18 Questions
The small divisions of the stage micrometer scale are 0.1 ____________
mm
A light microscope is used to observe two structures that are 200 ____________ apart.
nm
The limit of resolution is about half the wavelength of the radiation used to view the specimen. The shorter the ____________, the better the resolution.
wavelengths
An electron microscope exposes a specimen to ____________ instead of light.
electrons
As electrons are absorbed by molecules of air, a near-vacuum has to be created within the chamber of an electron microscope. Electron pass through and around the specimen, giving some ____________.
contrast
The image from an electron microscope is ____________ with a magnification of x500,000.
2D
The microscope in cell studies uses a beam of ______ to produce an image.
light
Most commonly used microscope for cell studies is the ______ microscope.
light
Iodine stained onion cells are used for studying ______ specimens.
thin
Methylene blue stained cheek cells are commonly used for cell ______.
study
The microscope passes light through the specimen and magnifies the image using glass ______.
lenses
In cell studies, it is important to draw smooth continuous lines with a sharp ______.
pencil
Electron microscopes are expensive and mainly used in __________ labs.
research
All electron microscopes cannot see in color because there is only one wavelength which is ________.
electrons
Ribosomes are not visible using a light microscope because of the difference in ________ used.
wavelength
Specimens in electron microscopes are coated in heavy ________ ions.
gold
The image produced by an electron microscope is 3D with a magnification of x100,000 because electrons do not pass through the sample but ________ off.
reflect
False-color images in electron microscopes can be created through computer coloring of the standard black and white image, providing a way to see the sample in ________.
color
Study Notes
- The stage micrometer scale is divided into small divisions of 0.1.
- The length of the nucleus in a plant cell can be determined by replacing the stage micrometer scale with a slide of a plant cell.
- When using a light microscope with a magnification of ×40 to ×400, two structures that are 200 nm apart will appear to be closer together.
- Electron microscopes use electrons instead of light to observe specimens, allowing for higher resolving power and the ability to distinguish objects as close as 0.5 nm apart.
- Electron microscopes require a near-vacuum environment for optimal imaging, and specimens are coated with heavy ions to enhance contrast.
Test your knowledge on microscope scale and magnification. Questions include determining the length of a nucleus in a plant cell and calculating the distance between two structures under different magnifications.
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