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Questions and Answers
What is the formula to calculate the total magnification of an object under a microscope?
What is the formula to calculate the total magnification of an object under a microscope?
What happens to the field of view when switching from low power objective lens to a high power objective lens?
What happens to the field of view when switching from low power objective lens to a high power objective lens?
What is the purpose of using stains like Iodine, Crystal Violet, and Malachite Green in microscopy?
What is the purpose of using stains like Iodine, Crystal Violet, and Malachite Green in microscopy?
Which direction does an object appear to move under a microscope when it is moved to the right on a slide?
Which direction does an object appear to move under a microscope when it is moved to the right on a slide?
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What is the correct relationship between the coarse adjustment knob and fine adjustment knob in focusing an object under the microscope?
What is the correct relationship between the coarse adjustment knob and fine adjustment knob in focusing an object under the microscope?
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What happens to the image of a specimen under the microscope when viewed with a high power objective lens?
What happens to the image of a specimen under the microscope when viewed with a high power objective lens?
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How does the total magnification change when switching from a 10X objective lens to a 40X objective lens?
How does the total magnification change when switching from a 10X objective lens to a 40X objective lens?
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Which part of the microscope is responsible for controlling the amount of light passing through the specimen?
Which part of the microscope is responsible for controlling the amount of light passing through the specimen?
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Study Notes
Microscope Magnification and Mechanics
- Total magnification of an object is calculated by multiplying the eyepiece magnification by the objective lens magnification.
- When switching from low power to high power objective lens, the field of view decreases, showing a smaller area of the specimen in detail.
Staining Techniques in Microscopy
- Stains like Iodine, Crystal Violet, and Malachite Green enhance contrast in microscopy, helping to visualize structures within cells that would otherwise be difficult to see.
Object Movement and Focus
- An object viewed under a microscope appears to move in the opposite direction; if moved to the right on the slide, it will appear to move left in the microscope's field of view.
- The coarse adjustment knob is used for initial focusing, while the fine adjustment knob is used for precise focusing once the image is close to being clear.
Objective Lens Impact
- Viewing a specimen with a high power objective lens results in increased detail but a reduced field of view, making it easier to study specific features.
- Total magnification significantly increases when changing from a 10X objective lens (100X total) to a 40X objective lens (400X total).
Light Control in Microscopy
- The diaphragm is the part of the microscope responsible for controlling the amount of light that passes through the specimen, affecting visibility and contrast.
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Description
Test your knowledge on the parts of a microscope and their functions, including the eyepiece, objective lenses, arm, base, and more. Learn about total magnification, the differences between low power and high power lenses, and steps to focus an object under a microscope.
