Podcast
Questions and Answers
What precautions should be taken when preparing a wet mount of a letter 'e' from a newspaper cut?
What precautions should be taken when preparing a wet mount of a letter 'e' from a newspaper cut?
Avoid forming bubbles by lowering the cover slip starting from a 45o angle. Make sure to observe using both eyes and not lean on the oculars.
What should you do to care for the microscope?
What should you do to care for the microscope?
Carry the microscope carefully to avoid misalignment, keep it dusted and clean, and inform an instructor if it's not working properly.
How can you determine the field of view of a microscope on the lowest power?
How can you determine the field of view of a microscope on the lowest power?
Place a clear plastic ruler with mm markings on the stage, focus under the lowest power objective, count ruler divisions across the diameter, and multiply by 1000 to get the field of view in micrometers.
What is the magnification when the lines of the ruler are clearly visible at 40X?
What is the magnification when the lines of the ruler are clearly visible at 40X?
How can you accurately measure the field of view in a microscope?
How can you accurately measure the field of view in a microscope?
What is the procedure for calibrating an ocular micrometer on a microscope?
What is the procedure for calibrating an ocular micrometer on a microscope?
Why is it important to calibrate an ocular micrometer for each different objective and microscope?
Why is it important to calibrate an ocular micrometer for each different objective and microscope?
What is the purpose of using a stage micrometer in microscope calibration?
What is the purpose of using a stage micrometer in microscope calibration?
How is the field of view calculated on higher powers of a microscope?
How is the field of view calculated on higher powers of a microscope?
What is the purpose of superimposing the zero lines of the ocular and stage micrometer scales during calibration?
What is the purpose of superimposing the zero lines of the ocular and stage micrometer scales during calibration?
Which of the following is the correct step to prevent bubbles from forming while preparing a wet mount on a microscope slide?
Which of the following is the correct step to prevent bubbles from forming while preparing a wet mount on a microscope slide?
What care should be taken when handling a microscope to avoid misalignment?
What care should be taken when handling a microscope to avoid misalignment?
What should you do if your microscope is not working properly according to the text?
What should you do if your microscope is not working properly according to the text?
How is the field of view in micrometers calculated using a ruler on a microscope?
How is the field of view in micrometers calculated using a ruler on a microscope?
What is the purpose of calibrating an ocular micrometer using a stage micrometer?
What is the purpose of calibrating an ocular micrometer using a stage micrometer?
Why is calibrating an ocular micrometer essential before taking measurements?
Why is calibrating an ocular micrometer essential before taking measurements?
What is the significance of multiplying the ocular units by the calibration factor during calibration?
What is the significance of multiplying the ocular units by the calibration factor during calibration?
When calibrating an ocular micrometer, what should be done after superimposing the lines of the stage and ocular micrometers?
When calibrating an ocular micrometer, what should be done after superimposing the lines of the stage and ocular micrometers?
What is the role of the stage micrometer in calibrating an ocular micrometer?
What is the role of the stage micrometer in calibrating an ocular micrometer?
Why is it important to move one line to the edge of the field of view when measuring the field of view?
Why is it important to move one line to the edge of the field of view when measuring the field of view?
