Microscope Wet Mount Preparation Instructions

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What precautions should be taken when preparing a wet mount of a letter 'e' from a newspaper cut?

Avoid forming bubbles by lowering the cover slip starting from a 45o angle. Make sure to observe using both eyes and not lean on the oculars.

What should you do to care for the microscope?

Carry the microscope carefully to avoid misalignment, keep it dusted and clean, and inform an instructor if it's not working properly.

How can you determine the field of view of a microscope on the lowest power?

Place a clear plastic ruler with mm markings on the stage, focus under the lowest power objective, count ruler divisions across the diameter, and multiply by 1000 to get the field of view in micrometers.

What is the magnification when the lines of the ruler are clearly visible at 40X?

40X magnification Signup and view all the answers

How can you accurately measure the field of view in a microscope?

Move one line to the edge of the field of view and use the given equation. Signup and view all the answers

What is the procedure for calibrating an ocular micrometer on a microscope?

Compare the ocular micrometer scale with a calibrated stage micrometer. Signup and view all the answers

Why is it important to calibrate an ocular micrometer for each different objective and microscope?

To determine the calibration factor specific to that setup. Signup and view all the answers

What is the purpose of using a stage micrometer in microscope calibration?

To provide a known scale for comparison. Signup and view all the answers

How is the field of view calculated on higher powers of a microscope?

Multiply the field of view at low power by the magnification of the higher power. Signup and view all the answers

What is the purpose of superimposing the zero lines of the ocular and stage micrometer scales during calibration?

To establish a reference point for accurate calibration. Signup and view all the answers

Which of the following is the correct step to prevent bubbles from forming while preparing a wet mount on a microscope slide?

Lower the cover slip slowly and start from a 45-degree angle. Signup and view all the answers

What care should be taken when handling a microscope to avoid misalignment?

Avoid bumping or jostling the microscope. Signup and view all the answers

What should you do if your microscope is not working properly according to the text?

Inform an instructor if the microscope is not working properly. Signup and view all the answers

How is the field of view in micrometers calculated using a ruler on a microscope?

Multiply the number of divisions by 1000. Signup and view all the answers

What is the purpose of calibrating an ocular micrometer using a stage micrometer?

To establish a calibration factor for different objectives and microscopes Signup and view all the answers

Why is calibrating an ocular micrometer essential before taking measurements?

To account for variations in microscope setups Signup and view all the answers

What is the significance of multiplying the ocular units by the calibration factor during calibration?

To calculate the size of objects accurately Signup and view all the answers

When calibrating an ocular micrometer, what should be done after superimposing the lines of the stage and ocular micrometers?

Identify where any two lines are exactly superimposed Signup and view all the answers

What is the role of the stage micrometer in calibrating an ocular micrometer?

To provide a calibrated scale for comparison Signup and view all the answers

Why is it important to move one line to the edge of the field of view when measuring the field of view?

To more accurately determine the field of view Signup and view all the answers