Microbiology Quiz: Bacteria and Disinfection

Multiple Choice Questions (Section A)

• Diplococci is a classification of Bacteria
• Bacteria remain constant in the Log Phase of their growth cycle.
• A type of electron microscope is TEM (transmission electron microscope) and SEM (scanning electron microscope).
• The suitable method for sterilizing rubber is Hot air oven, Autoclave, or Filtration.
• Staining is used for the purpose of improving visibility of microorganisms.

Identification of Microorganisms (Section A)

• Identification of microorganisms is a method for determining the type of microorganism.
• Sterilization of microorganism is a method to kill microorganisms, to make them sterile.
• Cultivation of microorganisms is done in pure cultures to study or isolate them for various purposes.

Mechanism of Disinfectant Action (Section A)

• Alcohol acts as a disinfectant by precipitating cell of bacteria and increasing the permeability of water within the cells.

Disinfection Conditions (Section A)

• Factors that affect disinfectants efficacy include organic matter, temperature, and concentration.

Microorganism Killing Agent (Section A)

• An agent that kills microorganisms (bacteria) is called a bactericidal agent.

Antibiotic Assay (Section A)

• Assay method for antibiotics includes Cup-plate method, Serial dilution method, and Disc-plate method.

Vitamin Assay (Section A)

• Purpose of vitamin assay is studying the growth or death of bacteria in the presence of vitamin.

BOD Definition (Section A)

• BOD stands for Biological Oxygen Demand.

Food Preservation Methods (Section A)

• Substances like oil, salt, and canning are used in food preservation techniques.

Transferring Microorganisms (Section A)

• Transferring microorganisms from cultures to create a desired culture is called a pure culture technique.

Penicillin Discovery (Section A)

• Alexander Fleming discovered penicillin.

Recombinant DNA Technology (Section A)

• Penicillin and Ampicillin are examples of drugs made using recombinant DNA technology.

Fermentation Technique (Section A)

• Louis Pasteur is associated with the fermentation technique.

Virology (Section A)

• The study of viruses is called Virology.

Staining Techniques (Section A)

• Negative staining, Acid fast, and Gram staining are various staining techniques.

Microbiology Father (Section A)

• Robert Koch is considered the father of microbiology.

Cell Culture (Section B/C)

• Inoculation, Culture media, and pure culture are components of cell culture.

Sterilization Methods (Section B)

• Various sterilization methods involve classifying and explaining each method, considering advantages, and limitations.

Microbial Assay Method (Section B)

• Cup-plate and serial dilution methods are used for microbial assay.

Staining (Section B)

• Stain types include how they differ in how they are used and perceived during staining. The detailed method Gram staining is explained.

Louis Pasteur's Contributions (Section C)

• Louis Pasteur contributed microbiological examples.

Bacterial Classification (Section C)

• Ways to classify bacteria and criteria used.

Disinfection Factors (Section C)

• Factors impacting the efficacy of disinfection.

Pharmaceutical Industry Application of Cell Cultures (Section C)

• Applications of cell cultures in the pharmaceutical field.

Pharmaceutical Product Preservation (Section C)

• Techniques for preserving pharmaceutical products.

Bacterial Growth Cycle (Section C)

• Details on the various phases of bacterial growth.

Electron Microscope Parts (Section C)

• Elaboration on parts and functions of electron microscopes.

Aseptic Area Contamination (Section C)

• Potential sources of contamination in aseptic areas.

BOD and COD (Section C)

• Explanation of BOD and COD in environmental cleanliness.