15 Questions
What does the Gelatin Hydrolysis test determine?
The ability of a bacterium to produce extracellular proteolytic enzymes (gelatinases) that hydrolyze gelatin and liquefy solid gelatin medium.
How is a positive result interpreted in the Gelatin Stab method of the Gelatin Hydrolysis test?
Partial or total liquefaction of the medium.
What does the DNase test differentiate organisms based on?
The production of DNase.
How is a positive result interpreted in the DNase test?
Colorless zone around colonies.
What does the Oxidase test determine?
The presence of the cytochrome oxidase system.
What color change would be observed in the presence of H2S production in the given test?
Black precipitate
What is the outcome of a positive result for Indole in the MIO Agar test?
Pink to red color
What does a RED coloration indicate in the Nitrate Reduction Test?
Reduction of nitrite further to nitrogen gas
What is the significance of inserting a Durham tube upside down in the Nitrate Reduction Test?
Trap the produced nitrogen gas
Which enzyme's production is determined by the MIO Agar test?
Ornithine decarboxylase
What outcome would be expected after adding Zinc Dust if the test has produced a true-negative result for nitrate reduction?
Red coloration
Motile (+) reaction in the given test is indicated by:
Cloudiness spreading from the line of inoculation
What does a positive Ornithine decarboxylase reaction show in the MIO Agar test?
Purple color
In the Nitrate Reduction test, a lack of color development may indicate:
Absence of nitrate reduction (Negative)
What effect does adding α-naphthylamine and sulfanilic acid have in the Nitrate Reduction Test?
Reduces nitrate to nitrite
Test your knowledge on the urease test used in microbiology to determine if a microorganism can hydrolyze urea. Learn about the indicators and reactions associated with the test results.
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