Microscope Scale and Magnification Quiz
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Questions and Answers

The small divisions of the stage micrometer scale are 0.1 ____________

mm

A light microscope is used to observe two structures that are 200 ____________ apart.

nm

The limit of resolution is about half the wavelength of the radiation used to view the specimen. The shorter the ____________, the better the resolution.

wavelengths

An electron microscope exposes a specimen to ____________ instead of light.

<p>electrons</p> Signup and view all the answers

As electrons are absorbed by molecules of air, a near-vacuum has to be created within the chamber of an electron microscope. Electron pass through and around the specimen, giving some ____________.

<p>contrast</p> Signup and view all the answers

The image from an electron microscope is ____________ with a magnification of x500,000.

<p>2D</p> Signup and view all the answers

The microscope in cell studies uses a beam of ______ to produce an image.

<p>light</p> Signup and view all the answers

Most commonly used microscope for cell studies is the ______ microscope.

<p>light</p> Signup and view all the answers

Iodine stained onion cells are used for studying ______ specimens.

<p>thin</p> Signup and view all the answers

Methylene blue stained cheek cells are commonly used for cell ______.

<p>study</p> Signup and view all the answers

The microscope passes light through the specimen and magnifies the image using glass ______.

<p>lenses</p> Signup and view all the answers

In cell studies, it is important to draw smooth continuous lines with a sharp ______.

<p>pencil</p> Signup and view all the answers

Electron microscopes are expensive and mainly used in __________ labs.

<p>research</p> Signup and view all the answers

All electron microscopes cannot see in color because there is only one wavelength which is ________.

<p>electrons</p> Signup and view all the answers

Ribosomes are not visible using a light microscope because of the difference in ________ used.

<p>wavelength</p> Signup and view all the answers

Specimens in electron microscopes are coated in heavy ________ ions.

<p>gold</p> Signup and view all the answers

The image produced by an electron microscope is 3D with a magnification of x100,000 because electrons do not pass through the sample but ________ off.

<p>reflect</p> Signup and view all the answers

False-color images in electron microscopes can be created through computer coloring of the standard black and white image, providing a way to see the sample in ________.

<p>color</p> Signup and view all the answers

Study Notes

  • The stage micrometer scale is divided into small divisions of 0.1.
  • The length of the nucleus in a plant cell can be determined by replacing the stage micrometer scale with a slide of a plant cell.
  • When using a light microscope with a magnification of ×40 to ×400, two structures that are 200 nm apart will appear to be closer together.
  • Electron microscopes use electrons instead of light to observe specimens, allowing for higher resolving power and the ability to distinguish objects as close as 0.5 nm apart.
  • Electron microscopes require a near-vacuum environment for optimal imaging, and specimens are coated with heavy ions to enhance contrast.

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Description

Test your knowledge on microscope scale and magnification. Questions include determining the length of a nucleus in a plant cell and calculating the distance between two structures under different magnifications.

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