Microbiology Study Notes

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Questions and Answers

What is the definition of "spontaneous generation"?

The idea that living organisms can arise from non-living matter.

What is the name given to the process of identifying the cause of a disease?

Etiology

Which of the following is a solidifying agent used in microbiology?

  • Agar (correct)
  • Starch
  • Pectin
  • Gelatin

Viruses are living organisms.

<p>False (B)</p> Signup and view all the answers

Which of the following methods is used to prevent bacterial contamination?

<p>All of the above (D)</p> Signup and view all the answers

Which of the following is NOT a function of the bacterial cell wall?

<p>Protein synthesis (D)</p> Signup and view all the answers

Which of the following is a characteristic of a Gram-positive bacterial cell wall?

<p>Thick layer of peptidoglycan (C)</p> Signup and view all the answers

What is the name of the substance released by gram-negative bacteria when they are destroyed, leading to endotoxic shock?

<p>Lipopolysaccharide (LPS)</p> Signup and view all the answers

What is the name of the structure that helps bacteria move?

<p>Flagella</p> Signup and view all the answers

Which of the following structures is found in both prokaryotic and eukaryotic cells?

<p>Ribosomes (B)</p> Signup and view all the answers

What is the name given to the process by which bacteria pick up DNA from their environment?

<p>Transformation</p> Signup and view all the answers

What is the name of the process by which viruses transfer DNA from one bacterium to another?

<p>Transduction</p> Signup and view all the answers

What is the name of the process by which bacteria exchange DNA through direct contact?

<p>Conjugation</p> Signup and view all the answers

What is the name of the technique used for visualizing the cell wall of bacteria using dyes?

<p>Gram staining</p> Signup and view all the answers

What type of microscope uses UV light to illuminate specimens?

<p>Fluorescent microscope</p> Signup and view all the answers

What type of microscopy uses electrons to create images?

<p>Electron microscopy</p> Signup and view all the answers

What is the name of the process by which larger molecules move across a membrane from a region of high concentration to a region of low concentration?

<p>Diffusion</p> Signup and view all the answers

What is the name of the process by which water moves across a semipermeable membrane from a region of high water concentration to a region of low water concentration?

<p>Osmosis</p> Signup and view all the answers

Which of the following is the primary means of energy production in prokaryotic cells?

<p>Respiration (B)</p> Signup and view all the answers

Fermentation is a more efficient process for energy production than respiration.

<p>False (B)</p> Signup and view all the answers

Which of the following is a common product of fermentation?

<p>All of the above (D)</p> Signup and view all the answers

What is the name of the process by which a bacterial cell takes up DNA from its environment and incorporates it into its own genome?

<p>Transformation</p> Signup and view all the answers

What is the name of the process by which bacteria transfer DNA through direct contact?

<p>Conjugation</p> Signup and view all the answers

Which of the following is a phenotypic characteristic used to identify bacteria?

<p>Morphology (B)</p> Signup and view all the answers

The use of a dichotomous key is a sophisticated technique for identifying microorganisms.

<p>True (A)</p> Signup and view all the answers

What is the name of the outermost layer of a bacterial cell that is composed of carbohydrates, proteins, and sometimes lipids?

<p>Glycocalyx</p> Signup and view all the answers

Bacterial conjugation is a form of sexual reproduction.

<p>False (B)</p> Signup and view all the answers

Flashcards

Protozoa

Single-celled eukaryotes found in moist environments.

Types of Protozoan Movement

Protozoa are classified by movement: pseudopods, cilia, flagella, and apicomplexa.

Cilia

Hair-like projections that help with movement in some protozoa.

Flagella

Longer than cilia, fewer in number; used for movement in some protozoa.

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Apicomplexa

Non-motile protozoa, often parasites causing diseases like malaria.

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Algae

Photosynthetic organisms that can be unicellular or multicellular.

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Agar

Solidifying agent for bacterial culture; has no nutritional value.

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Germ Theory of Disease

The theory that germs (pathogens) cause diseases.

