Questions and Answers
What is the pour plate method?
A method of inoculating a solid nutrient medium by mixing bacteria in the melted medium and pouring the medium into a Petri dish to solidify.
What materials are required for the pour plate method?
Alcohol lamp, cotton swab applicator, lighter, masking tape, nutrient agar, sharpie, sterile petri dish.
What is the first step in the pour plate method?
Obtain a sterile petri dish and do not open it.
What is the second step in the pour plate method?
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What should you do in step 3 of the pour plate method?
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What is step 4 of the pour plate method?
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What is the purpose of sterilizing the bottle and cap after pouring the agar?
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What is step 6 in the pour plate method?
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What should be done once the nutrient agar solidifies?
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What should you label on the masking tape in step 8?
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What do you do with the labeled masking tape?
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At what temperature should the sampled petri dish be stored?
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What should you do after obtaining the petri dish from the incubator?
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What should be done with the petri dish after observing the colonies?
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What is the purpose of the pour plate method?
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Study Notes
Pour Plate Method Overview
- A technique for inoculating solid nutrient medium by mixing bacteria with melted agar.
- The mixture is poured into a Petri dish where it solidifies and is incubated for colony growth.
Required Materials
- Alcohol lamp
- Cotton swab applicator
- Lighter
- Masking tape
- Nutrient agar (NA)
- Sharpie
- Sterile Petri dish
Step-by-Step Procedure
- Obtain and keep a sterile Petri dish sealed to prevent contamination.
- Ignite the alcohol lamp using a lighter for sterilization.
- Sterilize the rim and cap of the nutrient agar bottle by passing through the alcohol flame.
- Pour melted nutrient agar into the sterile Petri dish.
- After pouring, sterilize the agar bottle and cap again with the alcohol flame.
- Collect swab samples while the agar solidifies.
- Once solidified, gently apply the sample onto the agar with the cotton swab in a back-and-forth motion.
- Label masking tape with the sample date, initials, type of agar, and sample location.
- Affix the labeled tape onto the lid of the Petri dish.
- Incubate the labeled Petri dish at 37°C for optimal bacterial growth.
- Retrieve the Petri dish post-incubation for observation.
- Examine the dish for bacterial colonies.
- Discard the Petri dish in a Biohazard Bin/Container after observations are completed.
Purpose of the Pour Plate Method
- This method allows for the quantification of colony-forming units (CFUs) in a sample for microbial analysis.
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Study key concepts and terms related to the pour plate method in microbiology. These flashcards provide definitions and a list of materials required for the technique, making it an effective tool for learning. Perfect for students preparing for exams or seeking to reinforce their understanding of microbiological methods.
