Podcast
Questions and Answers
What occurs during the G1 phase of interphase?
What occurs during the G1 phase of interphase?
Which of the following statements is true regarding somatic cells?
Which of the following statements is true regarding somatic cells?
What is the function of nucleostemin in relation to cellular components?
What is the function of nucleostemin in relation to cellular components?
During which phase does the replication of chromatin into discrete chromosomes occur?
During which phase does the replication of chromatin into discrete chromosomes occur?
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Which type of cells are said to be in a terminally differentiated G0 state?
Which type of cells are said to be in a terminally differentiated G0 state?
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What structures in the mitochondria are responsible for increasing its surface area?
What structures in the mitochondria are responsible for increasing its surface area?
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Which enzyme complex allows protons to flow back into the mitochondrial matrix?
Which enzyme complex allows protons to flow back into the mitochondrial matrix?
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What is a major source of electrons for the electron-transport chain?
What is a major source of electrons for the electron-transport chain?
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What evidence supports the endosymbiotic theory regarding mitochondria?
What evidence supports the endosymbiotic theory regarding mitochondria?
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What is formed as a byproduct of fatty acid catabolism in peroxisomes?
What is formed as a byproduct of fatty acid catabolism in peroxisomes?
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What molecule is primarily converted to acetyl CoA in mitochondria during the Krebs cycle?
What molecule is primarily converted to acetyl CoA in mitochondria during the Krebs cycle?
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Which process is not performed by peroxisomes?
Which process is not performed by peroxisomes?
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What is the function of the electrochemical gradient in mitochondria?
What is the function of the electrochemical gradient in mitochondria?
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What are the components of a prokaryotic ribosome?
What are the components of a prokaryotic ribosome?
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Which protein is responsible for transporting vesicles towards the positive end of the cytoskeleton?
Which protein is responsible for transporting vesicles towards the positive end of the cytoskeleton?
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How does the stiffness of the substrate affect cell differentiation?
How does the stiffness of the substrate affect cell differentiation?
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What is the role of blebbistatin in cell differentiation studies?
What is the role of blebbistatin in cell differentiation studies?
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Integrins are primarily involved in which function within the cell?
Integrins are primarily involved in which function within the cell?
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What are the three approaches to tissue engineering?
What are the three approaches to tissue engineering?
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What distinguishes eukaryotic ribosomes from prokaryotic ribosomes?
What distinguishes eukaryotic ribosomes from prokaryotic ribosomes?
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Which cell shape is associated with the differentiation into osteoblasts?
Which cell shape is associated with the differentiation into osteoblasts?
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What is the primary function of the Golgi apparatus?
What is the primary function of the Golgi apparatus?
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Where does the packing of RER content into vesicles primarily occur?
Where does the packing of RER content into vesicles primarily occur?
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What is the role of lysosomes in cellular function?
What is the role of lysosomes in cellular function?
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What characteristic of epithelial cells specialized for secretion is noted in their polarity?
What characteristic of epithelial cells specialized for secretion is noted in their polarity?
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Which compartment is located between RER and the cis-face of the Golgi apparatus?
Which compartment is located between RER and the cis-face of the Golgi apparatus?
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What is a key function of mitochondria in cells?
What is a key function of mitochondria in cells?
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What type of environment is required for lysosomal enzymes to be active?
What type of environment is required for lysosomal enzymes to be active?
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Which of the following does not define the structure of the Golgi apparatus?
Which of the following does not define the structure of the Golgi apparatus?
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Which process involves the cell membrane engulfing particles like bacteria?
Which process involves the cell membrane engulfing particles like bacteria?
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What is the primary function of coated vesicles in endocytosis?
What is the primary function of coated vesicles in endocytosis?
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How does receptor-mediated endocytosis differ from regular endocytosis?
How does receptor-mediated endocytosis differ from regular endocytosis?
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What happens to the vesicle after exocytosis takes place?
What happens to the vesicle after exocytosis takes place?
