticulum (RER) differ in cells that primarily produce hemoglobin?

    <p>It has a high amount of ribosomes. (A)</p> Signup and view all the answers

    What is the primary function of the endoplasmic reticulum lumen?

    <p>Lipid droplet storage. (B)</p> Signup and view all the answers

    Which enzyme is involved in the detoxification of harmful exogenous molecules in the smooth endoplasmic reticulum?

    <p>Cytochrome P450. (C)</p> Signup and view all the answers

    What characterizes the appearance of the cytoplasm in cells abundant with rough endoplasmic reticulum when stained with H&E?

    <p>It turns pink, reflecting eosinophilia. (B)</p> Signup and view all the answers

    In which type of cell is the smooth endoplasmic reticulum (SER) especially well developed for its role in contraction?

    <p>Striated muscle cell. (B)</p> Signup and view all the answers

    What is the primary function of lipid droplets in cells?

    <p>They store lipids and actively transport them within the cell. (C)</p> Signup and view all the answers

    What is true about glycogen granules?

    <p>They are aggregates of glycogen used as a quick energy source. (A)</p> Signup and view all the answers

    What is the condition called when a cell has one set of chromosomes?

    <p>Haploid (B)</p> Signup and view all the answers

    Which statement accurately describes necrosis?

    <p>The cell's plasma membrane bursts, releasing internal molecules. (C)</p> Signup and view all the answers

    Which function best describes the role of exportins?

    <p>They export macromolecules from the nucleus to the cytoplasm. (C)</p> Signup and view all the answers

    What aspect distinguishes sex chromosomes from autosomes?

    <p>Their role in determining biological sex (D)</p> Signup and view all the answers

    Which of the following accurately describes a Barr body?

    <p>A silenced X chromosome in females (D)</p> Signup and view all the answers

    What is one of the controlling mechanisms of apoptosis?

    <p>Release of caspase-activating enzymes. (A)</p> Signup and view all the answers

    Which type of epithelial tissue is responsible for forming glands that secrete substances?

    <p>Secretory/glandular epithelia (C)</p> Signup and view all the answers

    What is the significance of pigment deposits like melanin granules in cells?

    <p>They protect cell nuclei from DNA damage caused by light. (A)</p> Signup and view all the answers

    What is the main function of the nucleolus within the cell's nucleus?

    <p>To produce and assemble ribosomes (A)</p> Signup and view all the answers

    What is a primary characteristic of epithelial tissue compared to connective tissue?

    <p>Epithelial cells have sides and are polarized. (B)</p> Signup and view all the answers

    How many pairs of autosomes does a normal human karyotype have?

    <p>22 (A)</p> Signup and view all the answers

    What is the primary structure surrounding the nucleus?

    <p>Nuclear envelope composed of two membranes. (C)</p> Signup and view all the answers

    What is the role of the basement membrane for epithelial cells?

    <p>It connects epithelium to connective tissue. (D)</p> Signup and view all the answers

    Which component facilitates the bidirectional traffic between the nucleus and the cytoplasm?

    <p>Nuclear localization signals and transport proteins. (A)</p> Signup and view all the answers

    Which component of the basement membrane allows epithelial cells to connect to their environment?

    <p>Laminin (C)</p> Signup and view all the answers

    What happens to telomeres with each cell division?

    <p>They shorten indicating the cell is aging (B)</p> Signup and view all the answers

    What is the primary function of the apical domain of epithelial cells?

    <p>Displays specialized features to interact with the lumen or external environment (D)</p> Signup and view all the answers

    What term describes the display of an organism's complete set of chromosomes?

    <p>Karyotype (C)</p> Signup and view all the answers

    Which component of the nucleolus is primarily involved in the assembly of ribosomal subunits?

    <p>Granular component (C)</p> Signup and view all the answers

    In contrast to epithelial tissue, which type of tissue does not have polarized cells?

    <p>Connective tissue (D)</p> Signup and view all the answers

    Which connective tissue component is essential for the mechanical stability of epithelial tissues?

    <p>Basement membrane (B)</p> Signup and view all the answers

    What is the primary function of connective tissue in relation to other tissues?

    <p>To provide support and protection (D)</p> Signup and view all the answers

    During which phase do sister chromatids separate and move towards opposite spindle poles?

