Microbiology Urease Test Quiz
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Questions and Answers

What does the Gelatin Hydrolysis test determine?

The ability of a bacterium to produce extracellular proteolytic enzymes (gelatinases) that hydrolyze gelatin and liquefy solid gelatin medium.

How is a positive result interpreted in the Gelatin Stab method of the Gelatin Hydrolysis test?

Partial or total liquefaction of the medium.

What does the DNase test differentiate organisms based on?

The production of DNase.

How is a positive result interpreted in the DNase test?

<p>Colorless zone around colonies.</p> Signup and view all the answers

What does the Oxidase test determine?

<p>The presence of the cytochrome oxidase system.</p> Signup and view all the answers

What color change would be observed in the presence of H2S production in the given test?

<p>Black precipitate</p> Signup and view all the answers

What is the outcome of a positive result for Indole in the MIO Agar test?

<p>Pink to red color</p> Signup and view all the answers

What does a RED coloration indicate in the Nitrate Reduction Test?

<p>Reduction of nitrite further to nitrogen gas</p> Signup and view all the answers

What is the significance of inserting a Durham tube upside down in the Nitrate Reduction Test?

<p>Trap the produced nitrogen gas</p> Signup and view all the answers

Which enzyme's production is determined by the MIO Agar test?

<p>Ornithine decarboxylase</p> Signup and view all the answers

What outcome would be expected after adding Zinc Dust if the test has produced a true-negative result for nitrate reduction?

<p>Red coloration</p> Signup and view all the answers

Motile (+) reaction in the given test is indicated by:

<p>Cloudiness spreading from the line of inoculation</p> Signup and view all the answers

What does a positive Ornithine decarboxylase reaction show in the MIO Agar test?

<p>Purple color</p> Signup and view all the answers

In the Nitrate Reduction test, a lack of color development may indicate:

<p>Absence of nitrate reduction (Negative)</p> Signup and view all the answers

What effect does adding α-naphthylamine and sulfanilic acid have in the Nitrate Reduction Test?

<p>Reduces nitrate to nitrite</p> Signup and view all the answers

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