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What is the initial action of the cell membrane during pinocytosis?
What is the initial action of the cell membrane during pinocytosis?
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During cell signaling, what role does the vesicle play when it contains the ligand-receptor complex?
During cell signaling, what role does the vesicle play when it contains the ligand-receptor complex?
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Which of the following best describes phagocytosis?
Which of the following best describes phagocytosis?
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What happens to the cell membrane during the receptor-mediated endocytosis process?
What happens to the cell membrane during the receptor-mediated endocytosis process?
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What role does the rough endoplasmic reticulum (RER) primarily serve in the cell?
What role does the rough endoplasmic reticulum (RER) primarily serve in the cell?
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Which of the following correctly describes the smooth endoplasmic reticulum (SER)?
Which of the following correctly describes the smooth endoplasmic reticulum (SER)?
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How do ribosomes aid in the process of protein synthesis?
How do ribosomes aid in the process of protein synthesis?
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What function does the endoplasmic reticulum lumen serve in the cell?
What function does the endoplasmic reticulum lumen serve in the cell?
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In which type of cells is the smooth endoplasmic reticulum (SER) particularly well-developed for muscle contraction?
In which type of cells is the smooth endoplasmic reticulum (SER) particularly well-developed for muscle contraction?
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What type of cells typically have a high presence of free ribosomes in the cytoplasm?
What type of cells typically have a high presence of free ribosomes in the cytoplasm?
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What is one of the key roles of the endoplasmic reticulum in relation to mitochondria?
What is one of the key roles of the endoplasmic reticulum in relation to mitochondria?
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Which statement correctly describes the staining characteristic of the cytoplasm in cells with abundant RER?
Which statement correctly describes the staining characteristic of the cytoplasm in cells with abundant RER?
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What is the primary function of phagocytosis?
What is the primary function of phagocytosis?
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Which process involves the invagination of the cell membrane to form a vesicle containing extracellular fluid?
Which process involves the invagination of the cell membrane to form a vesicle containing extracellular fluid?
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What activates the formation of coated vesicles during receptor-mediated endocytosis?
What activates the formation of coated vesicles during receptor-mediated endocytosis?
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During which process does the cell membrane fuse with a vesicle to release its contents outside the cell?
During which process does the cell membrane fuse with a vesicle to release its contents outside the cell?
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Which type of vesicle is typically involved in the signaling process inside a cell after endocytosis?
Which type of vesicle is typically involved in the signaling process inside a cell after endocytosis?
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How do receptor-mediated endocytosis and regular endocytosis differ?
How do receptor-mediated endocytosis and regular endocytosis differ?
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Which protein is commonly associated with coated vesicles involved in the endocytic process?
Which protein is commonly associated with coated vesicles involved in the endocytic process?
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What occurs to the ligands after they are internalized in receptor-mediated endocytosis?
What occurs to the ligands after they are internalized in receptor-mediated endocytosis?
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What distinguishes the rough endoplasmic reticulum (RER) from the smooth endoplasmic reticulum (SER)?
What distinguishes the rough endoplasmic reticulum (RER) from the smooth endoplasmic reticulum (SER)?
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Which subunit of the ribosome is responsible for the exit of the polypeptide chain during translation?
Which subunit of the ribosome is responsible for the exit of the polypeptide chain during translation?
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What role does the smooth endoplasmic reticulum (SER) play in muscle cells?
What role does the smooth endoplasmic reticulum (SER) play in muscle cells?
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How does the rough endoplasmic reticulum (RER) differ in cells that primarily produce hemoglobin?
How does the rough endoplasmic reticulum (RER) differ in cells that primarily produce hemoglobin?
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What is the primary function of the endoplasmic reticulum lumen?
What is the primary function of the endoplasmic reticulum lumen?
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Which enzyme is involved in the detoxification of harmful exogenous molecules in the smooth endoplasmic reticulum?
Which enzyme is involved in the detoxification of harmful exogenous molecules in the smooth endoplasmic reticulum?
