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Questions and Answers
What scientific term refers to the ability to distinguish between objects that are close together when using a microscope?
What scientific term refers to the ability to distinguish between objects that are close together when using a microscope?
- Refraction
- Diffraction
- Resolution (correct)
- Magnification
Which type of microscope uses light to form an image, limiting its resolution?
Which type of microscope uses light to form an image, limiting its resolution?
- Scanning probe microscopes
- Laser scanning confocal microscopes
- Optical microscopes (correct)
- Electron microscopes
What is the maximum useful magnification of optical microscopes?
What is the maximum useful magnification of optical microscopes?
- 50x
- 500x
- 5000x
- 1500x (correct)
Which organelles can optical microscopes be used to observe?
Which organelles can optical microscopes be used to observe?
What type of microscope greatly increases the resolution compared to optical microscopes?
What type of microscope greatly increases the resolution compared to optical microscopes?
What is the maximum resolution of electron microscopes?
What is the maximum resolution of electron microscopes?
What is the maximum useful magnification of electron microscopes?
What is the maximum useful magnification of electron microscopes?
What type of electron microscope uses electromagnets to focus a beam of electrons?
What type of electron microscope uses electromagnets to focus a beam of electrons?
What makes denser parts of the specimen appear darker in the final image produced by transmission electron microscopes?
What makes denser parts of the specimen appear darker in the final image produced by transmission electron microscopes?
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Study Notes
Microscope Resolution and Types
- The scientific term that refers to the ability to distinguish between objects that are close together when using a microscope is "resolution".
- Optical microscopes use light to form an image, which limits their resolution.
- The maximum useful magnification of optical microscopes is around 1000x.
Observations with Optical Microscopes
- Optical microscopes can be used to observe organelles such as mitochondria and chloroplasts.
Electron Microscopes
- Electron microscopes greatly increase the resolution compared to optical microscopes.
- The maximum resolution of electron microscopes is around 0.1-1 nanometers.
- The maximum useful magnification of electron microscopes is around 100,000x.
- Transmission electron microscopes (TEM) use electromagnets to focus a beam of electrons.
- In TEM, denser parts of the specimen appear darker in the final image because they scatter more electrons, reducing the intensity of the transmitted beam.
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