Mitochondria and Cellular Metabolism Quiz
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Questions and Answers

What happens to pyruvate when molecular oxygen is limiting?

  • It is stored as glycogen.
  • It undergoes complete oxidation in the Krebs’ cycle.
  • It is fermented into lactate to generate NADH. (correct)
  • It is converted into acetyl-CoA.
  • Where does non-shivering thermogenesis mainly occur in the body?

  • In the liver.
  • In skeletal muscle tissue.
  • In white adipose tissue.
  • In brown adipose tissue. (correct)
  • What role do mitochondria play in calcium homeostasis?

  • They degrade excess calcium into inactive forms.
  • They actively transport calcium ions out of the cell.
  • They convert calcium ions into ATP.
  • They store and release Ca2+ ions for signal transduction. (correct)
  • How do mitochondria regulate cell division?

    <p>By producing ATP and NADPH needed for new biomass generation. (A)</p> Signup and view all the answers

    What is the structural feature of the inner mitochondrial membrane?

    <p>It is thrown into cristae, increasing surface area. (D)</p> Signup and view all the answers

    What function do mitochondria serve in hormonal signaling?

    <p>They contribute to signaling involving estrogens. (C)</p> Signup and view all the answers

    In what manner do mitochondria reproduce?

    <p>Through binary fission. (C)</p> Signup and view all the answers

    What is the significance of mitochondrial genomic DNA (mtDNA)?

    <p>It can carry mutations that affect all descendant cells. (A)</p> Signup and view all the answers

    What is the primary function of mitochondria in cells?

    <p>ATP biosynthesis (D)</p> Signup and view all the answers

    Which of the following statements about mitochondria is true?

    <p>Mitochondria can constitute up to 25% of the cell volume in non-phototrophic organisms. (D)</p> Signup and view all the answers

    In which cellular process is pyruvate converted to acetyl-CoA?

    <p>Link reaction (C)</p> Signup and view all the answers

    Which organism is described as having no true mitochondria?

    <p>Henneguya zschokkei (C)</p> Signup and view all the answers

    What is the main site of the respiratory chain in mitochondria?

    <p>Inner membrane (D)</p> Signup and view all the answers

    What is the significance of the proton gradient in mitochondria?

    <p>It drives ATP biosynthesis. (A)</p> Signup and view all the answers

    Which metabolic pathway occurs in the mitochondrial matrix?

    <p>Krebs cycle (B)</p> Signup and view all the answers

    What is the primary type of ATP-generating enzyme found in mitochondria?

    <p>ATP phosphohydrolase (A)</p> Signup and view all the answers

    What is the primary role of chloroplasts in plant cells?

    <p>Site of photosynthesis and ATP synthesis (A)</p> Signup and view all the answers

    Which type of plastid is responsible for synthesizing and storing pigments?

    <p>Chromoplasts (B)</p> Signup and view all the answers

    What do gerontoplasts primarily signify in chloroplast development?

    <p>Aging and senescence of chloroplasts (D)</p> Signup and view all the answers

    Which plastids are known to store starch?

    <p>Amyloplasts (B)</p> Signup and view all the answers

    What is the primary function of the outer membrane of the mitochondrion?

    <p>Initiation of apoptosis upon breakdown (A)</p> Signup and view all the answers

    What triggers phototrophic electron transport in chloroplasts?

    <p>Chlorophyll pigments (D)</p> Signup and view all the answers

    Which feature is unique to the inner membrane of the mitochondrion?

    <p>Rich in cardiolipin (D)</p> Signup and view all the answers

    How do chloroplasts contribute to plant defense mechanisms?

    <p>By triggering apoptosis and releasing signaling compounds (B)</p> Signup and view all the answers

    Which of the following plastids can differentiate into other types?

    <p>Leucoplasts (B)</p> Signup and view all the answers

    What is the main role of the matrix within the mitochondrion?

    <p>Serving as the site of the Krebs cycle and pyruvate oxidation (A)</p> Signup and view all the answers

    In which organelle is the synthesis of fatty acids primarily conducted?

    <p>Chloroplasts (A)</p> Signup and view all the answers

    Which component is NOT found in human mitochondrial DNA?

    <p>23S rRNA (A)</p> Signup and view all the answers

    Which statement about mitochondrial ribosomes is accurate?

    <p>They consist of 70S and can synthesize mitochondrial proteins (B)</p> Signup and view all the answers

    Which molecule is primarily responsible for establishing the proton gradient in the intermembrane space of mitochondria?

    <p>Cardiolipin (C)</p> Signup and view all the answers

    What happens to a cell when the outer membrane of the mitochondrion is breached?

    <p>Initiation of apoptosis is triggered (D)</p> Signup and view all the answers

    What is the size of the human mitochondrial genome approximately?

