Microorganisms in Food Science
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Questions and Answers

What is the primary reproduction mechanism for bacteria?

  • Budding
  • Replication Inside Host Cells
  • Produces Spores
  • Binary Fission (correct)
  • Viruses can grow in foods.

    False (B)

    Name one characteristic used to categorize bacteria.

    Cell type or growth capability.

    Bacteria can reproduce asexually through __________.

    <p>Binary Fission</p> Signup and view all the answers

    Match the following microorganisms with their growth ability in foods:

    <p>Bacteria = Yes Yeast = Yes Viruses = No Parasites = No</p> Signup and view all the answers

    Which group of microorganisms cannot grow in foods?

    <p>Viruses (D)</p> Signup and view all the answers

    Mould is categorized as a multicellular microorganism.

    <p>True (A)</p> Signup and view all the answers

    What are the growth phases of bacteria?

    <p>Lag Phase, Log Phase, Stationary Phase.</p> Signup and view all the answers

    In which condition do facultative anaerobes grow faster?

    <p>Aerobic conditions (B)</p> Signup and view all the answers

    Viruses contain traditional chromosomes.

    <p>False (B)</p> Signup and view all the answers

    What is binary fission?

    <p>Asexual reproduction in prokaryotes where the single circular chromosome replicates and the cell splits into two identical daughter cells.</p> Signup and view all the answers

    A __________ is the viral DNA that integrates into a bacterial genome during the lysogenic cycle.

    <p>prophage</p> Signup and view all the answers

    Match the following taxonomic levels with their order:

    <p>1 = Domain 2 = Kingdom 3 = Phylum 4 = Class 5 = Order 6 = Family 7 = Genus 8 = Species</p> Signup and view all the answers

    What is the maximum density of bacteria that can be achieved in foods?

    <p>9 log CFU/mL (B)</p> Signup and view all the answers

    Gram-negative bacteria appear purple after Gram staining.

    <p>False (B)</p> Signup and view all the answers

    What are the optimal growth temperatures for psychrotrophs?

    <p>20°C to 30°C</p> Signup and view all the answers

    Bacteria form endospores in response to ______ conditions.

    <p>harsh</p> Signup and view all the answers

    Match the following bacteria with their oxygen requirements:

    <p>Microaerophiles = Require low levels of oxygen Aerotolerant anaerobes = Do not use oxygen but can survive in its presence Facultative anaerobes = Can grow with or without oxygen Obligate aerobes = Require oxygen to grow</p> Signup and view all the answers

    Which category of bacteria thrive in extremely cold temperatures?

    <p>Psychrophiles (D)</p> Signup and view all the answers

    Bacterial spores can replicate while in their dormant state.

    <p>False (B)</p> Signup and view all the answers

    What is the optimal growth temperature for mesophiles?

    <p>37°C</p> Signup and view all the answers

    Flashcards

    Facultative Anaerobe Growth

    Facultative anaerobes grow faster in aerobic conditions because oxygen allows them to generate more ATP through aerobic respiration compared to fermentation or anaerobic respiration.

    Binary Fission vs. Mitosis

    Binary fission is asexual reproduction in prokaryotes with a single circular chromosome replicating. It splits into two identical daughter cells. Mitosis is cell division in eukaryotes with multiple chromosomes and distinct stages (prophase, metaphase, anaphase, telophase) for accurate chromosome segregation.

    Prokaryote vs. Eukaryote Chromosomes

    Prokaryotes typically have one single, circular chromosome. Eukaryotes have multiple, linear chromosomes located within a nucleus.

    Viral Genetic Material

    Viruses don't have chromosomes; they contain genetic material as DNA or RNA enclosed within a protein capsid.

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    Prophage & Food Safety

    A prophage is a viral DNA integrated into a bacterial genome during the lysogenic cycle of a bacteriophage. It can encode toxins, antimicrobial resistance, or other virulence factors, making the bacteria more pathogenic.

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    Prokaryote

    A single-celled organism without a nucleus or other membrane-bound organelles, such as bacteria.

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    Eukaryote

    A single-celled or multicellular organism with a nucleus and other membrane-bound organelles, such as yeast and mold.

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    Binary Fission

    Asexual reproduction in bacteria where a single cell splits into two identical daughter cells.

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    Budding

    Asexual reproduction in yeast where a new cell grows as an outgrowth from the parent cell.

