Microbiology Laboratory Course Overview

An analytical balance is used to measure the mass of substances in the sub-milligram range.

An autoclave is used to sterilize and disinfect materials and equipment by using high-pressure steam.

A Bunsen burner is used for heating and sterilization purposes, particularly for sterilizing loop in microbiological laboratories.

<p>A centrifuge is used to separate components of a mixture by spinning the sample at high speed.</p> Signup and view all the answers

<p>A colony counter is used to estimate the density of a liquid culture by counting the number of colony-forming units (CFU) on an agar plate.</p> Signup and view all the answers

<p>A deep freezer is used for the preservation of various materials, including cultures, medical equipment, food items, and blood samples.</p> Signup and view all the answers

<p>A homogenizer is used for mixing and homogenizing different materials, such as tissue, plant, food, and soil.</p> Signup and view all the answers

<p>A hot plate is used for heating glassware and other components in a laboratory.</p> Signup and view all the answers

<p>A hot air oven is used for sterilizing medical equipment and samples using dry heat.</p> Signup and view all the answers

<p>An incubator provides controlled environmental conditions, like temperature and humidity, for the growth and maintenance of microorganisms and cultures.</p> Signup and view all the answers

<p>A laminar air flow/hood chamber creates a sterile environment for processes or instruments sensitive to microbial contamination.</p> Signup and view all the answers

<p>A magnetic stirrer is used to mix liquids, particularly in chemical or microbiological laboratories.</p> Signup and view all the answers

<p>A microscope allows magnified observation of minute particles that are invisible to the naked eye.</p> Signup and view all the answers

<p>A pH meter measures the hydrogen ion concentration in water-based solutions, determining the acidity or alkalinity.</p> Signup and view all the answers

<p>A spectrophotometer measures the intensity of light absorbed by a substance, enabling quantitative analysis.</p> Signup and view all the answers

<p>A vortex mixer is used for mixing and homogenizing samples in glass tubes or flasks.</p> Signup and view all the answers

<p>A water bath is used for heating samples at a controlled temperature, providing a consistent environment.</p> Signup and view all the answers

<p>A water distiller purifies water through distillation, creating pure distilled water.</p> Signup and view all the answers

<p>Durham tubes are used in fermentation tests to detect the production of gas.</p> Signup and view all the answers

<p>A wire loop is used to transfer microorganisms from a broth culture to a solid culture medium, like an agar plate, for culturing purposes.</p> Signup and view all the answers

<p>Bijoh bottles are commonly used in fermentation tests, especially for sugar fermentation.</p> Signup and view all the answers

<p>Proper disposal is required to prevent spreading pathogenic microorganisms, environmental contamination, and ensure safety within the lab.</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Physical Nature (A), Chemical Composition (C), Functions (D)</p> Signup and view all the answers

<p>Semisolid (A), Liquid (C), Solid (D)</p> Signup and view all the answers

<p>Solid media allows for the isolation and separation of individual bacterial species, facilitating the study of pure cultures.</p> Signup and view all the answers

<p>A colony is a visible aggregate of bacteria growing on a solid culture medium, representing a single bacterial species. A colony can be transferred to a new medium to produce that single species' pure culture.</p> Signup and view all the answers

<p>Elevation (A), Size (B), Surface Texture (C), Opacity (D), Shape (E), Margin (F), Color (G)</p> Signup and view all the answers

<p>False (B)</p> Signup and view all the answers

<p>Microbiology wastes, like cultures and contaminated materials, should be sterilized, often by autoclaving, and then incinerated or disposed of using appropriate biohazard disposal protocols.</p> Signup and view all the answers

<p>A compound light microscope typically includes an illuminator, condenser, objectives, nosepiece, stage, coarse and fine adjustment knobs, and ocular lens/eyepiece.</p> Signup and view all the answers

<p>The condenser focuses and directs the light from the illuminator onto the specimen, ensuring optimal illumination and clarity of the viewed object.</p> Signup and view all the answers

<p>The coarse adjustment knob roughly moves the objective lens up or down, bringing the specimen into focus. The fine adjustment knob fine tunes the focus, providing a clear and sharp image of the specimen.</p> Signup and view all the answers

<p>Microscopes are delicate instruments that require proper care to maintain their functionality and prevent damage. Clean objectives, proper storage, and careful handling ensure optimal performance of the microscope.</p> Signup and view all the answers