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Questions and Answers
What is a standard plate count?
What is a standard plate count?
It is an estimate of microorganisms in a sample.
What is the incubation time for the Standard Plate Count?
What is the incubation time for the Standard Plate Count?
What temperature should the media be when poured for the Standard Plate Count?
What temperature should the media be when poured for the Standard Plate Count?
30 ± 1 °C
What is the difference between selective and non-selective media?
What is the difference between selective and non-selective media?
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What is the maximum stacking height of plates?
What is the maximum stacking height of plates?
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Plates should be bagged after incubation.
Plates should be bagged after incubation.
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What is the name of the agar used for the Standard Plate Count?
What is the name of the agar used for the Standard Plate Count?
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What type of bacteria can grow at temperatures less than or equal to 7 °C?
What type of bacteria can grow at temperatures less than or equal to 7 °C?
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Study Notes
Media Background
- Standard Plate Count (SPC) is used to estimate the number of microorganisms in a sample.
- Milk Plate Count Agar (MPCA) is a non-selective medium used for SPC, allowing for a broad range of organisms to grow.
- Plates intended for SPC should be inverted to prevent condensation.
Incubation Time and Temperature
- Incubation time for SPC is 72 ± 3 hours at a temperature of 30 ± 1 °C.
- Psychrotroph bacteria can grow at temperatures of 7 °C or lower, with an incubation period of up to 10 days.
Maximum Stacking Height
- A maximum of 12 plates can be stacked during incubation.
Target Organism and Organism Background
- Target organisms for SPC include a wide variety of bacteria, particularly in foods like milk.
Miscellaneous Information
- Selective media contain specific properties to favor the growth of certain microorganisms, while non-selective media allow various organisms to grow without targeting any specific one.
- Standard Methods Agar (SMA) is another example of non-selective media, facilitating diverse organism growth when using pour plate technique.
Additional Procedures
- Plates for psychrotrophic testing must also be inverted to minimize water accumulation and not bagged to prioritize airflow during incubation.
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Description
Test your knowledge on Standard Plate Count (SPC) techniques, including the use of Milk Plate Count Agar (MPCA). This quiz covers incubation conditions, maximum stacking limits, and the types of microorganisms that can be detected. Ideal for students studying food microbiology and laboratory procedures.
