18 Questions
What is the primary advantage of using incident illumination in fluorescence techniques?
It allows for the use of a wider range of objectives
What is the function of a dichroic mirror in incident illumination?
To reflect the desired wavelength of light to the object
What is the role of the objective in incident illumination?
It acts as a condenser and a numerical aperture
What is the primary function of an objective lens?
To collect maximum light from the object and form a high-quality real image
What is prevented from reaching the observer in incident illumination?
The excitation rays bouncing back from the slide and cover glass
What is the formula to calculate the numerical aperture (NA) of an objective lens?
NA = n x sin(u)
What is the term for the smallest distance between two dots or lines that can be seen as separate entities?
Resolution
What is the purpose of the selection filters in incident illumination?
To select the desired wavelength of light
Which type of objective lens is known for its simplicity and is most widely used?
Achromatic
What is the advantage of stimulating fluorescence on the observer's side of the preparation?
It makes the fluorescence more brilliant
What type of glass is often incorporated in apochromatic objective lenses?
Fluorite
What is the characteristic of planapochromat objective lenses?
Complex in structure with a total of 10 or more elements
What type of light source is commonly used in modern microscopes?
Incandescent tungsten-halogen bulb
What is the primary function of the condenser in a microscope?
To focus light on the object
What is the purpose of the aperture diaphragm in a condenser?
To control the diameter of light
What is the function of the object stage in a microscope?
To support the glass slides and allow light to pass through
What is the most important part of a microscope's equipment?
Objectives
How many lenses are typically found in an objective?
5-15
Learn about the numerical aperture of an objective lens, its impact on resolving details, and how it's calculated. Understand the role of refractive index and angle of the outermost ray in microscopy.
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