Podcast
Questions and Answers
Recognition site is a series of nucleotides usually 4 to 8 base pairs long called a ______ site
Recognition site is a series of nucleotides usually 4 to 8 base pairs long called a ______ site
recognition
A recognition site that reads the same both forwards and backwards is called a ______ sequence
A recognition site that reads the same both forwards and backwards is called a ______ sequence
palindromic
Sticky ends leave 3' and 5' ______
Sticky ends leave 3' and 5' ______
overhangs
Blunt ends leave no ______
Blunt ends leave no ______
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A protein called bovine serum albumin helps stabilize the reaction by preventing the enzyme from sticking to the sides of the container that houses the ______
A protein called bovine serum albumin helps stabilize the reaction by preventing the enzyme from sticking to the sides of the container that houses the ______
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Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?
Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?
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10x R.E.buffer 3.Brh (if needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ______ volume
10x R.E.buffer 3.Brh (if needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ______ volume
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2.mixed by vortexing 3.centrifuge briefly @ 12000 x 6 in a micro centrifuge.to collect contents @ the bottom of the tube 4 incubate @ oprimall temp for R.E.- wually 37°C in a heating block for 1-4 hours s onic digest has completed, incubate rxn mixture @ 65°℃ to heat & activate the K.E.* while KE unt vite specifically (mort of the time) → prolonged incubation times can lead to STAR ACTIVITY cutting sites that are similar but distinct from typical digestion sites 6.Following in activation, DNA is run on, agarare gel - to ensure digest was successful fints sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & incubation temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.
2.mixed by vortexing 3.centrifuge briefly @ 12000 x 6 in a micro centrifuge.to collect contents @ the bottom of the tube 4 incubate @ oprimall temp for R.E.- wually 37°C in a heating block for 1-4 hours s onic digest has completed, incubate rxn mixture @ 65°℃ to heat & activate the K.E.* while KE unt vite specifically (mort of the time) → prolonged incubation times can lead to STAR ACTIVITY cutting sites that are similar but distinct from typical digestion sites 6.Following in activation, DNA is run on, agarare gel - to ensure digest was successful fints sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & incubation temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.
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The process of recombining DNA fragments in a process called ______
The process of recombining DNA fragments in a process called ______
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If needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume ______
If needed 4.upto 1 My bNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume ______
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2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume 2.______ by vortexing 3.centrifuge briefly @ 12000 x 6 in a micro centrifuge.to collect contents @ the bottom of the tube 4 incubate @ oprimall temp for R.E.- wually 37°C in a heating block for 1-4 hours s onic digest has completed, incubate rxn mixture @ 65°℃ to heat & activate the K.E.* while KE unt vite specifically (mort of the time) → prolonged incubation times can lead to STAR ACTIVITY cutting sites that are similar but distinct from typical digestion sites 6.Following in activation, DNA is run on, agarare gel - to ensure digest was successful fints sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & incubation temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.
2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled ONA in 60 minuter @ 31°C in a roμl ren volume 2.______ by vortexing 3.centrifuge briefly @ 12000 x 6 in a micro centrifuge.to collect contents @ the bottom of the tube 4 incubate @ oprimall temp for R.E.- wually 37°C in a heating block for 1-4 hours s onic digest has completed, incubate rxn mixture @ 65°℃ to heat & activate the K.E.* while KE unt vite specifically (mort of the time) → prolonged incubation times can lead to STAR ACTIVITY cutting sites that are similar but distinct from typical digestion sites 6.Following in activation, DNA is run on, agarare gel - to ensure digest was successful fints sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & incubation temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.
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Sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & ______ temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.
Sometimes, multiple enzymer need to be used to generate a specific DIVA fragment check buffer conditions & ______ temp.if they are compatible bin the 2 enzymes it so, then you can perform double digert & have both enzymes cut @ the same ron sometimes, there's incompatibility in non.conditions.
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______ is a series of nucleotides usually 4 to 8 base pairs long called a recognition site
______ is a series of nucleotides usually 4 to 8 base pairs long called a recognition site
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A recognition site that reads the same both forwards and backwards is called a ______ sequence
A recognition site that reads the same both forwards and backwards is called a ______ sequence
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Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?
Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?
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Blunt ends leave no ______
Blunt ends leave no ______
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2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled DNA in 60 minutes @ 31°C in a ______ volume
2-10 units of enzyme unit = amt of enzyme required to produce a complete digent of 1 mg of controlled DNA in 60 minutes @ 31°C in a ______ volume
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If needed 4. up to 1 My DNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digest of 1 mg of controlled DNA in 60 minutes @ 31°C in a roμl ren ______
If needed 4. up to 1 My DNA 6. 2-10 units of enzyme unit = amt of enzyme required to produce a complete digest of 1 mg of controlled DNA in 60 minutes @ 31°C in a roμl ren ______
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Filtration separates particulate contents according to size and shape. Membrane filters from intertwined organic filters Polycarbonate filter. Optical Tweezers Optical trapping A focused light beam attracts the medium towards the center of the beam. Isolate cells using ______.
Filtration separates particulate contents according to size and shape. Membrane filters from intertwined organic filters Polycarbonate filter. Optical Tweezers Optical trapping A focused light beam attracts the medium towards the center of the beam. Isolate cells using ______.
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Micropipette is used for single cell isolation. It avoids cell damage and enables clean isolation. Phototaxis is used for isolation of flagellates that exhibit ______.
Micropipette is used for single cell isolation. It avoids cell damage and enables clean isolation. Phototaxis is used for isolation of flagellates that exhibit ______.
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Flow cytometry is used to count and analyze optical properties of cells. It involves ______ of cells. Fluorescence-activated cell ______ (FACS) sorts cells based on specific light scattering and fluorescent characteristics of cells. Isolation and Cultivation Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) involves micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW
Flow cytometry is used to count and analyze optical properties of cells. It involves ______ of cells. Fluorescence-activated cell ______ (FACS) sorts cells based on specific light scattering and fluorescent characteristics of cells. Isolation and Cultivation Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) involves micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW
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Principle: a focused light source at one end of the apparatus will be used to attract a phototactic cell allowing isolation of cell/s. Flow cytometry To count and analyze optical properties of cells. ______ Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) Principle: micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW
Principle: a focused light source at one end of the apparatus will be used to attract a phototactic cell allowing isolation of cell/s. Flow cytometry To count and analyze optical properties of cells. ______ Methods for Novel Microorganisms Soil Substrate Membrane System (SSMS) Principle: micro-cultivation of soil bacteria using polycarbonate as growth support and soil extract as substrate. H 9926 Office HBW
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H 9926 Office HBW ______
H 9926 Office HBW ______
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Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?
Microfuge tube → ______ must be followed in order volume of sterile nuclease of water - will yield final van volume of 20 microliter?
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