Microbiology Smear Preparation for Staining
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Questions and Answers

What is the purpose of fixing microorganisms to a microscope slide before staining?

  • To enhance the color of the stain
  • To prevent the stain from washing the microbes off the slide (correct)
  • To speed up the staining process
  • To make the microorganisms easier to observe under the microscope

What is a smear in microbiology staining?

  • A type of stain used in negative staining
  • A film of material containing microorganisms spread on a slide (correct)
  • An acidic solution used to wash off excess stain
  • A dye used in basic staining techniques

Which type of dye is crystal violet?

  • Basic dye (correct)
  • Acidic dye
  • Neutral dye
  • Contrast dye

Why are basic dyes attracted to bacterial cells?

<p>Because most bacterial surfaces are positively charged (D)</p> Signup and view all the answers

What is negative staining in microbiology?

<p>A method where colorless bacteria are contrasted against a colored background (C)</p> Signup and view all the answers

Which type of stain consists of salts composed of a positive and negative ion?

<p>Basic stain (B)</p> Signup and view all the answers

What contributes to making an enlarged and inverted image when using a microscope?

<p>Light refraction (A)</p> Signup and view all the answers

In darkfield microscopy, what is the result of having no background light?

<p>Specimen appears light against a black background (B)</p> Signup and view all the answers

What does a compound light microscope use as its source of illumination?

<p>Visible light (C)</p> Signup and view all the answers

Why is darkfield microscopy used for examining certain specimens?

<p>Specimens are invisible in ordinary light microscopes (C)</p> Signup and view all the answers

Which microscope is commonly used in many laboratories and schools?

<p>Compound light microscope (C)</p> Signup and view all the answers

What type of microscope uses two lenses to magnify images?

<p>Microscopes in general (A)</p> Signup and view all the answers

What is the main advantage of Phase-Contrast Microscopy for examining living microorganisms?

<p>Sharper definition of internal structures (A)</p> Signup and view all the answers

How does Phase-Contrast Microscopy create an image of the specimen?

<p>By bringing together light rays from different sources (A)</p> Signup and view all the answers

What distinguishes Differential Interference Contrast (DIC) Microscopy from Phase-Contrast Microscopy?

<p>Higher resolution (C)</p> Signup and view all the answers

What is the primary advantage of Fluorescence Microscopy in observing specimens?

<p>Creation of luminescent objects (C)</p> Signup and view all the answers

When is a fluorochrome stain used in Fluorescence Microscopy?

<p>When the specimen does not naturally fluoresce (C)</p> Signup and view all the answers

Which microscopy method provides images that are brightly colored and nearly three-dimensional?

<p>Fluorescence Microscopy (C)</p> Signup and view all the answers

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