    <p>16.5 kbp (A)</p> Signup and view all the answers

    What are the main pigments found in chloroplasts of the Streptophyta?

    <p>Chl a, Chl b, xanthophylls and carotenes (A)</p> Signup and view all the answers

    What is the correct structure found in chloroplasts that is comprised of stacks of thylakoids?

    <p>Granum (C)</p> Signup and view all the answers

    Which of the following statements is true about thylakoids?

    <p>Each thylakoid has a space inside it referred to as the lumen. (D)</p> Signup and view all the answers

    What term is used to describe the stack of thylakoids in chloroplasts?

    <p>Granum (C)</p> Signup and view all the answers

    What element separates the inner and outer membranes of chloroplasts?

    <p>Intermembrane space (B)</p> Signup and view all the answers

    Which of the following colors is represented by the prefix 'chloro-' in scientific terminology?

    <p>Green (B)</p> Signup and view all the answers

    Which pigments are used by chloroplasts of the Chlorophyta?

    <p>Chl a, Chl b, Chl c, xanthophylls and carotenes (D)</p> Signup and view all the answers

    Which structure is described as containing plastoglobulus and starch granules?

    <p>Stroma (D)</p> Signup and view all the answers

    What is the primary function of the cristae in mitochondria?

    <p>To increase surface area for ATP production (B)</p> Signup and view all the answers

    Which type of plastid is formed from chloroplasts?

    <p>Chromoplasts (D)</p> Signup and view all the answers

    Which of the following pigments are utilized by cyanoplasts of the Glaucophyta?

    <p>Chl a, xanthophylls, carotenes, and phycobilins (B)</p> Signup and view all the answers

    What is a role of cardiolipin in mitochondrial membranes?

    <p>To bind protons and maintain Δp (B)</p> Signup and view all the answers

    Where are ribosomes located within mitochondria?

    <p>Matrix (D)</p> Signup and view all the answers

    Which carotenoid does not contain oxygen?

    <p>Lycopene (D)</p> Signup and view all the answers

    What are the key differences between mtDNA and cpDNA?

    <p>mtDNA is inherited maternally; cpDNA is maternally or paternally (B)</p> Signup and view all the answers

    What type of non-phototrophic plastid is involved in lipid storage?

    <p>Elaioplast (A)</p> Signup and view all the answers

    Flashcards

    Mitochondria: Primary Role

    The main function of mitochondria is to generate energy for the cell through cellular respiration.

    Mitochondrial Respiration Site

    The process where mitochondria generate energy occurs on the inner membrane of the mitochondria.

    Proton-Motive Force in Mitochondria

    Mitochondrial respiration involves a proton gradient across the inner membrane, driving ATP production by ATP synthase.

    Kreb's Cycle Location

    The Kreb's cycle occurs in the mitochondrial matrix, utilizing pyruvate from glycolysis to generate energy.

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    Mitochondrial Number Variability

    Mitochondria can vary in number per cell, ranging from zero in some cells like erythrocytes to thousands in others like hepatocytes.

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    Organisms Without Mitochondria

    Some organisms, like the flagellate Monocercomonoides exilis and the myxosporean Henneguya zschokkei, have completely lost or modified mitochondria, highlighting their evolutionary significance.

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    Mitochondria Prevalence in Eukarya

    Mitochondria are present in most Eukarya, contributing significantly to the cell's overall volume, particularly in non-photosynthetic organisms.

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    Mitochondrial Origin

    The evolutionary history of mitochondria suggests their origin from a symbiotic relationship with bacteria, providing insights into the evolution of eukaryotic cells.

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    Fermentation in Mitochondria

    When oxygen is limited, cells switch to fermentation, where pyruvate is converted to lactate, generating NADH for anabolic processes. The only ATP produced is from glycolysis.

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    Non-Shivering Thermogenesis

    A process where mitochondria use their proton gradient to generate heat instead of ATP. This is crucial during cold temperatures.

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    Calcium Homeostasis in Mitochondria

    Mitochondria are the primary storage and release points for calcium ions, which are essential for signaling in cells.

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    Mitochondria in Cell Division

    Mitochondria provide essential building blocks (ATP and NADPH) for cell division, regulating the rate at which cells reproduce.

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    Mitochondrial Role in Apoptosis

    Mitochondria play a crucial role in apoptosis, or programmed cell death, by releasing specific signaling molecules.

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    Mitochondrial Steroid Biosynthesis

    Mitochondria produce and store key molecules for the synthesis of steroid hormones, like estrogen.

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    Heme Production in Mitochondria

    Mitochondria play a role in the production of heme, a component of hemoglobin in red blood cells.

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    Mitochondrial DNA (mtDNA)

    These small, circular pieces of DNA are located within the mitochondrial matrix and contain genes essential for mitochondrial function.

