Microbiology Overview and Classification
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Questions and Answers

Which of the following structures is NOT found in all bacterial cells?

  • Capsule (correct)
  • Ribosomes
  • Plasma membrane
  • Cell wall
  • What is the primary function of pili in bacterial cells?

  • Movement
  • DNA transfer (correct)
  • Protection from environmental stressors
  • Adhesion to surfaces
  • Which of the following structures is NOT directly involved in bacterial motility?

  • Endospores (correct)
  • Flagella
  • Pili
  • Fimbriae
  • What is the major supporting structure in the cytoplasm of a bacterium?

    <p>Cytoskeleton (A)</p> Signup and view all the answers

    What evidence supports the theory of endosymbiosis?

    <p>Mitochondria and chloroplasts have their own DNA and ribosomes. (A)</p> Signup and view all the answers

    Which of the following bacterial structures is directly involved in resisting phagocytosis by immune cells?

    <p>Capsule (D)</p> Signup and view all the answers

    What is a defining feature of prokaryotic cells?

    <p>Absence of a nucleus (C)</p> Signup and view all the answers

    Which of the following shapes describes a bacterium that is rod-shaped?

    <p>Bacilli (D)</p> Signup and view all the answers

    What term is used to describe bacterial cells arranged in clusters?

    <p>Staphylo (B)</p> Signup and view all the answers

    Which type of transport in bacteria requires energy to move substances against their concentration gradient?

    <p>Active transport (D)</p> Signup and view all the answers

    What structural component provides bacterial cells with their shape and protects them from osmotic shock?

    <p>Peptidoglycan (C)</p> Signup and view all the answers

    Which of the following best describes the transport of small, uncharged polar molecules across bacterial membranes?

    <p>Somewhat permeable (A)</p> Signup and view all the answers

    When comparing bacteria and eukaryotic cells, what differentiates eukaryotes in terms of transport mechanisms?

    <p>Endocytosis and exocytosis (C)</p> Signup and view all the answers

    What characteristic of Gram-negative bacteria distinguishes them from Gram-positive bacteria?

    <p>Presence of outer membrane (C)</p> Signup and view all the answers

    Which of the following is NOT a correct description of cocci bacteria?

    <p>They are often rod-shaped. (A)</p> Signup and view all the answers

    What are the main inputs for the Calvin cycle?

    <p>CO2, ATP, NADPH (B)</p> Signup and view all the answers

    Which of the following outputs is produced by the Calvin cycle?

    <p>Glucose (B)</p> Signup and view all the answers

    Which of the following statements about photosynthesis is true?

    <p>O2 is produced during photosystem II reactions. (A)</p> Signup and view all the answers

    What type of organism can utilize organic compounds as a carbon source and uses light for energy?

    <p>Phototroph (A)</p> Signup and view all the answers

    Identifying the classification of microbes, which of the following best describes obligate aerobes?

    <p>Organisms that require oxygen to grow. (D)</p> Signup and view all the answers

    Which type of microbial media contains undefined compositions often used for culturing fastidious organisms?

    <p>Complex media (C)</p> Signup and view all the answers

    In the nitrogen cycle, which process refers to the conversion of atmospheric nitrogen into a usable form for plants?

    <p>Nitrogen fixation (D)</p> Signup and view all the answers

    What term describes the time it takes for a population to double in size during exponential growth?

    <p>Doubling time (C)</p> Signup and view all the answers

    Which of the following statements about cytochrome oxidase is true?

    <p>Some prokaryotes utilize ubiquinol oxidase instead of cytochrome c oxidase. (A)</p> Signup and view all the answers

    What is the primary function of pili in prokaryotes?

    <p>Aid in adherence to surfaces (D)</p> Signup and view all the answers

    What is the role of light in phototrophs' energy generation?

    <p>It powers ATP and NADPH production through photoexcitation. (A)</p> Signup and view all the answers

    Which of the following correctly defines a chemolithotroph?

