18 Questions
What is the purpose of fixing microorganisms to a microscope slide before staining?
To prevent the stain from washing the microbes off the slide
What is a smear in microbiology staining?
A film of material containing microorganisms spread on a slide
Which type of dye is crystal violet?
Basic dye
Why are basic dyes attracted to bacterial cells?
Because most bacterial surfaces are positively charged
What is negative staining in microbiology?
A method where colorless bacteria are contrasted against a colored background
Which type of stain consists of salts composed of a positive and negative ion?
Basic stain
What contributes to making an enlarged and inverted image when using a microscope?
Light refraction
In darkfield microscopy, what is the result of having no background light?
Specimen appears light against a black background
What does a compound light microscope use as its source of illumination?
Visible light
Why is darkfield microscopy used for examining certain specimens?
Specimens are invisible in ordinary light microscopes
Which microscope is commonly used in many laboratories and schools?
Compound light microscope
What type of microscope uses two lenses to magnify images?
Microscopes in general
What is the main advantage of Phase-Contrast Microscopy for examining living microorganisms?
Sharper definition of internal structures
How does Phase-Contrast Microscopy create an image of the specimen?
By bringing together light rays from different sources
What distinguishes Differential Interference Contrast (DIC) Microscopy from Phase-Contrast Microscopy?
Higher resolution
What is the primary advantage of Fluorescence Microscopy in observing specimens?
Creation of luminescent objects
When is a fluorochrome stain used in Fluorescence Microscopy?
When the specimen does not naturally fluoresce
Which microscopy method provides images that are brightly colored and nearly three-dimensional?
Fluorescence Microscopy
Learn about the process of preparing smears for staining in microbiology, which involves fixing microorganisms to a microscope slide before applying dye. Understand the steps of creating a smear, staining, and washing off excess dye.
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