Podcast
Questions and Answers
What is a primary limitation of light microscopes compared to electron microscopes?
What is a primary limitation of light microscopes compared to electron microscopes?
- Inability to study living cells
- Limited magnification and resolution (correct)
- Inability to view specimens at all
- Requires complex sample preparation
What can be observed clearly using electron microscopes that cannot be seen well with light microscopes?
What can be observed clearly using electron microscopes that cannot be seen well with light microscopes?
- Fine details within the nucleus (correct)
- Color variations in cytoplasm
- The outer cell membrane
- Large cellular structures
How do you calculate magnification according to the provided equation?
How do you calculate magnification according to the provided equation?
- Size of Image / Size of Real Object (correct)
- Size of Real Object / Size of Image
- Size of Image + Size of Real Object
- Size of Image - Size of Real Object
If the image size is 45 mm and the real object size is 0.01 mm, what is the magnification?
If the image size is 45 mm and the real object size is 0.01 mm, what is the magnification?
Which of the following statements is true regarding light microscopes?
Which of the following statements is true regarding light microscopes?
Flashcards
Light Microscope
Light Microscope
A microscope that uses light to view a specimen. It can magnify images, making them appear larger.
Electron Microscope
Electron Microscope
A microscope that uses electrons to view a specimen. It offers much higher magnification and resolution than a light microscope.
Magnification
Magnification
The ratio of the size of an image to the size of the real object. It tells you how much bigger the image is compared to the actual object.
Magnification Equation
Magnification Equation
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Resolution
Resolution
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Study Notes
Light Microscopes
- Light microscopes use light to view a specimen.
- Light microscopes have been used to study cells for hundreds of years, enabling important discoveries about cellular structures.
- Light microscopes magnify images, making them appear larger than the actual object.
- Limitations of light microscopes include: limited magnification, preventing easy viewing of structures inside the nucleus; and limited resolution, resulting in blurred images and inability to see fine detail.
Electron Microscopes
- Electron microscopes were developed to provide greater detail of cellular structures.
- Electron microscopes surpass light microscopes in magnification and resolution.
- This superior ability allows observation of details within structures such as the nucleus, invisible with light microscopes.
Magnification Calculation
- Equation: Magnification = Size of Image / Size of Real Object
- This equation calculates microscope magnification.
- The equation will be provided in exams, but the formula triangle will not be offered.
Examples of Calculations
- Example 1:
- Image size = 45 mm
- Real object size = 0.01 mm
- Magnification = 45 mm / 0.01 mm = 4,500 times
- Example 2:
- Image size = 30 mm
- Real object size = 0.03 mm
- Magnification = 30 mm / 0.03 mm = 1,000 times
- Example 3:
- Image size = 87 mm
- Magnification = 2,000 times
- Real object size = 87 mm / 2,000 = 0.0435 mm
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