Microscopy and Microscopes Quiz
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Questions and Answers

What scientific term refers to the ability to distinguish between objects that are close together when using a microscope?

  • Refraction
  • Diffraction
  • Resolution (correct)
  • Magnification
  • Which type of microscope uses light to form an image, limiting its resolution?

  • Scanning probe microscopes
  • Laser scanning confocal microscopes
  • Optical microscopes (correct)
  • Electron microscopes
  • What is the maximum useful magnification of optical microscopes?

  • 50x
  • 500x
  • 5000x
  • 1500x (correct)
  • Which organelles can optical microscopes be used to observe?

    <p>Mitochondria</p> Signup and view all the answers

    What type of microscope greatly increases the resolution compared to optical microscopes?

    <p>Electron microscopes</p> Signup and view all the answers

    What is the maximum resolution of electron microscopes?

    <p>0.0002 µm or 0.2 nm</p> Signup and view all the answers

    What is the maximum useful magnification of electron microscopes?

    <p>×1,500,000</p> Signup and view all the answers

    What type of electron microscope uses electromagnets to focus a beam of electrons?

    <p>Transmission electron microscopes (TEMs)</p> Signup and view all the answers

    What makes denser parts of the specimen appear darker in the final image produced by transmission electron microscopes?

    <p>They absorb more electrons</p> Signup and view all the answers

    Study Notes

    Microscope Resolution and Types

    • The scientific term that refers to the ability to distinguish between objects that are close together when using a microscope is "resolution".
    • Optical microscopes use light to form an image, which limits their resolution.
    • The maximum useful magnification of optical microscopes is around 1000x.

    Observations with Optical Microscopes

    • Optical microscopes can be used to observe organelles such as mitochondria and chloroplasts.

    Electron Microscopes

    • Electron microscopes greatly increase the resolution compared to optical microscopes.
    • The maximum resolution of electron microscopes is around 0.1-1 nanometers.
    • The maximum useful magnification of electron microscopes is around 100,000x.
    • Transmission electron microscopes (TEM) use electromagnets to focus a beam of electrons.
    • In TEM, denser parts of the specimen appear darker in the final image because they scatter more electrons, reducing the intensity of the transmitted beam.

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    Description

    Test your knowledge about the analysis of cell components and observation of organelles using microscopes. Understand the scientific terms of magnification and resolution and their importance in microscopy.

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