Podcast Beta
Questions and Answers
What precautions should be taken when preparing a wet mount of a letter 'e' from a newspaper cut?
Avoid forming bubbles by lowering the cover slip starting from a 45o angle. Make sure to observe using both eyes and not lean on the oculars.
What should you do to care for the microscope?
Carry the microscope carefully to avoid misalignment, keep it dusted and clean, and inform an instructor if it's not working properly.
How can you determine the field of view of a microscope on the lowest power?
Place a clear plastic ruler with mm markings on the stage, focus under the lowest power objective, count ruler divisions across the diameter, and multiply by 1000 to get the field of view in micrometers.
What is the magnification when the lines of the ruler are clearly visible at 40X?
Signup and view all the answers
How can you accurately measure the field of view in a microscope?
Signup and view all the answers
What is the procedure for calibrating an ocular micrometer on a microscope?
Signup and view all the answers
Why is it important to calibrate an ocular micrometer for each different objective and microscope?
Signup and view all the answers
What is the purpose of using a stage micrometer in microscope calibration?
Signup and view all the answers
How is the field of view calculated on higher powers of a microscope?
Signup and view all the answers
What is the purpose of superimposing the zero lines of the ocular and stage micrometer scales during calibration?
Signup and view all the answers
Which of the following is the correct step to prevent bubbles from forming while preparing a wet mount on a microscope slide?
Signup and view all the answers
What care should be taken when handling a microscope to avoid misalignment?
Signup and view all the answers
What should you do if your microscope is not working properly according to the text?
Signup and view all the answers
How is the field of view in micrometers calculated using a ruler on a microscope?
Signup and view all the answers
What is the purpose of calibrating an ocular micrometer using a stage micrometer?
Signup and view all the answers
Why is calibrating an ocular micrometer essential before taking measurements?
Signup and view all the answers
What is the significance of multiplying the ocular units by the calibration factor during calibration?
Signup and view all the answers
When calibrating an ocular micrometer, what should be done after superimposing the lines of the stage and ocular micrometers?
Signup and view all the answers
What is the role of the stage micrometer in calibrating an ocular micrometer?
Signup and view all the answers
Why is it important to move one line to the edge of the field of view when measuring the field of view?
Signup and view all the answers