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15 Questions

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Microscope Resolution

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Questions and Answers

What factor affects the resolution of an image by determining the ability to resolve smaller objects?

Air between the specimen and lens

Wavelength (correct)

Magnification

Numerical aperture

Why does an electron microscope have a much higher resolution than a light microscope?

Due to its higher numerical aperture

Due to its lower magnification capabilities

Because it uses shorter wavelengths (correct)

Because of the large refraction in air

What is the role of immersion oil in enhancing resolution at very high magnifications of a light microscope?

To enhance numerical aperture

To increase the refractive indices of light

To scatter light rays

To fill the space between the specimen and lens (correct)

Which of the following is NOT a factor that affects resolution according to the text?

Magnification Signup and view all the answers

Why does resolution become compromised at very high magnifications in a light microscope?

Because of the large refraction caused by air Signup and view all the answers

How does the numerical aperture affect image resolution?

By gathering more light for better resolution Signup and view all the answers

In a light microscope, what is the function of an oil immersion lens?

To fill the space between the specimen and the lens with oil Signup and view all the answers

What is the primary purpose of using immersion oil in microscopy?

To improve the resolution and clarity of the image Signup and view all the answers

How does immersion oil affect the light path in a microscope?

It increases the maximum angle at which light can strike the lens Signup and view all the answers

Which of the following statements about bacterial cell morphologies is true?

Only a few distinct cell morphologies are commonly seen in bacteria Signup and view all the answers

What does the term 'cellular arrangement' refer to in the context of bacterial cells?

The pattern formed by bacterial cells during cell division Signup and view all the answers

How many main types of cellular morphologies are described for bacteria in the text?

6 Signup and view all the answers

Which of the following is NOT a characteristic used to describe bacterial cells under a microscope?

Gram staining Signup and view all the answers

What is the difference between cellular morphology and cellular arrangement in bacteria?

Cellular morphology refers to the shape of individual cells, while cellular arrangement refers to the pattern formed during cell division Signup and view all the answers

What is the disadvantage of not using immersion oil when observing bacteria at 100x magnification?

The numerical aperture and resolution will be lower Signup and view all the answers