Microbiology Lab Safety Procedures Quiz

What should you use when using 40x and 100x objectives?

What is the number one problem if you have trouble focusing when switching from 10x to 40x objectives?

Which part of the microscope is used to bring the specimen into sharp focus under low power?

What is the main function of the condenser in a microscope?

What type of organisms are fungi?

What is a distinguishing characteristic of fungi as mentioned in the text?

What is the purpose of placing used microscope slides into a biohazard sharps container?

Which statement accurately describes the colonial morphology 'after' compared to 'before'?

What is the primary difference between BSL 1 and BSL 2 bacteria?

What is the correct procedure for disposing of uncontaminated broken glass in the lab?

Which bacterial shape is characterized by a cluster arrangement?

What should be done to achieve the best resolution while using a microscope?

Microscope Usage and Troubleshooting

Use immersion oil when utilizing 40x and 100x objectives to enhance resolution and clarity.
Difficulty focusing when switching from 10x to 40x objectives often stems from improper alignment or lack of sufficient light.
The coarse adjustment knob is used to bring the specimen into sharp focus under low power (10x).

Microscope Components and Functions

The condenser focuses light onto the specimen, enhancing illumination and contrast.
Proper light adjustment through the condenser is essential for optimal viewing at higher magnifications.

Microbiology: Fungi Characteristics

Fungi are eukaryotic organisms that can be unicellular or multicellular.
A distinguishing characteristic of fungi is their cell walls made of chitin, unlike plants which have cellulose.

Safety and Disposal Practices

Used microscope slides are placed in a biohazard sharps container to prevent contamination and ensure safety in the lab.
Uncontaminated broken glass should be disposed of in a designated glass disposal container, not in regular waste.

Cultural Morphology and Bacterial Classification

Colonial morphology changes can indicate different growth characteristics after incubation compared to before.
BSL 1 (Biosafety Level 1) bacteria are not known to cause disease in healthy humans, whereas BSL 2 bacteria can cause mild diseases and require specific precautions.

Bacterial Shapes and Arrangements

Staphylococcus is a bacterial genus characterized by a cluster arrangement, resembling bunches of grapes.
Achieving the best resolution while using a microscope involves optimal light adjustment, appropriate use of immersion oil, and precise focusing techniques.

Test your knowledge on microbiology lab safety procedures including hand washing, microbial types on skin, labeling of media, and disposal of biohazardous materials. Learn about the importance of following proper protocols to ensure a safe working environment in the lab.