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    Spore Formation

    Asexual reproduction in mold where specialized cells called spores are produced for dispersal and survival.

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    Lag Phase

    The initial phase of bacterial growth where cells adapt to their environment and prepare for division.

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    Log Phase

    The phase of bacterial growth where the population increases exponentially due to rapid cell division.

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    Stationary Phase

    The phase of bacterial growth where the rate of cell division equals the rate of cell death due to limited resources and waste accumulation.

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    Death Phase

    Stage of bacterial growth where the death rate exceeds the growth rate, caused by exhaustion of resources and toxic buildup.

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    Maximum Bacterial Density

    The highest concentration of bacteria achievable in a food environment, typically around 9 log CFU/mL (10^9 CFU/mL).

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    Typical Foodborne Bacteria

    Most foodborne pathogens are mesophiles (37°C optimal) and facultative anaerobes (can grow with or without oxygen).

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    Endospore Germination

    Endospores germinate into vegetative cells when favourable conditions return.

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    Bacterial Flagella Functions

    Flagella allow bacteria to move towards nutrients (chemotaxis) or away from harmful substances (negative chemotaxis).

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    Study Notes

    Learning Objectives

    • List different types of microorganisms found in foods
    • Compare and contrast cellular characteristics and replication methods of microorganisms
    • Apply microbial naming conventions correctly
    • Differentiate between microbial taxonomy levels
    • Describe characteristics used to categorize bacteria
    • Categorize microorganisms based on growth, survival, and death in foods
    • Differentiate between harmful and non-harmful microorganisms in foods

    Microorganism Characteristics

    Category Cell Type Single/Multicellular Can grow in food?
    Bacteria Prokaryote Single Yes
    Yeast Eukaryote Single Yes
    Mould Eukaryote Multicellular Yes
    Viruses (human/bacteriophages) Acellular None No
    Parasites Eukaryote Single/Multicellular (protozoa/helminths) No

    Study Questions

    • Plasmids: Bacteria can have one or more plasmids. These are small extrachromosomal DNA molecules that carry useful genes, such as resistance to antimicrobial agents.
    • Chromosomes vs. Plasmids: Chromosomes contain essential genes for survival and reproduction, while plasmids carry additional genes providing benefits.

    Bacterial Growth Phases

    • Lag phase: Little to no increase in cell number as bacteria adapt to the environment.
    • Log phase: Exponential growth with rapid cell division.
    • Stationary phase: Growth rate equals death rate due to resource depletion.
    • Death phase: Death rate exceeds growth rate.

    Bacterial Maximum Density

    • The maximum bacterial density in nutritionally rich environments is approximately 9 log CFU/mL (109 CFU/mL).

    Gram-Positive vs. Gram-Negative Bacteria

    • Gram-positive: Thick peptidoglycan layer, retain crystal violet stain (purple).
    • Gram-negative: Thin peptidoglycan layer surrounded by an outer membrane, do not retain crystal violet (pink).

    Temperature Growth for Microorganisms

    • Psychrotrophs: Grow between 0°C and 30°C (optimal around 20-30°C).
    • Mesophiles: Grow between 20°C and 45°C (optimal around 37°C).
    • Psychrophiles: Thrive in extremely cold temperatures (-5°C to 15°C).

    Oxygen Requirements

    • Aerotolerant anaerobes: Do not use oxygen but can survive and grow in its presence.
    • Microaerophiles: Require low levels of oxygen to grow, but are inhibited by higher concentrations.
    • Facultative anaerobes: Grow with or without oxygen.

    Endospores

    • Bacteria form endospores in response to harsh conditions like nutrient depletion, extreme temperatures.
    • Endospores are dormant structures and do not replicate until environmental conditions improve.

    Bacterial Genetic Material Transfer

    • Transformation: Uptake of free DNA from the environment
    • Transduction: Transfer of genetic material via bacteriophages
    • Conjugation: Transfer of plasmids through direct cell-to-cell contact.

    Bacterial Reproduction

    • Binary fission: Asexual reproduction (prokaryotes)
    • Mitosis: Cell division in eukaryotes

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    Lesson 1 Study Guide PDF

    Description

    This quiz covers various types of microorganisms found in foods, their characteristics, and methods of replication. It also delves into microbial taxonomy and highlights the difference between harmful and non-harmful microorganisms. Understanding these concepts is crucial in food safety and microbiology.

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