Microscope Resolution

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Questions and Answers

What factor affects the resolution of an image by determining the ability to resolve smaller objects?

  • Air between the specimen and lens
  • Wavelength (correct)
  • Magnification
  • Numerical aperture

Why does an electron microscope have a much higher resolution than a light microscope?

  • Due to its higher numerical aperture
  • Due to its lower magnification capabilities
  • Because it uses shorter wavelengths (correct)
  • Because of the large refraction in air

What is the role of immersion oil in enhancing resolution at very high magnifications of a light microscope?

  • To enhance numerical aperture
  • To increase the refractive indices of light
  • To scatter light rays
  • To fill the space between the specimen and lens (correct)

Which of the following is NOT a factor that affects resolution according to the text?

<p>Magnification (C)</p> Signup and view all the answers

Why does resolution become compromised at very high magnifications in a light microscope?

<p>Because of the large refraction caused by air (C)</p> Signup and view all the answers

How does the numerical aperture affect image resolution?

<p>By gathering more light for better resolution (D)</p> Signup and view all the answers

In a light microscope, what is the function of an oil immersion lens?

<p>To fill the space between the specimen and the lens with oil (A)</p> Signup and view all the answers

What is the primary purpose of using immersion oil in microscopy?

<p>To improve the resolution and clarity of the image (C)</p> Signup and view all the answers

How does immersion oil affect the light path in a microscope?

<p>It increases the maximum angle at which light can strike the lens (D)</p> Signup and view all the answers

Which of the following statements about bacterial cell morphologies is true?

<p>Only a few distinct cell morphologies are commonly seen in bacteria (D)</p> Signup and view all the answers

What does the term 'cellular arrangement' refer to in the context of bacterial cells?

<p>The pattern formed by bacterial cells during cell division (C)</p> Signup and view all the answers

How many main types of cellular morphologies are described for bacteria in the text?

<p>6 (A)</p> Signup and view all the answers

Which of the following is NOT a characteristic used to describe bacterial cells under a microscope?

<p>Gram staining (D)</p> Signup and view all the answers

What is the difference between cellular morphology and cellular arrangement in bacteria?

<p>Cellular morphology refers to the shape of individual cells, while cellular arrangement refers to the pattern formed during cell division (A)</p> Signup and view all the answers

What is the disadvantage of not using immersion oil when observing bacteria at 100x magnification?

<p>The numerical aperture and resolution will be lower (B)</p> Signup and view all the answers

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