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What characterizes the appearance of the cytoplasm in cells abundant with rough endoplasmic reticulum when stained with H&E?
What characterizes the appearance of the cytoplasm in cells abundant with rough endoplasmic reticulum when stained with H&E?
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In which type of cell is the smooth endoplasmic reticulum (SER) especially well developed for its role in contraction?
In which type of cell is the smooth endoplasmic reticulum (SER) especially well developed for its role in contraction?
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What is the primary function of lipid droplets in cells?
What is the primary function of lipid droplets in cells?
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What is true about glycogen granules?
What is true about glycogen granules?
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What is the condition called when a cell has one set of chromosomes?
What is the condition called when a cell has one set of chromosomes?
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Which statement accurately describes necrosis?
Which statement accurately describes necrosis?
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Which function best describes the role of exportins?
Which function best describes the role of exportins?
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What aspect distinguishes sex chromosomes from autosomes?
What aspect distinguishes sex chromosomes from autosomes?
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Which of the following accurately describes a Barr body?
Which of the following accurately describes a Barr body?
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What is one of the controlling mechanisms of apoptosis?
What is one of the controlling mechanisms of apoptosis?
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Which type of epithelial tissue is responsible for forming glands that secrete substances?
Which type of epithelial tissue is responsible for forming glands that secrete substances?
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What is the significance of pigment deposits like melanin granules in cells?
What is the significance of pigment deposits like melanin granules in cells?
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What is the main function of the nucleolus within the cell's nucleus?
What is the main function of the nucleolus within the cell's nucleus?
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What is a primary characteristic of epithelial tissue compared to connective tissue?
What is a primary characteristic of epithelial tissue compared to connective tissue?
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How many pairs of autosomes does a normal human karyotype have?
How many pairs of autosomes does a normal human karyotype have?
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What is the primary structure surrounding the nucleus?
What is the primary structure surrounding the nucleus?
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What is the role of the basement membrane for epithelial cells?
What is the role of the basement membrane for epithelial cells?
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Which component facilitates the bidirectional traffic between the nucleus and the cytoplasm?
Which component facilitates the bidirectional traffic between the nucleus and the cytoplasm?
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Which component of the basement membrane allows epithelial cells to connect to their environment?
Which component of the basement membrane allows epithelial cells to connect to their environment?
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What happens to telomeres with each cell division?
What happens to telomeres with each cell division?
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What is the primary function of the apical domain of epithelial cells?
What is the primary function of the apical domain of epithelial cells?
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What term describes the display of an organism's complete set of chromosomes?
What term describes the display of an organism's complete set of chromosomes?
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Which component of the nucleolus is primarily involved in the assembly of ribosomal subunits?
Which component of the nucleolus is primarily involved in the assembly of ribosomal subunits?
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In contrast to epithelial tissue, which type of tissue does not have polarized cells?
In contrast to epithelial tissue, which type of tissue does not have polarized cells?
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Which connective tissue component is essential for the mechanical stability of epithelial tissues?
Which connective tissue component is essential for the mechanical stability of epithelial tissues?
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What is the primary function of connective tissue in relation to other tissues?
What is the primary function of connective tissue in relation to other tissues?
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During which phase do sister chromatids separate and move towards opposite spindle poles?
During which phase do sister chromatids separate and move towards opposite spindle poles?
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What primarily causes the asymmetrical division of stem cells?
What primarily causes the asymmetrical division of stem cells?
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What structural component forms a cleavage furrow during cytokinesis?
What structural component forms a cleavage furrow during cytokinesis?
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What do kinetochores attach to during metaphase?
What do kinetochores attach to during metaphase?
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What is the primary role of stem cells in tissues?
What is the primary role of stem cells in tissues?
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What occurs to the nuclear envelope during telophase?
What occurs to the nuclear envelope during telophase?
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What describes the primary difference between stem cells and progenitor cells?
What describes the primary difference between stem cells and progenitor cells?
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Which phase of mitosis is characterized by the further condensation of chromosomes?
