Microscopy Basics Quiz

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Questions and Answers

What is the total magnification when viewing a specimen with a 10x eyepiece and a 40x objective lens?

400x (correct)

50x

200x

100x

Which parts of a compound microscope are primarily responsible for magnification?

Eyepiece and Objective Lens (correct)

Stage and Stage Micrometer

Body Tube and Base

Base and Arm

What would be the formula to calculate the calibration factor using the ocular micrometer when only part of the micrometers are visible in the field of view?

CF = Total magnification/Field number

CF = Eyepiece magnification + Objective magnification

CF = Ocular micrometer/Stage micrometer unit (10um)

CF = Stage micrometer unit (10um)/Ocular micrometer unit (correct)

What does the term 'actual size' refer to in microscopy?

The size of a specimen measured under the microscope Signup and view all the answers

Which of the following is NOT a mechanical part of a microscope?

Eyepiece Signup and view all the answers

In the context of microscope usage, what does FOV stand for?

Field of View Signup and view all the answers

What is the function of a micrometer in microscopy?

To measure the size of an object viewed under the microscope Signup and view all the answers

How is the field of view affected by changing the objective lens on a microscope?

It decreases as higher magnification is used. Signup and view all the answers

What is the purpose of the coarse knob on a microscope?

To move the stage up and down quickly Signup and view all the answers

Which of the following types of microscopes can achieve the highest magnification?

Electron Microscope Signup and view all the answers

What is the maximum magnification power of a compound light microscope?

1000x Signup and view all the answers

In which type of microscope is the iris diaphragm found?

Compound Light Microscope Signup and view all the answers

Which part of the microscope serves to hold the slide in place?

Stage Signup and view all the answers

What distinguishes a binocular microscope from a monocular microscope?

The number of eyepieces Signup and view all the answers

What is the primary function of the eyepiece in a microscope?

To magnify the image captured by the objective lenses Signup and view all the answers

What happens to the image produced by a Transmission Electron Microscope?

It appears in 2D format Signup and view all the answers

Which microscope utilizes a beam of electrons to create magnified images?

Electron Microscope Signup and view all the answers

What is the main purpose of the light source in electric microscopes?

To provide illumination for viewing the specimen Signup and view all the answers

Study Notes

Microscopy Basics

Microscopes are essential tools for viewing microorganisms, which are invisible to the naked eye.
The H.M. Revolving Nosepiece holds objective lenses, allowing for quick changes in magnification.
The stage supports the specimen slide while the stage clip secures it in place.

Microscopic Components

Coarse Knob (larger) adjusts stage height for initial focusing, suitable for low power objectives (LPO).
Fine Adjustment Knob (smaller) provides precise focusing for high power objectives (HPO) and oil immersion objectives (OIO).

Magnifying Parts

Objective Lens magnifies the image with specific powers: Scanner (4x), LPO (10x), HPO (40x), OIO (100x).
Eyepiece/Ocular Lens typically has a magnification of 10x.

Types of Microscopes

Compound Light Microscope: Features both ocular and objective lenses, maximum magnification of 1000x.
Stereoscope (Dissecting Microscope): Provides lower magnification (up to 100x) for observing surface details.
Electron Microscope: Utilizes electrons for high-resolution images, with maximum magnification up to 50,000x (Transmission EM for 2D images; Scanning EM for 3D images).
Atomic Force Microscope: Detects surface topology with a maximum magnification of 1,000,000x.
Confocal Microscope: Produces images by illuminating thin planes, coupling laser and fluorescence techniques.

Mechanical Parts of Microscope

The base stabilizes the microscope; the body tube allows light travel from the objective to ocular lens; the arm serves as a handle for transport.

Total Magnification and Field of View

Total Magnification (TM) is calculated as TM = (eyepiece magnification) x (objective magnification).
Example calculation: (10x)(40x) results in a TM of 400x.
Field of View (FOV): the visible area through lenses, calculated as FOV = Field number / Total Magnification.

Micrometry and Measurement Techniques

Micrometry measures the size of microscopic objects; a micrometer is both an instrument and a unit (micrometers, µm).
Two techniques for measuring size:
- Estimated Size (ES): Calculated as ES = FOV / Number of specimens across.
- Actual Size (AS):
  - Step 1: Determine Calibration Factor (CF) using a stage micrometer and ocular micrometer.
    - CF is calculated differently depending on the visibility of micrometers in FOV.
  - Step 2: Calculate AS using the formula AS = (CF) x (Ocular units occupied).

Calibration Factor Formulas

Formula 1: CF = Stage micrometer unit (10µm) / Ocular micrometer unit (when micrometers are partially visible).
Formula 2: CF = Stage micrometer / Ocular micrometer (when micrometers are fully visible).

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Description

Test your knowledge on the fundamentals of microscopy. This quiz covers essential components of microscopes, magnification techniques, and different types of microscopes. Perfect for students seeking to understand how to utilize these important scientific tools.