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Questions and Answers
What is the primary use of a light microscope?
What is the primary use of a light microscope?
- Measuring the wavelength of light
- Magnifying larger objects such as insects
- Viewing very small objects at a molecular level
- Observing live samples with clarity (correct)
Which type of microscope uses electron beams to achieve higher magnification?
Which type of microscope uses electron beams to achieve higher magnification?
- Stereomicroscope
- Compound microscope
- Light microscope
- Electron microscope (correct)
What defines the magnification power of a microscope?
What defines the magnification power of a microscope?
- Ratio of the image size to the actual size of the object (correct)
- Length of the optical tube
- Number of ocular lenses
- The size of the stage
What is the function of the objective lens in a microscope?
What is the function of the objective lens in a microscope?
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Which microscope is typically not used for live specimen observation?
Which microscope is typically not used for live specimen observation?
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Study Notes
Light Microscope
- Primarily used for viewing live or preserved specimens, including biological samples like cells and tissues.
- Offers magnification ranging from 40x to 1000x, allowing details in specimens to be observed effectively.
Electron Microscope
- Utilizes electron beams instead of visible light to achieve much higher magnification, typically ranging from 1000x to 10,000,000x.
- Two main types: Transmission Electron Microscope (TEM) and Scanning Electron Microscope (SEM), each serving different imaging purposes.
Magnification Power
- Defined by the combination of ocular lens (eyepiece) and objective lens powers.
- Total magnification is calculated by multiplying the magnification of the ocular lens by that of the objective lens.
Objective Lens
- Critical component for focusing light on the specimen.
- Different objective lenses provide varying levels of magnification, allowing detailed examination of specimens at different resolutions.
Live Specimen Observation
- Electron microscopes are typically not used for observing live specimens due to the vacuum environment required for electron beam generation.
- This limitation precludes the use of electron microscopes for biological samples that require a living state.
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