Which phase of mitosis is characterized by the further condensation of chromosomes?
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Flashcards
Endocytosis
Endocytosis
The process of bringing substances from outside the cell into the cell's interior.
Phagocytosis
Phagocytosis
A type of endocytosis where the cell engulfs large particles, like bacteria, into a phagosome.
Pinocytosis
Pinocytosis
A type of endocytosis where the cell takes in small droplets of extracellular fluid into a vesicle.
Receptor-mediated endocytosis
Receptor-mediated endocytosis
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Exocytosis
Exocytosis
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Coated Vesicles
Coated Vesicles
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Clathrin
Clathrin
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Cell Signaling Through Vesicles
Cell Signaling Through Vesicles
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Motor proteins
Motor proteins
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Integrins
Integrins
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Intermediate filaments
Intermediate filaments
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Mechanotransduction
Mechanotransduction
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Tissue engineering
Tissue engineering
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30S subunit
30S subunit
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60S subunit
60S subunit
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rRNA
rRNA
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Mitosis
Mitosis
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Interphase
Interphase
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G0 Phase
G0 Phase
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DNA Replication
DNA Replication
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Prophase
Prophase
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Mitochondrial Cristae
Mitochondrial Cristae
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Mitochondrial Matrix
Mitochondrial Matrix
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Endosymbiotic Theory
Endosymbiotic Theory
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Krebs Cycle in Mitochondria
Krebs Cycle in Mitochondria
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Electron Transport Chain (ETC)
Electron Transport Chain (ETC)
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ATP Synthase
ATP Synthase
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Peroxisomes
Peroxisomes
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Beta Oxidation
Beta Oxidation
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Secretory Epithelial Cells
Secretory Epithelial Cells
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Golgi Apparatus
Golgi Apparatus
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Cis-Face of Golgi
Cis-Face of Golgi
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Trans-Face of Golgi
Trans-Face of Golgi
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Lysosomes
Lysosomes
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Mitochondria
Mitochondria
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Outer Membrane of Mitochondria
Outer Membrane of Mitochondria
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Apoptosis
Apoptosis
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What are ribosomes and what is their function?
What are ribosomes and what is their function?
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Describe the three sites of a ribosome.
Describe the three sites of a ribosome.
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What is the endoplasmic reticulum and what are its main types?
What is the endoplasmic reticulum and what are its main types?
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What is the rough endoplasmic reticulum (RER) and what are its functions?
What is the rough endoplasmic reticulum (RER) and what are its functions?
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What is the smooth endoplasmic reticulum (SER) and what are its main roles?
What is the smooth endoplasmic reticulum (SER) and what are its main roles?
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What is the ER lumen?
What is the ER lumen?
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Explain the relationship between RER and secretory activities of cells.
Explain the relationship between RER and secretory activities of cells.
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Explain the role of SER in calcium regulation.
Explain the role of SER in calcium regulation.
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What are lipid droplets?
What are lipid droplets?
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What are glycogen granules?
What are glycogen granules?
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What are pigment deposits?
What are pigment deposits?
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What is necrosis?
What is necrosis?
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What is apoptosis?
What is apoptosis?
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What is the nuclear envelope?
What is the nuclear envelope?
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What are importins and exportins?
What are importins and exportins?
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What is the perinuclear cisterna?
What is the perinuclear cisterna?
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Sex chromosomes vs. Autosomes
Sex chromosomes vs. Autosomes
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Haploid vs. Diploid
Haploid vs. Diploid
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What is a karyotype?
What is a karyotype?
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What are FISH probes?
What are FISH probes?
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What is a Barr body?
What is a Barr body?
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What are telomeres?
What are telomeres?
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What is the nucleolus?
What is the nucleolus?
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What are the components of the nucleolus?
What are the components of the nucleolus?
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Describe the three sites of a ribosome: A, P, and E.
Describe the three sites of a ribosome: A, P, and E.
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What is the endoplasmic reticulum and its main types?