    <p>Anaphase (C)</p> Signup and view all the answers

    What primarily causes the asymmetrical division of stem cells?

    <p>Extracellular environment (B)</p> Signup and view all the answers

    What structural component forms a cleavage furrow during cytokinesis?

    <p>Actin filaments and myosin (A)</p> Signup and view all the answers

    What do kinetochores attach to during metaphase?

    <p>Centromere DNA (C)</p> Signup and view all the answers

    What is the primary role of stem cells in tissues?

    <p>To differentiate into specialized cells (D)</p> Signup and view all the answers

    What occurs to the nuclear envelope during telophase?

    <p>It reassembles around daughter chromosomes (A)</p> Signup and view all the answers

    What describes the primary difference between stem cells and progenitor cells?

    <p>Stem cells can divide indefinitely, progenitor cells cannot. (A)</p> Signup and view all the answers

    Which phase of mitosis is characterized by the further condensation of chromosomes?

    <p>Metaphase (C)</p> Signup and view all the answers

    Flashcards

    Endocytosis

    The process of bringing substances from outside the cell into the cell's interior.

    Phagocytosis

    A type of endocytosis where the cell engulfs large particles, like bacteria, into a phagosome.

    Pinocytosis

    A type of endocytosis where the cell takes in small droplets of extracellular fluid into a vesicle.

    Receptor-mediated endocytosis

    A type of endocytosis where specific molecules bind to receptors on the cell membrane and are taken into the cell in a vesicle.

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    Exocytosis

    The process of releasing substances from inside the cell to the outside.

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    Coated Vesicles

    Vesicles that are covered in special proteins, like clathrin, which help them form and function.

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    Clathrin

    Special proteins, like clathrin, that surround and shape vesicles.

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    Cell Signaling Through Vesicles

    A signaling pathway where the ligand-receptor complex is endocytosed, creating a signal-emitting vesicle within the cell.

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    Motor proteins

    Large protein complexes that help transport vesicles along the cytoskeleton.

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    Integrins

    Proteins that bind to the substrate and form focal adhesions.

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    Intermediate filaments

    A network of protein filaments that provides structural support to the cell, keeping organelles in place.

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    Mechanotransduction

    The process of cell differentiation into different cell types based on the stiffness of the surrounding environment.

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    Tissue engineering

    The process of creating new tissues to replace damaged or lost ones.

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    30S subunit

    The smallest unit of ribosomes found in prokaryotes.

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    60S subunit

    The larger subunit of eukaryotic ribosomes.

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    rRNA

    A type of RNA that has enzymatic activity during protein synthesis.

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    Mitosis

    The time period when the cell divides its nucleus and cytoplasm, resulting in two daughter cells.

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    Interphase

    The time period when the cell grows, duplicates its genetic material, and prepares for division.

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    G0 Phase

    A stage where cells that have exited the cell cycle remain inactive, similar to sleeping.

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    DNA Replication

    The process of duplicating DNA to create two copies of each chromosome, ensuring each daughter cell receives a complete set.

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    Prophase

    The first phase of mitosis where the nucleolus disappears, chromosomes condense, and microtubules form a spindle to separate the chromosomes.

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    Mitochondrial Cristae

    A highly folded inner membrane of mitochondria, increasing surface area for ATP production.

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    Mitochondrial Matrix

    The gel-like substance inside mitochondria, containing enzymes crucial for ATP production.

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    Endosymbiotic Theory

    The theory explaining the origin of mitochondria, stating they were once free-living bacteria.

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    Krebs Cycle in Mitochondria

    The process by which pyruvate and fatty acids are converted to acetyl CoA, yielding ATP and NADH, a key electron carrier.

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    Electron Transport Chain (ETC)

    The movement of electrons through protein complexes in the inner mitochondrial membrane, generating a proton gradient.

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    ATP Synthase

    An enzyme complex in mitochondria that uses the proton gradient to synthesize ATP.

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    Peroxisomes

    Small, membrane-bound organelles in the cytoplasm containing oxidative enzymes.

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    Beta Oxidation

    The process of breaking down long-chain fatty acids in peroxisomes, generating acetyl CoA and hydrogen peroxide.