    <p>An organism that obtains energy from inorganic compounds. (C)</p> Signup and view all the answers

    Which type of flagella arrangement is described as having flagella located at one or both ends?

    <p>Polar (D)</p> Signup and view all the answers

    What distinguishes oxygenic phototrophs from anoxygenic phototrophs?

    <p>Oxygenic phototrophs produce oxygen while anoxygenic phototrophs do not. (C)</p> Signup and view all the answers

    What distinguishes prokaryotic flagella from eukaryotic flagella?

    <p>Prokaryotic flagella use proton motive force for movement (D)</p> Signup and view all the answers

    How do lithotrophs primarily harvest energy from their environment?

    <p>By oxidizing inorganic molecules. (C)</p> Signup and view all the answers

    What is the structure of the glycocalyx found in prokaryotes?

    <p>A sugar shell made mostly of polysaccharides (D)</p> Signup and view all the answers

    What is the defining characteristic of chemosynthesis compared to photosynthesis?

    <p>Chemosynthesis does not produce oxygen. (D)</p> Signup and view all the answers

    What role do S layers play in some bacteria?

    <p>Strengthen the cell wall (D)</p> Signup and view all the answers

    What is one way autotrophic microbes fix CO2 into biomass?

    <p>By incorporating inorganic carbon into organic compounds using ATP. (C)</p> Signup and view all the answers

    How does chemotaxis function in prokaryotic cells?

    <p>By rotating the flagella to respond to stimuli (B)</p> Signup and view all the answers

    What characterizes the slime layer of the glycocalyx?

    <p>Unorganized, soft, and loose (A)</p> Signup and view all the answers

    Which of the following describes how phototrophs generate ATP?

    <p>Via light-activated electron transport chain processes. (B)</p> Signup and view all the answers

    What is the method by which prokaryotic cells primarily obtain energy for flagellar movement?

    <p>Proton motive force (C)</p> Signup and view all the answers

    What is the primary function of pili in E. coli?

    <p>Attachment to surfaces (C)</p> Signup and view all the answers

    Which statement accurately differentiates efflux from secretion in bacterial cells?

    <p>Efflux is the removal of toxins, while secretion refers to the release of proteins. (D)</p> Signup and view all the answers

    Which of the following is a characteristic of the outer membrane of Gram-negative bacteria?

    <p>Contains porins and LPS (B)</p> Signup and view all the answers

    What is the primary product of glycolysis from one molecule of glucose?

    <p>2 ATP, 2 NADH, and 2 pyruvate (D)</p> Signup and view all the answers

    What differentiates the Entner-Doudoroff pathway from glycolysis?

    <p>It specifically uses sugar acids with carboxyl groups. (C)</p> Signup and view all the answers

    Which of the following is primarily generated through the Pentose Phosphate pathway?

    <p>NADPH and ribose-5-phosphate (C)</p> Signup and view all the answers

    In what conditions does pyruvate undergo catabolism through the TCA cycle?

    <p>When oxygen is available (D)</p> Signup and view all the answers

    What is the main purpose of catabolism in cellular metabolism?

    <p>To convert complex food molecules into simpler catabolites for energy (B)</p> Signup and view all the answers

    Flashcards

    Bacilli

    Rod-shaped bacteria that are typically long and straight.

    Strepto-

    Prefix meaning 'chains' in bacterial arrangements.

    Cocci

    Spherical-shaped bacteria that can exist individually or in clusters.

    Passive transport

    Movement of nutrients along the concentration gradient without energy use.

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    Peptidoglycan

    A structural component of bacterial cell walls made of glycan chains and peptides.

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    Gram-positive vs. Gram-negative

    Two types of bacterial cell walls differing in thickness and stain response.

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    Spirochetes

    Long, flexible bacteria with a corkscrew shape, equipped with axial filaments.

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    Active transport

    Movement of substances against the concentration gradient, requiring energy.