What is the endoplasmic reticulum and its main types?
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What is the rough endoplasmic reticulum (RER) and its functions?
What is the rough endoplasmic reticulum (RER) and its functions?
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What is the smooth endoplasmic reticulum (SER) and its main functions?
What is the smooth endoplasmic reticulum (SER) and its main functions?
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Explain the relationship between RER and the secretory activities of cells.
Explain the relationship between RER and the secretory activities of cells.
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What happens during metaphase?
What happens during metaphase?
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What happens during anaphase?
What happens during anaphase?
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What happens during telophase?
What happens during telophase?
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What is cytokinesis?
What is cytokinesis?
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What is asymmetrical cell division?
What is asymmetrical cell division?
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What are stem cells?
What are stem cells?
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What are tissues?
What are tissues?
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How does the extracellular environment affect stem cells?
How does the extracellular environment affect stem cells?
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Epithelial Tissue
Epithelial Tissue
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Secretory/Glandular Epithelia
Secretory/Glandular Epithelia
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Apical Domain
Apical Domain
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Lateral Domain
Lateral Domain
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Basal Domain
Basal Domain
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Basement Membrane
Basement Membrane
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Basal Lamina
Basal Lamina
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Reticular Lamina
Reticular Lamina
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Study Notes
Histology
- Histology is the study of the microscopic structure of normal tissues, enabling understanding of tissue function.
- Tissues are organized collections of cells with similar morphology (epithelial, muscular, nervous, connective).
- Cell size ranges from 10-30 micrometers.
- Microscopes are used to observe cells.
Light Transmission Microscopy
- Uses visible light passing through a sample.
- Objective lenses magnify and project the image to the eyepiece.
- Magnifications range from X40 to X400.
- Samples must be transparent.
- Tissue sections are prepared for viewing.
Tissue Preparation
- Fixation: Preserves tissue structure by cross-linking proteins and inactivating enzymes.
- Dehydration: Removing water using increasingly concentrated alcohol solutions (65% to 100%).
- Clearing: Replacing alcohol with an organic solvent (e.g., paraffin).
- Infiltration: Embedding tissue in melted paraffin wax.
- Embedding: Hardening the paraffin-infiltrated tissue in a mold.
- Trimming: Preparing the paraffin block for sectioning on a microtome.
Staining
- Sections are stained to visualize structures and substances.
- Hematoxylin and eosin (H&E) stain is commonly used.
- Cell nuclei stain blue, while most cytoplasm stains pink/red.
- PAS (periodic acid-Schiff) stains carbohydrates, either alone or with other molecules.
Immunochemistry
- Uses antibodies to target specific molecules (e.g., proteins) of interest.
- Antibodies bind to target antigens, allowing identification & visualization.
Microscopy Techniques
- Phase-contrast microscopy: Useful for observing unstained specimens.
- Confocal microscopy: Images one precise slice of the sample.
- Stereomicroscopy: Used to observe 3D, non-transparent objects.
- Super-resolution microscopy: STORM, STED, high-resolution images.
Electron Microscopy
- Transmission electron microscopy (TEM): 2D images of thin sections.
- Scanning electron microscopy (SEM): 3D images of surfaces.
The Cell
- The plasma membrane is a double layer of phospholipids with cholesterol and proteins.
- Cholesterol affects the fluidity and flexibility.
- Proteins facilitate recognition, signaling, and transport across the membrane.
- Glycosidic chains are present on phospholipids and proteins (glycolipids and glycoproteins).
- Hyaluronic acid creates a jelly-like layer outside the cell, providing cushioning and lubrication.
Membrane Transport
- Simple diffusion: Lipophilic and small uncharged molecules cross membranes passively.
- Channel/facilitated diffusion: Membrane proteins allow passage of specific chemicals.
- Active transport (pumps): Moves molecules against concentration gradients.
Cytoskeleton
- Microtubules: Hollow tubes formed by tubulin; crucial for cell division, intracellular transport, and structure.
