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Questions and Answers
What is a simple microscope?
It's a single lens microscope that provides small magnifications.
Which of the following microscopes uses two lenses to magnify objects?
A fluorescence microscope generates an image using reflection of light.
False
What is the purpose of a phase-contrast microscope?
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Which microscope provides a three-dimensional view of a specimen?
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What type of radiation does an electron microscope use?
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The ______ lens is the primary magnifier of the microscope.
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What function does the diaphragm (iris) have in a microscope?
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Which part of the microscope helps to focus the image under high power?
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Match the following types of objective lenses with their magnification power:
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Study Notes
Microscope Types
- Simple Microscope: Single lens for low magnification, used to examine soil samples.
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Compound Microscope: Also known as an optical microscope, uses two lenses (objective and eyepiece) for higher magnification.
- Requires a partially translucent specimen for light to pass through.
- Light source can be either a mirror or an electric lamp.
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Types:
- Monocular: One eyepiece
- Binocular: Two eyepieces
- Trinocular: Three eyepieces
- Fluorescence Microscope: Uses fluorescence to generate an image.
- Phase-Contrast Microscope: Allows visualization of living, unstained cells and their structure.
- Stereo Microscope: Provides a 3D view of a specimen, also known as a dissecting microscope or stereo zoom microscope.
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Electron Microscope: Uses electrons instead of light to achieve higher magnification, up to 1000 times that of a compound microscope.
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Types:
- Scanning electron microscope (SEM): Examines the surface of cells and organisms.
- Transmission electron microscope (TEM): Provides detailed internal structure of cells.
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Types:
Parts of a Compound Microscope
- Arm: Connects the ocular tube and base, facilitates carrying.
- Base: Provides stability for the microscope.
- Coarse Adjustment Knobs: Move the stage up and down to focus under high power.
- Condenser: Concentrates light from the illuminator into a cone.
- Diaphragm (Iris): Regulates the intensity of light.
- Eyepiece (Ocular Lens): Magnifies the image produced by the objective lens.
- Fine Adjustment Knobs: Used for precise focusing under high power.
- Illuminator: Provides the light source.
- Mirror: Reflects light from an external source.
- Objective Lens: The primary lens that magnifies the specimen.
- Ocular Tube: Maintains the correct distance between the objective and eyepiece.
- Revolving Nose Piece: Holds the objective lenses and allows for changing magnification by rotating.
- Stage: Platform for holding the specimen slide.
- Stage Clips: Secure the slide onto the stage.
Objective Lenses Types
- Scanning Objective Lens (4x): Lowest magnification, provides a wide overview of the slide or "scan."
- Low Power Objective Lens (10x): Offers a more detailed view compared to the scanning lens.
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Description
Test your knowledge on the various types of microscopes and their functionalities. This quiz covers simple, compound, fluorescence, phase-contrast, stereo, and electron microscopes, among others. Determine your understanding of their uses and distinctions.
