Microbiology: Pour Plate Method Flashcards

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the pour plate method?

A method of inoculating a solid nutrient medium by mixing bacteria in the melted medium and pouring the medium into a Petri dish to solidify.

What materials are required for the pour plate method?

Alcohol lamp, cotton swab applicator, lighter, masking tape, nutrient agar, sharpie, sterile petri dish.

What is the first step in the pour plate method?

Obtain a sterile petri dish and do not open it.

What is the second step in the pour plate method?

Light the alcohol lamp with a lighter. Signup and view all the answers

What should you do in step 3 of the pour plate method?

Sterilize the bottle of nutrient agar by passing the rim of the bottle and the cap through the flame of the lit alcohol lamp. Signup and view all the answers

What is step 4 of the pour plate method?

Pour the melted nutrient agar into the petri dish. Signup and view all the answers

What is the purpose of sterilizing the bottle and cap after pouring the agar?

To prevent contamination of the agar. Signup and view all the answers

What is step 6 in the pour plate method?

Collect swab samples while or after the nutrient agar solidifies. Signup and view all the answers

What should be done once the nutrient agar solidifies?

Apply the sample gently onto it by moving the cotton swab applicator back and forth on the surface. Signup and view all the answers

What should you label on the masking tape in step 8?

The date, your initials, the type of agar used, and the location of the sample. Signup and view all the answers

What do you do with the labeled masking tape?

Place it on the petri dish. Signup and view all the answers

At what temperature should the sampled petri dish be stored?

37 degrees Celsius. Signup and view all the answers

What should you do after obtaining the petri dish from the incubator?

Observe the colonies in your petri dish. Signup and view all the answers

What should be done with the petri dish after observing the colonies?

Discard the dish in the Biohazard Bin/Container. Signup and view all the answers

What is the purpose of the pour plate method?

To count the number of colony-forming bacteria in a sample. Signup and view all the answers

Study Notes

Pour Plate Method Overview

A technique for inoculating solid nutrient medium by mixing bacteria with melted agar.
The mixture is poured into a Petri dish where it solidifies and is incubated for colony growth.

Required Materials

Alcohol lamp
Cotton swab applicator
Lighter
Masking tape
Nutrient agar (NA)
Sharpie
Sterile Petri dish

Step-by-Step Procedure

Obtain and keep a sterile Petri dish sealed to prevent contamination.
Ignite the alcohol lamp using a lighter for sterilization.
Sterilize the rim and cap of the nutrient agar bottle by passing through the alcohol flame.
Pour melted nutrient agar into the sterile Petri dish.
After pouring, sterilize the agar bottle and cap again with the alcohol flame.
Collect swab samples while the agar solidifies.
Once solidified, gently apply the sample onto the agar with the cotton swab in a back-and-forth motion.
Label masking tape with the sample date, initials, type of agar, and sample location.
Affix the labeled tape onto the lid of the Petri dish.
Incubate the labeled Petri dish at 37°C for optimal bacterial growth.
Retrieve the Petri dish post-incubation for observation.
Examine the dish for bacterial colonies.
Discard the Petri dish in a Biohazard Bin/Container after observations are completed.

Purpose of the Pour Plate Method

This method allows for the quantification of colony-forming units (CFUs) in a sample for microbial analysis.

Studying That Suits You

Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

Description

Study key concepts and terms related to the pour plate method in microbiology. These flashcards provide definitions and a list of materials required for the technique, making it an effective tool for learning. Perfect for students preparing for exams or seeking to reinforce their understanding of microbiological methods.