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    Cell Envelope

    The protective layer surrounding bacterial cells, includes plasma membrane, cell wall, and outer membrane.

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    Cytoplasm

    A gel-like substance where proteins and macromolecules are suspended within the bacterial cell.

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    Nucleoid Region

    Area in prokaryotic cells containing DNA, including chromosomal DNA and plasmids.

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    Capsule

    A protective polysaccharide or protein layer outside the cell wall that aids in adhesion and protects from phagocytosis.

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    Flagella

    Whip-like appendages that aid in bacterial movement.

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    Endospores

    Resistant structures formed by some bacteria that protect against harsh conditions.

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    Quorum Sensing

    Cell-to-cell communication mechanism using signaling molecules to coordinate group behaviors.

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    Endosymbiosis

    Evolutionary process where larger cells engulfed smaller bacteria, leading to organelle formation.

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    Pili

    Hair-like structures for adherence and sometimes motility.

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    Chemotaxis

    Movement of a cell in response to chemical stimuli.

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    Flagellar motility

    Movement of prokaryotes using flagella for propulsion.

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    Prokaryotic flagella

    Made of flagellin, spins, powered by proton motive force.

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    Eukaryotic flagella

    Made of microtubules, whip-like motion using ATP.

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    Glycocalyx

    Sugar shell protecting cells, includes capsules and slime layers.

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    S Layer

    Protein or glycoprotein lattice outside the cell wall in some bacteria.

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    Anaerobic respiration

    A form of respiration that occurs without oxygen, using alternative electron acceptors.

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    Chemotroph

    Organisms that obtain energy by oxidizing chemical compounds.

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    Carbon fixation

    The process of converting inorganic CO2 into organic compounds by autotrophic organisms.

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    Lithotrophs

    Organisms that obtain energy from inorganic compounds.

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    Photosystem II

    Part of photosynthesis, it uses light to split water molecules to release oxygen.

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    Oxygenic vs Anoxygenic phototrophs

    Oxygenic phototrophs use water and produce oxygen, while anoxygenic phototrophs do not produce oxygen and may use sulfur.

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    Autotroph

    Organisms that create their own food from inorganic substances, usually CO2.

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    Chemoautotroph

    Organisms that obtain energy from chemical compounds and use CO2 as a carbon source.

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    Biofilms

    Thin layers of bacteria that form on surfaces, especially problematic in lungs.

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    Cell wall function

    Provides shape and protects bacteria from osmotic stress.

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    ATP and NADH

    Energy carriers that transfer energy between catabolic and anabolic reactions.

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    Glycolysis

    Process that breaks down glucose to produce ATP and pyruvate.

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    Entner-Doudoroff pathway

    A metabolic pathway yielding NADPH, ATP, and pyruvic acid using sugar acids.

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    Pentose Phosphate pathway

    A pathway that mainly produces NADPH and ribose-5-phosphate, for biosynthesis.

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    Pyruvate catabolism

    Conversion of pyruvate during fermentation or in the TCA cycle.

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    Calvin Cycle

    Cycle that converts CO2 into glucose using ATP and NADPH.

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    Calvin Cycle Inputs

    Essential components needed for the Calvin cycle: CO2, ATP, NADPH.

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    Calvin Cycle Outputs

    Products of the Calvin cycle: glucose, ADP, NADP+.

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    Aerobes vs Anaerobes

    Aerobes need oxygen, anaerobes do not.

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    Fastidious Organisms

    Organisms that require specific nutritional factors to grow.

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    Extremeophiles

    Microbes that thrive in extreme environmental conditions.

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    Primary vs Secondary Metabolites

    Primary are essential for growth; secondary are for survival and defense.