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    Outer Mitochondrial Membrane: Composition

    The outer membrane of a mitochondrion is like the cell's own membrane, with similar composition and functions, but with additional enzymes and transport proteins.

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    Outer Membrane: Importance

    The outer membrane is crucial for mitochondrial function and cell survival. Its breakdown can trigger programmed cell death.

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    Inner Mitochondrial Membrane: Function

    The inner membrane is where the energy production machinery of the mitochondrion resides, housing electron transport and ATP synthesis.

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    Cardiolipin in Inner Membrane

    Cardiolipin, a unique lipid in the inner membrane, helps maintain the proton gradient crucial for energy production.

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    Intermembrane Space: Function

    The intermembrane space is a compartment between the inner and outer membranes, where a proton gradient is established for ATP synthesis.

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    Mitochondrial Matrix: Location and Function

    The mitochondrial matrix is the gel-like substance within the inner membrane, containing enzymes for vital metabolic processes, including the Kreb's cycle.

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    mtDNA: Disease Association

    Human mtDNA contains genes that can cause diseases when damaged, highlighting the importance of its integrity.

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    What are plastids?

    Plastids are membrane-bound organelles found in plant cells and some algae. They are responsible for photosynthesis, storing pigments, and producing various molecules.

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    What are chloroplasts?

    Chloroplasts are the type of plastid responsible for photosynthesis. They contain chlorophyll, the pigment that captures light energy.

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    What are rhodoplasts?

    Rhodoplasts are plastids found in red algae. They contain pigments like phycoerythrin, which absorbs blue light and allows red algae to live in deep water.

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    What are cyanoplasts?

    Cyanoplasts, also known as cyanelles or muroplasts, are plastids found in Glaucophyta algae. They are very similar to cyanobacteria, suggesting a close evolutionary relationship.

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    What are etioplasts?

    Etioplasts are immature chloroplasts that have not yet been exposed to light. They lack chlorophyll and are typically found in plant seedlings before they emerge from the soil.

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    What are gerontoplasts?

    Gerontoplasts are senescing chloroplasts that are breaking down. This occurs as plants age or during the fall season.

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    What are chromoplasts?

    Chromoplasts are plastids that synthesize and store pigments other than chlorophyll. They give fruits and flowers their vibrant colors.

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    What are leucoplasts?

    Leucoplasts are plastids that store various substances, like starch, fats, and proteins. There are different types of leucoplasts based on their storage function.

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    Stroma

    The internal fluid-filled space of a chloroplast, similar to the cytoplasm of a cell but containing special molecules for photosynthesis.

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    Granum (plural: grana)

    A stack of flattened, disc-like sacs called thylakoids found within chloroplasts, where light-dependent reactions of photosynthesis take place.

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    Thylakoid

    A single, flattened, disc-like sac within a chloroplast, where the chlorophyll resides and light-dependent reactions occur. Think of a small coin or disc shaped like a tiny bag.

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    Lumen

    The space enclosed within a thylakoid, where the protons accumulate during photosynthesis.

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    Chloroplasts

    Photosynthetic organelles found in plant cells and algae, responsible for capturing light energy to synthesize sugars.

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    Rhodoplasts

    Organelles in some algae resembling chloroplasts, but containing different photosynthetic pigments, giving them a reddish hue.

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    Lamella or fret

    A type of thylakoid that is located in the stroma of a chloroplast and connects grana to each other.

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    Chloroplast DNA (cpDNA)

    The circular DNA molecule found in chloroplasts, containing genes for chloroplast functions.

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    What are cristae in mitochondria?

    The inner membrane of the mitochondrion folds inward to create shelf-like structures called cristae. These cristae increase the surface area of the inner membrane, allowing for more space for the electron transport chains and ATP synthases involved in energy production.

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    What is the key role of mitochondria?

    Mitochondria are found in nearly all eukaryotic cells. They are the powerhouses of the cell, generating energy through cellular respiration. They produce ATP, the primary energy currency of the cell.

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    Describe the structure of the outer mitochondrial membrane.

    The outer membrane of the mitochondrion is a phospholipid bilayer similar to the cell membrane, but with additional proteins for transporting molecules in and out.

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    What is the function of the inner mitochondrial membrane?

    The inner membrane surrounds the mitochondrial matrix. It's highly folded and contains the electron transport chain, ATP synthase, carrier proteins, and other enzymes crucial for energy production.

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    What is mtDNA?

    mtDNA is a small, circular molecule of DNA found within the mitochondrial matrix. It encodes for some mitochondrial proteins, rRNA, and tRNA.

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    What is the main function of chloroplasts?

    Chloroplasts are organelles found in plant cells and some algae. They are responsible for photosynthesis, converting light energy into chemical energy in the form of glucose.

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    What is the structure of the inner membrane of chloroplasts?

    The inner membrane of the chloroplast is folded into flattened sacs called thylakoids, which are stacked into structures called grana. It's where the light-dependent reactions of photosynthesis occur.

