Microbiology Techniques Quiz

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What is the main difference between yeast and mold in terms of growth conditions?

Yeast and mold both grow best at 21-25 degrees Celsius.

Yeast grows best at 21-25 degrees Celsius, while mold thrives at 37 degrees Celsius.

Yeast and mold both grow best at 37 degrees Celsius.

Yeast grows best at 37 degrees Celsius, while mold thrives at 21-25 degrees Celsius. (correct)

Which term describes the chain of elongated yeast cells?

Thallus

Pseudohyphae (correct)

Mycelium

Hyphae

What is the purpose of the Aerial part of the mold's Thallus structure?

Obtaining nutrients

Breaking down nutrients

Producing spores

Support reproduction (correct)

What is the difference between Monomorphic and Polymorph fungi?

Monomorphic fungi have one form, while Polymorphic fungi have two distinct forms in their life cycle. (A) Signup and view all the answers

What is the incubation period for fungi cultures before examination in the laboratory?

1 week to several months (D) Signup and view all the answers

Which term refers to the entire body of a mold that is examined under a microscope for identification purposes?

Thallus (A) Signup and view all the answers

What is a characteristic feature of fungi?

Rigid cell walls for maintaining shape and attachment (D) Signup and view all the answers

Why are fungi considered achlorophyllous?

Inability to synthesize their own food (B) Signup and view all the answers

Which characteristic distinguishes fungi from bacteria in terms of habitat?

Prefer natural habitats like soil and organic debris (D) Signup and view all the answers

Why are fungi described as chemotrophic?

They secrete enzymes to degrade organic substrates (A) Signup and view all the answers

Which type of fungi infection does not initiate an immune response due to lack of cellular response?

Superficial infection (A) Signup and view all the answers

What role does the cell wall play in fungi infections?

Maintains shape and facilitates attachment (B) Signup and view all the answers

What staining method is used to detect the presence of Cryptococcus neoformans in cerebrospinal fluid (CSF)?

India ink method (C) Signup and view all the answers

Which type of stain is commonly used to check the presence of intracellular yeast in bone marrow aspirate or peripheral blood smear?

Wright stain (C) Signup and view all the answers

What is the purpose of using Lactophenol Cotton Blue (LPCB) in microscopy for teasing and scotch tape preparations?

To visualize fungal elements (C) Signup and view all the answers

Which staining method is used to identify all fungal elements appearing black?

Gomori's Methenamine Silver Stain (D) Signup and view all the answers

For which purpose is Wood's light typically used in a clinical setting?

Determining prognosis of a patient (D) Signup and view all the answers

Which category of antifungal agents inhibits chitin and DNA synthesis?

Polyoxin compound (B) Signup and view all the answers