Microscopy: Numerical Aperture and Image Resolution
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Questions and Answers

What is the primary advantage of using incident illumination in fluorescence techniques?

  • It enables the use of transmitted light instead of reflected light
  • It reduces the amount of visible fluorescent light collected by the objective
  • It allows for the use of a wider range of objectives (correct)
  • It increases the intensity of the excitation beam

    • What is the function of a dichroic mirror in incident illumination?

  • To block the visible fluorescent light from reaching the observer
  • To split the excitation beam into two separate beams
  • To increase the numerical aperture of the objective
  • To reflect the desired wavelength of light to the object (correct)

    • What is the role of the objective in incident illumination?

  • It acts as a condenser but not as a numerical aperture
  • It acts only as a numerical aperture
  • It acts as a filter to select the desired wavelength
  • It acts as a condenser and a numerical aperture (correct)

    • What is the primary function of an objective lens?

    <p>To collect maximum light from the object and form a high-quality real image</p> Signup and view all the answers

    What is prevented from reaching the observer in incident illumination?

    <p>The excitation rays bouncing back from the slide and cover glass</p> Signup and view all the answers

    What is the formula to calculate the numerical aperture (NA) of an objective lens?

    <p>NA = n x sin(u)</p> Signup and view all the answers

    What is the term for the smallest distance between two dots or lines that can be seen as separate entities?

    <p>Resolution</p> Signup and view all the answers

    What is the purpose of the selection filters in incident illumination?

    <p>To select the desired wavelength of light</p> Signup and view all the answers

    Which type of objective lens is known for its simplicity and is most widely used?

    <p>Achromatic</p> Signup and view all the answers

    What is the advantage of stimulating fluorescence on the observer's side of the preparation?

    <p>It makes the fluorescence more brilliant</p> Signup and view all the answers

    What type of glass is often incorporated in apochromatic objective lenses?

    <p>Fluorite</p> Signup and view all the answers

    What is the characteristic of planapochromat objective lenses?

    <p>Complex in structure with a total of 10 or more elements</p> Signup and view all the answers

    What type of light source is commonly used in modern microscopes?

    <p>Incandescent tungsten-halogen bulb</p> Signup and view all the answers

    What is the primary function of the condenser in a microscope?

    <p>To focus light on the object</p> Signup and view all the answers

    What is the purpose of the aperture diaphragm in a condenser?

    <p>To control the diameter of light</p> Signup and view all the answers

    What is the function of the object stage in a microscope?

    <p>To support the glass slides and allow light to pass through</p> Signup and view all the answers

    What is the most important part of a microscope's equipment?

    <p>Objectives</p> Signup and view all the answers

    How many lenses are typically found in an objective?

    <p>5-15</p> Signup and view all the answers

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