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Questions and Answers
What is the primary function of the base of a microscope?
What is the primary function of the base of a microscope?
Which part of the microscope is used to tilt the instrument for better viewing angles?
Which part of the microscope is used to tilt the instrument for better viewing angles?
What is the purpose of the dust shield in a microscope?
What is the purpose of the dust shield in a microscope?
Which component of the microscope is primarily responsible for adjusting the focus?
Which component of the microscope is primarily responsible for adjusting the focus?
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What is the role of the revolving nosepiece in a microscope?
What is the role of the revolving nosepiece in a microscope?
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Which part of the microscope is located at the upper smaller end of the body tube?
Which part of the microscope is located at the upper smaller end of the body tube?
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Which component of the microscope is responsible for delicate focusing when using high power and oil immersion objectives?
Which component of the microscope is responsible for delicate focusing when using high power and oil immersion objectives?
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What should be routinely performed to maintain a microscope in good condition?
What should be routinely performed to maintain a microscope in good condition?
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What is the primary function of the stage in a microscope?
What is the primary function of the stage in a microscope?
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What is a common type of objective lens used in compound microscopes?
What is a common type of objective lens used in compound microscopes?
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Which objective provides the highest magnification for viewing microorganisms and requires immersion oil?
Which objective provides the highest magnification for viewing microorganisms and requires immersion oil?
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What is the role of the iris diaphragm in a microscope?
What is the role of the iris diaphragm in a microscope?
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Which component is located below the stage and is used to hold the mirror?
Which component is located below the stage and is used to hold the mirror?
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What type of objective is the shortest and offers a whole view of the specimen?
What type of objective is the shortest and offers a whole view of the specimen?
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Which part of the microscope is designed to focus and concentrate light rays onto the specimen?
Which part of the microscope is designed to focus and concentrate light rays onto the specimen?
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Which adjustment mechanism should be used first when focusing on a slide at low power?
Which adjustment mechanism should be used first when focusing on a slide at low power?
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What is the primary purpose of keeping the objective with the lowest linear magnification in place before returning the microscope?
What is the primary purpose of keeping the objective with the lowest linear magnification in place before returning the microscope?
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What should be used to clean the microscope's lenses to prevent smudging?
What should be used to clean the microscope's lenses to prevent smudging?
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Which of the following actions should be avoided when adjusting the microscope?
Which of the following actions should be avoided when adjusting the microscope?
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How can you achieve the best illumination in the field of vision when using the microscope?
How can you achieve the best illumination in the field of vision when using the microscope?
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What is one important precaution to take when handling the microscope?
What is one important precaution to take when handling the microscope?
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What does the field of vision refer to in microscope usage?
What does the field of vision refer to in microscope usage?
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When should the microscope be checked for defects or damage?
When should the microscope be checked for defects or damage?
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Which component of the microscope should be lowered fully before storage?
Which component of the microscope should be lowered fully before storage?
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Study Notes
Components of the Compound Microscope
- Coarse Adjustment Knob: Larger wheel for low power objective focusing.
- Fine Adjustment Knob: Smaller wheel for precision focusing with high power and oil immersion objectives.
- Stage: Square or circular platform with a center opening for slide placement during focusing.
- Clips: Paired structures on the stage to secure the slide in position.
- Mirror Rack: Located below the stage, attached to the pillar for mirror support.
- Ocular/Eyepiece: Found at the draw tube, used for viewing specimens.
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Objectives: Tube-like structures on the revolving nosepiece:
- Scanner: Shortest tube providing a whole view of the specimen.
- Low Power Objective (LPO): Longer tube magnifying the object.
- High Power Objective (HPO): Even longer tube with detailed specimen view.
- Oil Immersion Objective: Long tube with a high degree of magnification, used with immersion oil for microorganism study.
- Mirror: Positioned below the stage, directing light onto the specimen via concave and plane surfaces.
- Iris Diaphragm: Circular blades below the stage that adjust light intensity from the mirror on the specimen.
- Condenser: Lens beneath the stage opening, focuses light onto the specimen.
Structure of the Microscope
- Base: Y or U-shaped support for the microscope.
- Pillar: Short metal piece supporting the microscope structure.
- Handle/Arm: Curved metallic component for holding the microscope.
- Inclination Joint/Screw: Joint enabling tilting of the microscope.
- Body Tube: Cylindrical structure housing the dust shield and objectives.
- Draw Tube: Upper smaller end of the body tube that includes the eyepiece.
- Revolving Nosepiece: Circular structure attached to the body tube's lower end for objective attachment.
- Dust Shield: Circular structure above the nosepiece protecting the lenses.
- Adjustment Knob: Two pairs of wheels on the body tube for focusing adjustments.
Proper Care for the Microscope
- Ensure the microscope is clean, dry, and dust-free; cover when not in use.
- Carry the microscope by grasping the arm with one hand and the base with the other, avoiding sudden movements.
- Avoid touching lenses with fingers; clean with lens paper if dirty.
- Prevent harsh contact of the lens with slides or covers; do not force adjustments.
- Ensure all adjustments function smoothly; avoid excessive turning of knobs.
- Before storage, position the lowest magnification objective, lower the body tube, retract the mechanical stage, and set the mirror vertically.
- Inspect the microscope for damage before use, reporting any issues to the instructor.
Using the Microscope
- Position the microscope near the table edge and select an appropriate stool for comfortable viewing.
- Look through the eyepiece with both eyes open, using the 10x objective.
- Adjust the mirror angle to achieve optimal illumination in the field of vision, which is the circular area visible through the eyepiece.
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Description
This quiz covers the essential components of a microscope, focusing particularly on the coarse and fine adjustment knobs, as well as the stage. Test your knowledge on how these parts contribute to effective observation in scientific studies.