- Microfilaments: Two strands of actin; important in cell movements, structure, and cell contraction.
- Intermediate filaments: Structural support for cells.
Organelles
- Ribosomes: Sites of protein synthesis.
- Endoplasmic reticulum (ER): Synthesis and modification of proteins; lipid metabolism.
- Golgi apparatus: Processing, sorting, and modifying proteins and lipids.
- Lysosomes: Contain enzymes for digestion/waste removal.
- Mitochondria: Energy production (ATP) through cellular respiration.
- Peroxisomes: Involved in oxidation reactions.
Cellular Inclusion
- Lipid droplets: Store lipids in cells.
- Glycogen granules: Store glycogen (energy).
- Pigment deposits: Protect cell nuclei (e.g., melanin).
- Hemosiderin granules: Store iron.
Cell Death
- Necrosis: Cell swelling and rupture; causes inflammation.
- Apoptosis: Programmed cell death, not inflammatory.
The Nucleus
- The nucleus is a double membrane-bound structure containing the cell's DNA.
- Pores regulate traffic between the nucleus and the cytoplasm.
- Chromatin (DNA complexed with histones) is organized into nucleosomes.
- Chromosomes condense during cell division.
- The nucleolus is the site of ribosome assembly.
Cell Cycle
- The cell cycle consists of interphase (G1, S, G2) and the mitotic phase (mitosis and cytokinesis).
- Cell cycle checkpoints are critical for regulated cell growth and division.
Tissues
- Tissues are organized groups of similar cells working together.
- Epithelial tissues: Cover body surfaces, line cavities, and form glands.
- Connective tissues: Provide support, structure, and connect tissues.
- Muscle tissues: Responsible for movement.
- Nervous tissues: Transmit electrical signals.
Types of Epithelia
- Covering/lining epithelia: Classified by cell shape (squamous, cuboidal, columnar) and layering (simple, stratified).
- Glandular epithelia: Specialized for secretion; unicellular (e.g., goblet cells) or multicellular (e.g., salivary glands, pancreas).
Specializations of Epithelial Cells
- Microvilli: Increase surface area for absorption.
- Stereocilia: Increase surface area (e.g., in the inner ear).
- Cilia: Movement of substances (e.g., in the respiratory tract).
- Cell-to-cell junctions (tight junctions, adherens junctions, desmosomes, gap junctions): attach, communicate, or block substances from passing between cells.
Connective Tissues
- Connective tissues have a matrix that contains ground substance (GAGs, proteoglycans, proteins) and fibers (collagen, elastic).
- Loose connective tissues: Support and connect tissues (e.g., lamina propria).
- Dense connective tissues: Support and provide strength and structure (e.g., tendons, ligaments).
- Cartilage: Tough, flexible support (hyaline, elastic, fibrocartilage).
- Bone: Hard, mineralized support (spongy, compact).
Specialised Cells
- Fibroblast: Maintain and synthesize collagen fibers.
- Macrophages: Phagocytic cells.
- Mast cells: Release histamine/heparin in inflammation.
- Lymphocytes(B-lymphocytes and T-lymphocytes): part of the immune system.
- Plasma cells: Antibody production.
- Osteoblasts: Produce bone matrix.
- Osteocytes: Maintain bone matrix.
- Osteoclasts: Resorb bone matrix.
Bone Remodeling
- A cyclical process of bone resorption and formation, important for maintaining bone calcium levels and adapting to stress.
- Involved in many physiological processes including fracture healing.
Types of Gland
- Exocrine Glands: Secrete products into ducts that empty onto epithelial surfaces (e.g., Sweat glands, salivary glands, goblet cells).
- Endocrine Glands: Secrete hormones directly into blood (e.g., Thyroid gland, adrenal glands, pancreas).
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Description
Test your knowledge on mitochondrial functions, the phases of the cell cycle, and cellular components. This quiz covers essential concepts related to interphase, somatic cells, and the endosymbiotic theory, among others. Perfect for students studying cell biology and biochemistry.