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Questions and Answers
What is true about Streptococci and Enterococci?
What is true about Streptococci and Enterococci?
What is Beta Hemolysis?
What is Beta Hemolysis?
Complete lysis of RBCs around a colony, resulting in a clear zone surrounding the colonies.
What is Alpha Hemolysis?
What is Alpha Hemolysis?
Partial breakdown of the hemoglobin inside RBCs, producing a green discoloration around the colonies.
Define Gamma Hemolysis.
Define Gamma Hemolysis.
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What is the significance of Group A Streptococci?
What is the significance of Group A Streptococci?
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What infections are associated with Group B Streptococci?
What infections are associated with Group B Streptococci?
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What are the characteristics of Alpha Hemolytic Group: Streptococcus pneumoniae?
What are the characteristics of Alpha Hemolytic Group: Streptococcus pneumoniae?
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What is the function of the CAMP test?
What is the function of the CAMP test?
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What distinguishes humans as carriers of Staphylococci and Streptococci?
What distinguishes humans as carriers of Staphylococci and Streptococci?
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What is the appearance difference between S.agalactiae and S.pyrogenes on blood agar?
What is the appearance difference between S.agalactiae and S.pyrogenes on blood agar?
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What streptococcal species is investigated in third trimester pregnant women?
What streptococcal species is investigated in third trimester pregnant women?
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Which streptococci are implicated in dental caries?
Which streptococci are implicated in dental caries?
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Study Notes
Streptococci & Enterococci Overview
- Streptococci and enterococci are primarily found in chains and are Gram-positive bacteria.
- They are facultative anaerobes, nonmotile, and lack catalase.
Hemolysis Types
- Beta Hemolysis: Complete lysis of red blood cells (RBCs) creates a clear zone around colonies.
- Alpha Hemolysis: Partial breakdown of hemoglobin in RBCs results in a green discoloration around colonies.
- Gamma Hemolysis: No hemolysis occurs, with no effect on RBCs.
Beta Hemolytic Groups
- Group A Streptococci (S. pyrogenes): Most serious, primarily found in the pharynx, skin, and rectum; produces hemolysins causing complete lysis of RBCs.
- Group B Streptococci (S. agalactiae): Common in the genital and intestinal tracts; significant neonatal infections including sepsis and meningitis. Identified by positive CAMP reaction.
- Group C Streptococci (S. dysgalactiae): Uncommon pathogens; involved in pharyngitis and endocarditis, differentiated by Bacitracin resistance and negative CAMP test.
Alpha Hemolytic Groups
- Streptococcus pneumoniae: Causes bacterial pneumonia, meningitis, and otitis media; virulent strains have a polysaccharide capsule and are positive for optochin susceptibility.
- Viridans Streptococci: Opportunistic infections, associated with dental caries; appear small and grayish on blood agar, with negative bile esculin and variable salt tolerance test results.
Enterococci Groups
- Group D Enterococci (E. faecalis and E. faecium): Inhabit the GI tract and oral cavity; can cause urinary tract infections, bacteremia, and endocarditis. Show large non-hemolytic colonies.
- Group D Nonenterococci (S. bovis): Present in the intestinal tract; associated with GI malignancies and can cause endocarditis.
Sensitivity Tests
- Optochin Sensitivity: Pneumococci are sensitive; viridans are resistant.
- Bacitracin Sensitivity: S. pyrogenes is sensitive, while S. agalactiae is resistant.
- SXT Sensitivity: Useful for identifying group C streptococci.
Identification Tests
- CAMP Test: Positive for S. agalactiae, indicating enhanced breakdown of RBCs.
- Lancefield Antigen Testing: Differentiates between streptococcal groups based on carbohydrate antigens.
- NaCl Tolerance Test: Distinguishes enterococci from other group D streptococci.
- SXT Test: Group A strep is resistant, while group C is sensitive.
Clinical Applications
- Throat Swab on Blood Agar: Stabbing creates anaerobic conditions, improving hemolysis development.
- Pregnant Women Screening: Focus on S. agalactiae (group B strep) status during the third trimester.
- Dental Caries Development: Caused by viridans streptococci like S. mutans fermenting sugars and producing enamel-eroding acids.
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Description
Test your knowledge on Streptococci and Enterococci with this informative quiz designed specifically for microbiology students. Covering important concepts such as hemolysis and characteristics of these bacteria, this quiz helps reinforce your understanding of gram-positive microorganisms.