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    Secretory Epithelial Cells

    Specialized epithelial cells that secrete substances into an enclosed lumen, like pancreatic acinar cells releasing digestive enzymes into the intestine.

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    Golgi Apparatus

    A cellular organelle composed of stacked, flattened membrane-bound sacs called cisternae, involved in protein modification, sorting, and packaging.

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    Cis-Face of Golgi

    The receiving end of the Golgi apparatus, closest to the RER, where proteins enter the Golgi.

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    Trans-Face of Golgi

    The releasing end of the Golgi apparatus, where modified proteins are packaged into vesicles and sent to their final destinations.

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    Lysosomes

    Membrane-bound organelles containing hydrolytic enzymes, responsible for breaking down cellular waste, worn-out organelles, and ingested materials.

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    Mitochondria

    The powerhouse of the cell, responsible for producing ATP, the main energy currency used in cellular processes.

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    Outer Membrane of Mitochondria

    The outer membrane of the mitochondria, containing porins, which allow small molecules to pass through.

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    Apoptosis

    The process of programmed cell death, initiated by mitochondria in response to cellular stress.

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    What are ribosomes and what is their function?

    Ribosomes are essential for protein synthesis. They are composed of two subunits: a large and a small subunit. The subunits are assembled within the nucleolus, and then exported to the cytoplasm. Once in the cytoplasm, ribosomes bind to mRNA and move along it to read the genetic code contained within the mRNA sequence.

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    Describe the three sites of a ribosome.

    The 'A' site is the aminoacyl site where a new tRNA carrying an amino acid binds to the mRNA. The 'P' site is the peptidyl site where the growing polypeptide chain is attached to the tRNA. The 'E' site is the exit site where the tRNA, which has released its amino acid, leaves the ribosome.

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    What is the endoplasmic reticulum and what are its main types?

    The endoplasmic reticulum (ER) is a network of interconnected membranes that extends throughout the cytoplasm of eukaryotic cells. It is a vast organelle that plays several vital roles in the cell, including protein synthesis, lipid metabolism, and detoxification. The ER is divided into two main regions: the rough ER (RER) and the smooth ER (SER).

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    What is the rough endoplasmic reticulum (RER) and what are its functions?

    The RER is studded with ribosomes, which are responsible for protein synthesis and translation. These ribosomes give the RER its 'rough' appearance. The RER is primarily involved in producing proteins that are destined for secretion outside of the cell or for incorporation into membranes. The flattened sacs of the RER are called cisternae.

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    What is the smooth endoplasmic reticulum (SER) and what are its main roles?

    The SER lacks ribosomes, giving it a smooth appearance. Its functions are diverse and include: 1) Synthesis of lipids (e.g., phospholipids, steroids), 2) Detoxification of harmful substances (e.g., drugs, alcohol), 3) Calcium storage and release. This plays a key role in muscle contraction. 4) Lipid droplet biogenesis.

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    What is the ER lumen?

    The lumen of the ER is the internal space enclosed by the ER membrane. It plays an important role in protein folding and modification. It is also the site of lipid droplet biogenesis.

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    Explain the relationship between RER and secretory activities of cells.

    Cells that produce few or no proteins for secretion, like erythrocytes, have little RER and primarily free ribosomes in their cytoplasm. Cells that synthesize, segregate, and store proteins for secretion, like eosinophilic leucocytes, have well-developed RER, Golgi apparatus, and secretory granules.

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    Explain the role of SER in calcium regulation.

    The SER plays a crucial role in calcium sequestration and controlled release. This is particularly important in striated muscle cells where the SER, specialized as the sarcoplasmic reticulum, regulates muscle contraction.

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    What are lipid droplets?

    Lipid droplets are single-membrane vesicles that store lipids. They are abundant in cells of the adrenal cortex and cooperate with other organelles to transport lipids effectively.

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    What are glycogen granules?

    Glycogen granules are clusters of glycogen, a carbohydrate polymer, used to store glucose. They lack a membrane and are abundant in cells with high energy demands.

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    What are pigment deposits?

    Pigment deposits (PD) are cellular structures that contain diverse complex substances like melanin granules. Melanin protects cell nuclei from light damage.