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    Describe the stroma of a chloroplast.

    Chloroplasts contain a fluid-filled space called the stroma, where the Calvin cycle (light-independent reactions of photosynthesis) occurs. It also houses chloroplast DNA (cpDNA).

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    Study Notes

    Mitochondria and Plastids

    • Mitochondria and plastids are important cellular organelles with diverse roles
    • Popular science books discussing these organelles include "The Vital Question: Why Is Life the Way It Is?" by Lane (2015), "Power, Sex, Suicide: Mitochondria and the Meaning of Life" by Lane (2005), "Oxygen: The Molecule that Made the World" by Lane (2002), and "Eating the Sun: How Plants Power the Planet" by Morton (2009).

    Mitochondria

    • Most eukaryotic cells possess mitochondria, contributing significantly to cell volume (up to 25%).
    • The number of mitochondria per cell varies (e.g. >2,000 per hepatocyte in humans).
    • Some eukaryotic organisms lack mitochondria
    • Examples include: unicellular flagellate Monocercomonoides exilis and the multicellular Henneguya zschokkei
    • Mitochondria are the site of critical cellular processes.

    Mitochondria Roles

    • Respiratory chain: The inner mitochondrial membrane houses the electron transport chain, generating a proton-motive force crucial for ATP synthesis.
    • Krebs cycle: Pyruvate, from glycolysis, is converted to acetyl-CoA within the mitochondrial matrix, initiating the Krebs cycle.
    • Fermentation: Without sufficient oxygen, the Krebs cycle slows, and pyruvate is fermented into lactate, generating NADH.
    • Non-shivering thermogenesis: In brown adipose tissue, mitochondria can uncouple ATP synthesis from the proton gradient generating heat.
    • Calcium homeostasis: Mitochondria maintain calcium ion levels, crucial for cellular signaling.
    • Regulation of cell division: ATP and NADPH produced in mitochondria are necessary for cell biomass generation and division.
    • Other roles: Mitochondria also regulate apoptosis and participate in steroid, heme cofactor biosynthesis and hormonal signalling

    Mitochondrial Structures

    • Outer membrane: Contains porins, crucial for controlling passage of molecules.
    • Inner membrane: Highly folded to increase surface area and houses the respiratory chain proteins.
    • Intermembrane space: The region between the outer and inner membranes.
    • Matrix: The inner compartment of the mitochondrion containing ribosomes, mtDNA, and enzymes of the Krebs cycle.

    Mitochondrial Matrix

    • The matrix holds about 60% of the total soluble protein content, including pyruvate oxidation and Krebs cycle enzymes (excluding membrane-bound succinate dehydrogenase).
    • The matrix houses mitochondrial ribosomes(70s), comprising 50S and 30S subunits and contains mitochondrial DNA (mtDNA).
    • mtDNA contains genes for mitochondrial proteins and components of the oxidative phosphorylation system

    Plastids

    • Plastids are double-membrane-bound organelles found in plants and algae.
    • Plastids include chloroplasts, which enable photosynthesis, and other types of plastids like chromoplasts and leucoplasts.

    Chloroplasts

    • Site of phototrophic electron transport.
    • Chloroplasts are the site of photosynthesis, where light energy is converted into chemical energy (ATP and NADPH).
    • Plant Immunity: Chloroplasts produce chemicals that trigger apoptosis to defend against pathogens.
    • Fatty acid biosynthesis: Chloroplasts produce all plant-cell fatty acids.
    • Sugar and starch biosynthesis: Chloroplasts convert glucose into sucrose and starches.

    Chloroplast Membranes

    • Outer membrane: Similar composition to other membranes composed of phospholipids and galactolipids.
    • Inner membrane: Composed of galactolipids, phospholipids, and sulfolipids. Closely associated with light-harvesting systems.

    Chloroplast Stroma

    • The stroma is equivalent to the cytoplasm in bacterial cells
    • Contains plastoglobuli (lipid and protein structures) and enzymes involved in the Calvin-Benson-Bassham cycle (CO2 fixation).
    • Contains cpDNA (chloroplast DNA)

    Chromoplasts

    • Chromoplasts are plastids involved in fruit ripening and producing pigments in flowers
    • They originate from chloroplasts and produce carotenoids, anthocyanins, and other pigments.

    Chloroplasts vs. Rhodoplasts vs. Cyanoplasts

    • Different types of plastids have distinct pigment compositions that influence their functions.
    • Chloroplasts in different plant groups use different combinations of chlorophyll and carotenoid pigments to perform photosynthesis.

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    Test your knowledge on the fascinating roles of mitochondria in cellular processes, including respiration, calcium homeostasis, and thermogenesis. This quiz covers a variety of topics related to mitochondrial function and structure, ideal for biology students and enthusiasts.

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