Microscopy and Digital Image Manipulation Quiz
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Questions and Answers

What is the main advantage of confocal laser-scanning microscopy (CLSM) compared to standard brightfield microscopy?

  • Limited spatial resolution
  • Ability to capture very dynamic cellular processes (correct)
  • Efficiency in capturing deep into thicker specimens
  • Higher magnification capabilities
  • How are images obtained with standard fluorescence microscopy different from those obtained with confocal laser-scanning microscopy (CLSM)?

  • They have higher spatial resolution in CLSM images.
  • CLSM captures images at a lower magnification than standard fluorescence microscopy.
  • CLSM excludes out-of-focus fluorescence from the specimen. (correct)
  • Standard fluorescence microscopy captures more dynamic processes than CLSM.
  • How does super-resolution CLSM differ from standard CLSM in terms of spatial resolution?

  • Both super-resolution and standard CLSM have similar spatial resolutions.
  • Super-resolution CLSM has spatial resolution about 10 times better than standard CLSM. (correct)
  • Standard CLSM has better spatial resolution than super-resolution CLSM.
  • Super-resolution CLSM has spatial resolution about 10 times worse than standard CLSM.
  • What is the primary limitation of super-resolution CLSM?

    <p>Longer specimen scanning time</p> Signup and view all the answers

    Why is point laser light potentially harmful in confocal laser-scanning microscopy (CLSM)?

    <p>It can photobleach fluorescent molecules and damage live cells due to phototoxicity.</p> Signup and view all the answers

    Which step involves the incorporation of vesicle (donor) membrane and lumenal cargo proteins into the acceptor compartment in the endomembrane system trafficking process?

    <p>Step 3</p> Signup and view all the answers

    Which transport pathway involves the transportation of materials from the endoplasmic reticulum to Golgi, and then to lysosomes (vacuoles in plants)?

    <p>Biosynthetic pathway</p> Signup and view all the answers

    What is the last step in the general process of trafficking through the endomembrane system via transport vesicles?

    <p>Step 4: Process of budding and fusion repeated</p> Signup and view all the answers

    In the exocytosis process, which type of secretion involves the release of materials in a regulated manner in response to specific signals?

    <p>Regulated secretion</p> Signup and view all the answers

    Which pathway involves the transport of materials from the Golgi or endosomes to the plasma membrane and extracellular space?

    <p>Exocytosis</p> Signup and view all the answers

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