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Koch’s Postulates

Criteria to establish a causative relationship between a microbe and disease.

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Gram Staining

A method to classify bacteria based on their cell wall composition.

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Gram-positive Bacteria

Bacteria with a thick peptidoglycan layer, appearing purple under gram staining.

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Gram-negative Bacteria

Bacteria with a thin peptidoglycan layer, appearing pink under gram staining.

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Endospores

Resistant structures formed by bacteria in response to environmental stress.

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Phagocytosis

Process by which cells engulf and digest particles.

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Mycoplasma

Bacteria that lack cell walls and can cause respiratory infections.

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Active Transport

Movement of molecules against a concentration gradient requiring energy (ATP).

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Passive Transport

Movement of molecules without energy input, following concentration gradients.

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Fermentation

An anaerobic process used by some organisms to convert sugars into energy.

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Endosymbiotic Theory

The theory that eukaryotic cells evolved from symbiotic prokaryotes.

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Nucleus

Membrane-bound organelle that houses DNA in eukaryotic cells.

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Mitochondria

Powerhouse of the cell, produces ATP through respiration.

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Ribosomes

Molecular machines that synthesize proteins in cells.

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Lysosomes

Membrane-bound organelles that contain digestive enzymes.

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Epidemiology

The study of how diseases spread and can be controlled.

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Immunology

The study of the immune system and its response to pathogens.

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Taxonomy

The science of classifying and naming organisms based on shared traits.

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Vertical Gene Transfer

Passing of genetic material from parents to offspring.

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Horizontal Gene Transfer

Transfer of genetic material between organisms of the same generation.

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Microscopy

The use of lenses to magnify objects for study.

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Study Notes

Microbiology Study Notes

  • Microbiology is the study of microscopic organisms.
  • Protozoa are single-celled eukaryotes.
  • They are found in environments with moisture.
  • Protozoa can enter a dormant stage with no water.
  • Protozoa are classified by how they move (pseudopods, cilia, or flagella).
  • Algae can be unicellular or multicellular.
  • They use photosynthesis.
  • Algae are classified by pigmentation.
  • Agar is used to solidify a culture medium for microorganisms.
  • Parasites are organisms that live off and cause damage to a host.
  • Worms, such as flukes and tapeworms, can be parasites.
  • Viruses are non-living infectious particles.
  • They require a host cell to replicate.
  • Spontaneous generation is the false idea that living things arise from non-living matter.
  • Louis Pasteur disproved spontaneous generation using swan-necked flasks.
  • Koch's postulates are used to link a specific microorganism with a specific disease.
  • Etiology is the study of the cause of a disease.
  • Pathogens are disease-causing agents.
  • Aseptic techniques and handwashing prevent contamination in scientific procedures.
  • Microbes can cause serious disease in humans, animals and plants.
  • Microbial life on earth is vital for the environment.
  • Antibiotics are used to treat bacterial infections.
  • Microbial life affects many human activities.
  • Bacteria, Archaea, and Eukarya are domains in the tree of life.
  • Taxonomy involves categorizing and classifying organisms.
  • Microbial classification is a critical part of Microbiology.
  • Microscopy, staining, and classification methods are critical for studying microbes.
  • Different microscopy techniques exist (light, electron).
  • Staining methods facilitate visualization of microbes.
  • Various staining techniques emphasize different aspects of microbes.
  • Bacteria reproduce through binary fission.
  • Microbial genetics involves studying the inheritance and transfer of genetic material.
  • Bacteria can swap genes (horizontal transfer).
  • Transformation involves taking up external DNA.
  • Transduction utilizes viruses to transfer genetic material.
  • Conjugation uses pili for cell-to-cell contact and gene transfer.
  • Microbial metabolism includes various pathways (Glycolysis, Krebs Cycle).
  • Fermentation can occur when oxygen is scarce and involves other electron acceptors.
  • Microbial life and infection can have both positive and negative impacts on ecosystems and human life.

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