Microfuge Tube Ren Volume Calculation Quiz

Play an AI-generated podcast conversation about this lesson

Recognition site is a series of nucleotides usually 4 to 8 base pairs long called a ______ site

recognition

A recognition site that reads the same both forwards and backwards is called a ______ sequence

palindromic

Sticky ends leave 3' and 5' ______

overhangs

Blunt ends leave no ______

overhangs Signup and view all the answers

A protein called bovine serum albumin helps stabilize the reaction by preventing the enzyme from sticking to the sides of the container that houses the ______

digest Signup and view all the answers

Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?

ren Signup and view all the answers

10x R.E.buffer 3.Brh (if needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ______ volume

ren Signup and view all the answers

2.mixed by vortexing 3.centrifuge briefly @ 12000 x 6 in a micro centrifuge.to collect contents @ the bottom of the tube 4 incubate @ oprimall temp for R.E.- wually 37°C in a heating block for 1-4 hours s onic digest has completed, incubate rxn mixture @ 65°℃ to heat & activate the K.E.* while KE unt vite specifically (mort of the time) → prolonged incubation times can lead to STAR ACTIVITY cutting sites that are similar but distinct from typical digestion sites 6.Following in activation, DNA is run on, agarare gel - to ensure digest was successful fints sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & incubation temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.

ren Signup and view all the answers

The process of recombining DNA fragments in a process called ______

ligation Signup and view all the answers

If needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume ______

2 Signup and view all the answers

2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume 2.______ by vortexing 3.centrifuge briefly @ 12000 x 6 in a micro centrifuge.to collect contents @ the bottom of the tube 4 incubate @ oprimall temp for R.E.- wually 37°C in a heating block for 1-4 hours s onic digest has completed, incubate rxn mixture @ 65°℃ to heat & activate the K.E.* while KE unt vite specifically (mort of the time) → prolonged incubation times can lead to STAR ACTIVITY cutting sites that are similar but distinct from typical digestion sites 6.Following in activation, DNA is run on, agarare gel - to ensure digest was successful fints sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & incubation temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.

mixed Signup and view all the answers

Sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & ______ temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.

incubation Signup and view all the answers

______ is a series of nucleotides usually 4 to 8 base pairs long called a recognition site

Recognition Signup and view all the answers

A recognition site that reads the same both forwards and backwards is called a ______ sequence

Palindrome Signup and view all the answers

Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?

Steps Signup and view all the answers

Blunt ends leave no ______

Overhang Signup and view all the answers

2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled DNA in 60 minutes @ 31°C in a ______ volume

μL Signup and view all the answers

If needed 4. up to 1 My DNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digest of 1 mg of controlled DNA in 60 minutes @ 31°C in a roμl ren ______

Volume Signup and view all the answers

Filtration separates particulate contents according to size and shape. Membrane filters from intertwined organic filters Polycarbonate filter. Optical Tweezers Optical trapping A focused light beam attracts the medium towards the center of the beam. Isolate cells using ______.

micropipette Signup and view all the answers

Micropipette is used for single cell isolation. It avoids cell damage and enables clean isolation. Phototaxis is used for isolation of flagellates that exhibit ______.

phototaxis Signup and view all the answers

Flow cytometry is used to count and analyze optical properties of cells. It involves of cells. Fluorescence-activated cell (FACS) sorts cells based on specific light scattering and fluorescent characteristics of cells. Isolation and Cultivation Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) involves micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW

sorting Signup and view all the answers

Principle: a focused light source at one end of the apparatus will be used to attract a phototactic cell allowing isolation of cell/s. Flow cytometry To count and analyze optical properties of cells. ______ Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) Principle: micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW

Isolation Signup and view all the answers

H 9926 Office HBW ______

office Signup and view all the answers

Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?

steps Signup and view all the answers

Microfuge Tube Ren Volume Calculation Quiz

Podcast Beta

Questions and Answers

Recognition site is a series of nucleotides usually 4 to 8 base pairs long called a ______ site

A recognition site that reads the same both forwards and backwards is called a ______ sequence

Sticky ends leave 3' and 5' ______

Blunt ends leave no ______

A protein called bovine serum albumin helps stabilize the reaction by preventing the enzyme from sticking to the sides of the container that houses the ______

Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?

10x R.E.buffer 3.Brh (if needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ______ volume

The process of recombining DNA fragments in a process called ______

If needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume ______

Sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & ______ temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.

______ is a series of nucleotides usually 4 to 8 base pairs long called a recognition site

A recognition site that reads the same both forwards and backwards is called a ______ sequence

Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?

Blunt ends leave no ______

2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled DNA in 60 minutes @ 31°C in a ______ volume

If needed 4. up to 1 My DNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digest of 1 mg of controlled DNA in 60 minutes @ 31°C in a roμl ren ______

Filtration separates particulate contents according to size and shape. Membrane filters from intertwined organic filters Polycarbonate filter. Optical Tweezers Optical trapping A focused light beam attracts the medium towards the center of the beam. Isolate cells using ______.

Micropipette is used for single cell isolation. It avoids cell damage and enables clean isolation. Phototaxis is used for isolation of flagellates that exhibit ______.

H 9926 Office HBW ______

Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?

Upgrade to continue