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    Study Notes

    Microbiology Overview

    • Microbiology is the study of microscopic organisms, including bacteria, archaea, viruses, fungi, and protozoa.
    • Microorganisms are too small to be seen with the naked eye and require a microscope.
    • Defining microbiology is challenging as the field incorporates many sciences, and not all single-celled organisms are microscopic, and not all microscopic organisms are single-celled.

    Three-Domain System of Life

    • Carl Woese proposed a three-domain system of life: Bacteria, Archaea, and Eukarya.
    • This system classifies organisms based on their shared characteristics.

    Prokaryotes vs. Eukaryotes

    • Prokaryotes lack a nucleus and membrane-bound organelles.
    • Eukaryotes possess a nucleus and membrane-bound organelles.

    Major Groups of Microorganisms

    • Bacteria: Prokaryotic, single-celled organisms with peptidoglycan cell walls. Some are motile, and they can use organic or inorganic sources of nutrition.
    • Archaea: Prokaryotic, single-celled organisms that do not have peptidoglycan cell walls. They can use organic or inorganic sources of nutrition.
    • Eukarya: Includes protozoa, algae, fungi, and helminths (parasites). These are eukaryotic organisms and have more complex structures than prokaryotes.

    Microorganism Characteristics

    • Nutrition: Microorganisms can acquire nutrients from organic or inorganic sources.
    • Cell Walls: Bacteria and archaea usually have cell walls, but of different chemical compositions.
    • Motility: Some microorganisms can move using flagella or other structures.

    Non-Cellular Microbes

    • Viruses: DNA or RNA genome with a protein capsid, require a host cell for replication.
    • Viroids: Infectious RNA molecules.
    • Prions: Infectious proteins.

    Microbiology Contributions

    • Robert Hooke: Introduced the first compound microscope and observed "cells"
    • Antonie van Leeuwenhoek: Observed single-celled microbes, which is credited as the "father of microbiology."
    • Lazzaro Spallanzani: Demonstrated that spontaneous generation of microbes is not valid.
    • Louis Pasteur: Contributed to germ theory, and pasteurization methods.
    • Joseph Lister: Used phenol as an antiseptic for surgical instruments.
    • Robert Koch: Developed Koch's Postulates for determining the cause of infectious diseases.
    • Paul Ehrlich: Identified the first antimicrobial drug for syphilis.

    Defining Features of Microorganisms

    • Metabolic Diversity: Microbes can use various energy sources.

    • Adaptation: Certain microorganisms thrive in extreme environments.

    • Microbial Interactions: Microbes can influence each other, and other organisms.

    • Importance: Organisms play a significant role in many environmental processes, including nutrient cycling.

    Cell Structures

    • Bacterial Cell Structures (e.g., capsules, cell walls, plasmids, pili, cytoplasm, etc.)
    • Eukaryotic Cell Structures (e.g., Nucleus, mitochondria, ribosomes)

    Microbes and Disease

    • Pathogens can cause infectious diseases in humans.
    • Microbiology is relevant in understanding disease mechanisms.

    Evolutionary Relations and Endosymbiosis

    • Organisms can evolve and have relationships among themselves.
    • Endosymbiotic theory explains the evolutionary origins of eukaryotic organelles, such as mitochondria and chloroplasts, from prokaryotic cells.

    Microbial Metabolism and Nutrition

    • Microorganisms have different metabolic pathways and mechanisms to obtain nutrients from environment.
    • Microbes have an important role in carbon and nitrogen cycles.

    Microbial Growth and Reproduction

    • Microbes reproduce and grow at rates determined by the environment and factors like temperature.

    Classification

    • Microbiology has a system for classifying and naming microorganism.
    • Classification methods may be based on shared characteristics or genetics, etc.

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    Microbio Exam 1 Study Guide PDF

    Description

    Explore the fascinating world of microbiology through this quiz, which covers the basic definitions and classifications of microorganisms. Learn about the three-domain system proposed by Carl Woese, and the differences between prokaryotes and eukaryotes. Test your knowledge on major groups of microorganisms including bacteria and archaea.

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