Microbiology Smear Preparation for Staining
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Questions and Answers

What is the purpose of fixing microorganisms to a microscope slide before staining?

  • To enhance the color of the stain
  • To prevent the stain from washing the microbes off the slide (correct)
  • To speed up the staining process
  • To make the microorganisms easier to observe under the microscope

    • What is a smear in microbiology staining?

  • A type of stain used in negative staining
  • A film of material containing microorganisms spread on a slide (correct)
  • An acidic solution used to wash off excess stain
  • A dye used in basic staining techniques

    • Which type of dye is crystal violet?

  • Basic dye (correct)
  • Acidic dye
  • Neutral dye
  • Contrast dye

    • Why are basic dyes attracted to bacterial cells?

    <p>Because most bacterial surfaces are positively charged</p> Signup and view all the answers

    What is negative staining in microbiology?

    <p>A method where colorless bacteria are contrasted against a colored background</p> Signup and view all the answers

    Which type of stain consists of salts composed of a positive and negative ion?

    <p>Basic stain</p> Signup and view all the answers

    What contributes to making an enlarged and inverted image when using a microscope?

    <p>Light refraction</p> Signup and view all the answers

    In darkfield microscopy, what is the result of having no background light?

    <p>Specimen appears light against a black background</p> Signup and view all the answers

    What does a compound light microscope use as its source of illumination?

    <p>Visible light</p> Signup and view all the answers

    Why is darkfield microscopy used for examining certain specimens?

    <p>Specimens are invisible in ordinary light microscopes</p> Signup and view all the answers

    Which microscope is commonly used in many laboratories and schools?

    <p>Compound light microscope</p> Signup and view all the answers

    What type of microscope uses two lenses to magnify images?

    <p>Microscopes in general</p> Signup and view all the answers

    What is the main advantage of Phase-Contrast Microscopy for examining living microorganisms?

    <p>Sharper definition of internal structures</p> Signup and view all the answers

    How does Phase-Contrast Microscopy create an image of the specimen?

    <p>By bringing together light rays from different sources</p> Signup and view all the answers

    What distinguishes Differential Interference Contrast (DIC) Microscopy from Phase-Contrast Microscopy?

    <p>Higher resolution</p> Signup and view all the answers

    What is the primary advantage of Fluorescence Microscopy in observing specimens?

    <p>Creation of luminescent objects</p> Signup and view all the answers

    When is a fluorochrome stain used in Fluorescence Microscopy?

    <p>When the specimen does not naturally fluoresce</p> Signup and view all the answers

    Which microscopy method provides images that are brightly colored and nearly three-dimensional?

    <p>Fluorescence Microscopy</p> Signup and view all the answers

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