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    What is necrosis?

    Necrosis is a form of cell death where the cell swells, its membrane ruptures, and contents leak out, causing inflammation.

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    What is apoptosis?

    Apoptosis is programmed cell death where the cell breaks down into small pieces without causing inflammation. It is regulated by molecules like cytochrome C.

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    What is the nuclear envelope?

    The nuclear envelope is a double-membrane structure surrounding the nucleus. It's connected to the ER and regulates the passage of molecules.

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    What are importins and exportins?

    Importins transport molecules from the cytoplasm into the nucleus, while exportins transport molecules out of the nucleus into the cytoplasm.

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    What is the perinuclear cisterna?

    The perinuclear cisterna is the space between the two membranes of the nuclear envelope. It's connected to the ER and allows for communication between these structures.

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    Sex chromosomes vs. Autosomes

    X and Y chromosomes are the sex chromosomes, while the remaining 22 pairs in the karyotype are called autosomes.

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    Haploid vs. Diploid

    Haploid cells have one set of chromosomes (n), while diploid cells have two sets (2n). This refers to the total number of chromosomes present in a cell.

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    What is a karyotype?

    A karyotype is an organized display of an individual's chromosomes. A normal human karyotype contains 46 chromosomes arranged in 23 pairs.

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    What are FISH probes?

    FISH probes bind to specific DNA sequences, allowing scientists to identify and visualize different regions of chromosomes.

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    What is a Barr body?

    The Barr body is a condensed, inactive X chromosome found only in female cells. It's essentially a silenced X chromosome.

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    What are telomeres?

    Telomeres are protective caps at the ends of chromosomes that shorten with each cell division. This serves as a cellular aging clock.

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    What is the nucleolus?

    The nucleolus is a spherical structure inside the nucleus that manufactures ribosomes, essential for protein synthesis.

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    What are the components of the nucleolus?

    The nucleolus is divided into three components: the fibrillar center (rRNA genes and Pol I), the dense fibrillar component (nascent rRNA), and the granular component (ribosomal subunit assembly).

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    Describe the three sites of a ribosome: A, P, and E.

    The 'A' site: where a new tRNA carrying an amino acid binds to the mRNA. The 'P' site: where the growing polypeptide chain is attached to the tRNA. The 'E' site: where the tRNA that has released its amino acid exits the ribosome.

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    What is the endoplasmic reticulum and its main types?

    The ER is a network of interconnected membranes extending throughout the cytoplasm. It plays crucial roles in protein synthesis, lipid metabolism, and detoxification. It's of two main types: rough ER (RER) and smooth ER (SER).

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    What is the rough endoplasmic reticulum (RER) and its functions?

    The RER has ribosomes attached, giving it a rough appearance, and participates in producing proteins destined for secretion or membrane inclusion. Its flattened sacs are called cisternae.

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    What is the smooth endoplasmic reticulum (SER) and its main functions?

    The SER lacks ribosomes, making it smooth. It is involved in lipid synthesis (phospholipids, steroids), detoxification, calcium storage and release, and contributes to lipid droplet biogenesis.

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    Explain the relationship between RER and the secretory activities of cells.

    Cells with little RER and mostly free ribosomes produce few proteins for secretion (like erythrocytes), while those actively secreting (like eosinophilic leucocytes) have well-developed RER, Golgi, and secretory granules.

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    What happens during metaphase?

    The stage of mitosis where replicated chromosomes condense further, kinetochores attach to the spindle, and chromosomes align at the equatorial plate.

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    What happens during anaphase?

    The stage of mitosis where sister chromatids separate and move towards opposite poles of the cell due to microtubule motor proteins and dynamic changes in microtubule length.

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    What happens during telophase?

    The final stage of mitosis where chromosomes uncoil, the nuclear envelope reforms, and the spindle disappears.

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    What is cytokinesis?

    The process of dividing the cytoplasm and organelles into two daughter cells following mitosis. It involves a contractile ring of actin filaments that constricts and forms a cleavage furrow.

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    What is asymmetrical cell division?

    A type of cell division where the DNA is divided equally between two daughter cells, but the daughter cells may differ in their fate or function due to differences in the extracellular environment.

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    What are stem cells?

