9 Questions
What scientific term refers to the ability to distinguish between objects that are close together when using a microscope?
Resolution
Which type of microscope uses light to form an image, limiting its resolution?
Optical microscopes
What is the maximum useful magnification of optical microscopes?
1500x
Which organelles can optical microscopes be used to observe?
Mitochondria
What type of microscope greatly increases the resolution compared to optical microscopes?
Electron microscopes
What is the maximum resolution of electron microscopes?
0.0002 µm or 0.2 nm
What is the maximum useful magnification of electron microscopes?
×1,500,000
What type of electron microscope uses electromagnets to focus a beam of electrons?
Transmission electron microscopes (TEMs)
What makes denser parts of the specimen appear darker in the final image produced by transmission electron microscopes?
They absorb more electrons
Study Notes
Microscope Resolution and Types
- The scientific term that refers to the ability to distinguish between objects that are close together when using a microscope is "resolution".
- Optical microscopes use light to form an image, which limits their resolution.
- The maximum useful magnification of optical microscopes is around 1000x.
Observations with Optical Microscopes
- Optical microscopes can be used to observe organelles such as mitochondria and chloroplasts.
Electron Microscopes
- Electron microscopes greatly increase the resolution compared to optical microscopes.
- The maximum resolution of electron microscopes is around 0.1-1 nanometers.
- The maximum useful magnification of electron microscopes is around 100,000x.
- Transmission electron microscopes (TEM) use electromagnets to focus a beam of electrons.
- In TEM, denser parts of the specimen appear darker in the final image because they scatter more electrons, reducing the intensity of the transmitted beam.
Test your knowledge about the analysis of cell components and observation of organelles using microscopes. Understand the scientific terms of magnification and resolution and their importance in microscopy.
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