Microscopy Basics: Understanding Microscopes

What is the main purpose of microscopy?

To get a magnified image of objects too small to be seen with the naked eye

What is the ratio of the size of an object seen under a microscope to the actual size observed with the unaided eye?

Magnification

What is the ability to differentiate two close points as separate?

Resolving power

Which type of microscopy uses electrons to form an image?

Transmission Electron Microscopy (TEM) Signup and view all the answers

Who constructed the first simple compound microscope in 1590?

F.H Janssen & Z.Janssen Signup and view all the answers

What is the minimum distance between two dots that can be separated by an electron microscope?

0.5 nm Signup and view all the answers

What is the range of magnification power of a light microscope?

10x to 80x Signup and view all the answers

What is the primary function of the ocular lens in a compound light microscope?

To magnify the image a second time Signup and view all the answers

Who built the first Electron Microscope in 1931?

Ernst Ruska Signup and view all the answers

What is the main source of illumination in an Electron Microscope?

A beam of accelerated electrons Signup and view all the answers

What is the resolution of images in an Electron Microscope?

Nanometers Signup and view all the answers

What is the main purpose of a Bright Field Light Microscope?

To inspect large specimens Signup and view all the answers

What is the purpose of the diopter adjustment in a microscope?

To correct for any difference in vision between your two eyes Signup and view all the answers

What is the function of the coarse adjustment in a microscope?

To bring the specimen into general focus Signup and view all the answers

What is the purpose of the stage clips in a microscope?

To hold the slide in place Signup and view all the answers

What is the function of the aperture in a microscope?

To allow light from the illuminator to reach the specimen Signup and view all the answers

What is the purpose of the nosepiece in a microscope?

To select different objective lenses Signup and view all the answers

What is the function of the fine adjustment in a microscope?

To fine tune the focus and increase the detail of the specimen Signup and view all the answers

Who is credited with the development of a simple microscope with a magnification of 200x – 300x?

Anton Von Leeuwenhoek Signup and view all the answers

What was the breakthrough in technology that increased the magnification of microscopes from about 1000x to 250,000x or more?

The introduction of electron beam microscopes Signup and view all the answers

What is the main difference between a simple optical microscope and a compound optical microscope?

The number of lenses used Signup and view all the answers

What is the purpose of a stereo microscope?

To view samples in 3D Signup and view all the answers

What is the term coined by Faber in 1623?

Microscope Signup and view all the answers

What does the eyepiece or ocular lens do in a compound optical microscope?

It combines with the objective lens to magnify the sample Signup and view all the answers