Questions and Answers
What are the functions of the proper use of the fine and coarse adjustment knobs of the microscope?
The coarse adjustment knob is used only in the scanning objective lens to move the stage up and down in bigger increments, bringing it closer to the lens faster. The fine adjustment knob is used on higher objectives to bring the image into sharper focus.
The final magnification of a specimen being viewed via a 10x ocular objective and a 40x objective lens is: _____
400x
Flat field objective lenses are also referred to as: _____
Planachromatic
The part of the microscope that functions to aim and focus the light through the specimen is the: _____
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The part of the microscope that functions to regulate the amount of light through the specimen is the: _____
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The final magnification of a specimen being viewed via a 10x ocular objective and a 50x objective lens is: _____
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What is the preferred cleaning solution for the routine cleaning of microscope lenses?
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What is the preferred cleaning solution for the removal of excess oil from microscope lenses?
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Mineral oil can be used in place of immersion oil with the oil-immersion lens.
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Discuss the key steps for the use, care, and maintenance of the microscope.
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Study Notes
Microscope Adjustment Knobs
- Coarse adjustment knob is primarily used with the scanning objective lens for quick focus adjustments.
- Fine adjustment knob is utilized with higher objective lenses, providing precise focus by moving the stage slightly.
Microscope Use and Maintenance
- Always lower the stage to its lowest point before starting.
- Inspect and clean eyepieces, objective lenses, and condenser lens as necessary, following the manufacturer's instructions.
- Properly position the slide on the stage with the correct side facing up.
- Adjust the distance between eyepieces for optimal viewing, aligning the two fields seamlessly.
- Use the coarse knob for lower objectives and the fine knob for higher objectives; avoid coarse adjustments with high-power or oil-immersion lenses.
- When using the x40 objective, locate a suitable area with the x10 lens, then switch and refine focus with the fine adjustment.
- Employ immersion oil only with the x100 lens, applying it while the lens is halfway between the high-power and oil-immersion positions.
Final Steps in Microscope Use
- After use, turn off the light, lower the stage completely, and return to the low-power objective.
- Carefully remove and clean the slide, and ensure the oil-immersion lens is cleaned if oil was used.
- Cover the microscope to protect it.
Magnification
- Final magnification with a 10x ocular and 40x objective lens is 400x.
- Final magnification with a 10x ocular and 50x objective lens is 500x.
Objective Lenses Terminology
- Flat field objective lenses are also known as planachromatic.
Key Microscopic Components
- The condenser directs and focuses light through the specimen.
- The iris diaphragm regulates the amount of light passing through the specimen.
Microscope Cleaning Solutions
- Methanol is the preferred solution for routine cleaning of microscope lenses.
- Xylene is effective for removing excess oil from microscope lenses.
Immersion Oil Misconceptions
- Mineral oil should not be used as a substitute for immersion oil with the oil-immersion lens; this statement is false.
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Description
Test your knowledge on the proper use and maintenance of microscope adjustment knobs. This quiz covers the functionality of coarse and fine adjustment knobs, as well as essential techniques for optimal microscope performance. Ensure you're familiar with best practices for cleaning and positioning slides for effective observation.
