Microbiology Techniques Quiz

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18 Questions

What is the main difference between yeast and mold in terms of growth conditions?

Yeast grows best at 37 degrees Celsius, while mold thrives at 21-25 degrees Celsius.

Which term describes the chain of elongated yeast cells?

Pseudohyphae

What is the purpose of the Aerial part of the mold's Thallus structure?

Support reproduction

What is the difference between Monomorphic and Polymorph fungi?

Monomorphic fungi have one form, while Polymorphic fungi have two distinct forms in their life cycle.

What is the incubation period for fungi cultures before examination in the laboratory?

1 week to several months

Which term refers to the entire body of a mold that is examined under a microscope for identification purposes?

Thallus

What is a characteristic feature of fungi?

Rigid cell walls for maintaining shape and attachment

Why are fungi considered achlorophyllous?

Inability to synthesize their own food

Which characteristic distinguishes fungi from bacteria in terms of habitat?

Prefer natural habitats like soil and organic debris

Why are fungi described as chemotrophic?

They secrete enzymes to degrade organic substrates

Which type of fungi infection does not initiate an immune response due to lack of cellular response?

Superficial infection

What role does the cell wall play in fungi infections?

Maintains shape and facilitates attachment

What staining method is used to detect the presence of Cryptococcus neoformans in cerebrospinal fluid (CSF)?

India ink method

Which type of stain is commonly used to check the presence of intracellular yeast in bone marrow aspirate or peripheral blood smear?

Wright stain

What is the purpose of using Lactophenol Cotton Blue (LPCB) in microscopy for teasing and scotch tape preparations?

To visualize fungal elements

Which staining method is used to identify all fungal elements appearing black?

Gomori's Methenamine Silver Stain

For which purpose is Wood's light typically used in a clinical setting?

Determining prognosis of a patient

Which category of antifungal agents inhibits chitin and DNA synthesis?

Polyoxin compound

Test your knowledge on various microbiology techniques such as Gram staining, KOH digestion, Calcofluor white staining, and Giemsa/Wright stain. Learn about the different methods used for detecting bacteria, fungi, and yeast in clinical specimens.

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