    Cells that have the potential to differentiate into various cell types and self-renew indefinitely.

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    What are tissues?

    Organized structures composed of cells and extracellular matrix. They are found in both embryonic and adult organisms. Examples include muscle tissue, nervous tissue, and connective tissue.

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    How does the extracellular environment affect stem cells?

    The extracellular environment can influence the fate of stem cells during asymmetrical cell division, leading to the differentiation of one daughter cell into a specific cell type while the other maintains its stem cell characteristics.

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    Epithelial Tissue

    A type of tissue that forms the outer layer of organs and lines body cavities. It consists of tightly packed cells with little extracellular matrix. Its main functions are protection, secretion, and absorption.

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    Secretory/Glandular Epithelia

    Epithelial cells that are specialized to release substances either externally, like sweat glands, or into internal cavities, like gastric glands.

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    Apical Domain

    The side of an epithelial cell that faces the lumen or external environment. It may have special features like cilia or microvilli.

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    Lateral Domain

    The side of an epithelial cell that interacts with neighboring cells. It's connected by cell adhesion molecules and junctional complexes.

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    Basal Domain

    The side of an epithelial cell that rests on the basement membrane. It gets nutrients and oxygen from the underlying connective tissue.

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    Basement Membrane

    A thin, specialized structure composed of proteins. It anchors epithelial tissue to the underlying connective tissue, providing mechanical support and regulating communication between the two.

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    Basal Lamina

    The inner layer of the basement membrane. It's rich in laminin and type IV collagen, providing structural support and regulating cell behavior.

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    Reticular Lamina

    The outer layer of the basement membrane. It contains collagen type III fibers and is connected to the basal lamina by anchoring fibrils of type VII collagen.

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    Study Notes

    Histology

    • Histology is the study of the microscopic structure of normal tissues, enabling understanding of tissue function.
    • Tissues are organized collections of cells with similar morphology (epithelial, muscular, nervous, connective).
    • Cell size ranges from 10-30 micrometers.
    • Microscopes are used to observe cells.

    Light Transmission Microscopy

    • Uses visible light passing through a sample.
    • Objective lenses magnify and project the image to the eyepiece.
    • Magnifications range from X40 to X400.
    • Samples must be transparent.
    • Tissue sections are prepared for viewing.

    Tissue Preparation

    • Fixation: Preserves tissue structure by cross-linking proteins and inactivating enzymes.
    • Dehydration: Removing water using increasingly concentrated alcohol solutions (65% to 100%).
    • Clearing: Replacing alcohol with an organic solvent (e.g., paraffin).
    • Infiltration: Embedding tissue in melted paraffin wax.
    • Embedding: Hardening the paraffin-infiltrated tissue in a mold.
    • Trimming: Preparing the paraffin block for sectioning on a microtome.

    Staining

    • Sections are stained to visualize structures and substances.
    • Hematoxylin and eosin (H&E) stain is commonly used.
    • Cell nuclei stain blue, while most cytoplasm stains pink/red.
    • PAS (periodic acid-Schiff) stains carbohydrates, either alone or with other molecules.

    Immunochemistry

    • Uses antibodies to target specific molecules (e.g., proteins) of interest.
    • Antibodies bind to target antigens, allowing identification & visualization.

    Microscopy Techniques

    • Phase-contrast microscopy: Useful for observing unstained specimens.
    • Confocal microscopy: Images one precise slice of the sample.
    • Stereomicroscopy: Used to observe 3D, non-transparent objects.
    • Super-resolution microscopy: STORM, STED, high-resolution images.

    Electron Microscopy

    • Transmission electron microscopy (TEM): 2D images of thin sections.
    • Scanning electron microscopy (SEM): 3D images of surfaces.

    The Cell

    • The plasma membrane is a double layer of phospholipids with cholesterol and proteins.
    • Cholesterol affects the fluidity and flexibility.
    • Proteins facilitate recognition, signaling, and transport across the membrane.
    • Glycosidic chains are present on phospholipids and proteins (glycolipids and glycoproteins).
    • Hyaluronic acid creates a jelly-like layer outside the cell, providing cushioning and lubrication.

    Membrane Transport

    • Simple diffusion: Lipophilic and small uncharged molecules cross membranes passively.
    • Channel/facilitated diffusion: Membrane proteins allow passage of specific chemicals.
    • Active transport (pumps): Moves molecules against concentration gradients.

    Cytoskeleton

    • Microtubules: Hollow tubes formed by tubulin; crucial for cell division, intracellular transport, and structure.
    • Microfilaments: Two strands of actin; important in cell movements, structure, and cell contraction.
    • Intermediate filaments: Structural support for cells.

    Organelles

    • Ribosomes: Sites of protein synthesis.
    • Endoplasmic reticulum (ER): Synthesis and modification of proteins; lipid metabolism.
    • Golgi apparatus: Processing, sorting, and modifying proteins and lipids.
    • Lysosomes: Contain enzymes for digestion/waste removal.
    • Mitochondria: Energy production (ATP) through cellular respiration.
    • Peroxisomes: Involved in oxidation reactions.

    Cellular Inclusion

    • Lipid droplets: Store lipids in cells.
    • Glycogen granules: Store glycogen (energy).
    • Pigment deposits: Protect cell nuclei (e.g., melanin).
    • Hemosiderin granules: Store iron.

    Cell Death

    • Necrosis: Cell swelling and rupture; causes inflammation.
    • Apoptosis: Programmed cell death, not inflammatory.

    The Nucleus

    • The nucleus is a double membrane-bound structure containing the cell's DNA.
    • Pores regulate traffic between the nucleus and the cytoplasm.
    • Chromatin (DNA complexed with histones) is organized into nucleosomes.
    • Chromosomes condense during cell division.
    • The nucleolus is the site of ribosome assembly.

    Cell Cycle

    • The cell cycle consists of interphase (G1, S, G2) and the mitotic phase (mitosis and cytokinesis).
    • Cell cycle checkpoints are critical for regulated cell growth and division.

    Tissues

    • Tissues are organized groups of similar cells working together.
    • Epithelial tissues: Cover body surfaces, line cavities, and form glands.
    • Connective tissues: Provide support, structure, and connect tissues.
    • Muscle tissues: Responsible for movement.
    • Nervous tissues: Transmit electrical signals.

    Types of Epithelia

    • Covering/lining epithelia: Classified by cell shape (squamous, cuboidal, columnar) and layering (simple, stratified).
    • Glandular epithelia: Specialized for secretion; unicellular (e.g., goblet cells) or multicellular (e.g., salivary glands, pancreas).

    Specializations of Epithelial Cells

    • Microvilli: Increase surface area for absorption.
    • Stereocilia: Increase surface area (e.g., in the inner ear).
    • Cilia: Movement of substances (e.g., in the respiratory tract).
    • Cell-to-cell junctions (tight junctions, adherens junctions, desmosomes, gap junctions): attach, communicate, or block substances from passing between cells.

    Connective Tissues

    • Connective tissues have a matrix that contains ground substance (GAGs, proteoglycans, proteins) and fibers (collagen, elastic).
    • Loose connective tissues: Support and connect tissues (e.g., lamina propria).
    • Dense connective tissues: Support and provide strength and structure (e.g., tendons, ligaments).
    • Cartilage: Tough, flexible support (hyaline, elastic, fibrocartilage).
    • Bone: Hard, mineralized support (spongy, compact).

    Specialised Cells

    • Fibroblast: Maintain and synthesize collagen fibers.
    • Macrophages: Phagocytic cells.
    • Mast cells: Release histamine/heparin in inflammation.
    • Lymphocytes(B-lymphocytes and T-lymphocytes): part of the immune system.
    • Plasma cells: Antibody production.
    • Osteoblasts: Produce bone matrix.
    • Osteocytes: Maintain bone matrix.
    • Osteoclasts: Resorb bone matrix.

    Bone Remodeling

    • A cyclical process of bone resorption and formation, important for maintaining bone calcium levels and adapting to stress.
    • Involved in many physiological processes including fracture healing.

    Types of Gland

    • Exocrine Glands: Secrete products into ducts that empty onto epithelial surfaces (e.g., Sweat glands, salivary glands, goblet cells).
    • Endocrine Glands: Secrete hormones directly into blood (e.g., Thyroid gland, adrenal glands, pancreas).

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