5 Questions
Describe the steps of Gram staining a skin specimen?
The steps of Gram staining a skin specimen include: 1. Fixing the specimen with heat or alcohol. 2. Applying crystal violet stain for 1 minute. 3. Rinsing with water. 4. Applying iodine for 1 minute. 5. Rinsing with water. 6. Decolorizing with alcohol. 7. Counterstaining with safranin for 1 minute. 8. Rinsing with water and blotting dry.
What are the morphological features of common anaerobes as seen on Gram stain?
The morphological features of common anaerobes as seen on Gram stain include: 1. Small size. 2. Variable shape (pleomorphic). 3. Lack of spores. 4. Lack of oxygen requirement for growth.
Describe the handling of anaerobic specimen in the laboratories?
The handling of anaerobic specimen in the laboratories involves minimizing exposure to oxygen by using anaerobic chambers or gas packs, and ensuring prompt transport to the laboratory in anaerobic transport media or containers.
What are the steps in the preparation of a KOH mount?
The steps in the preparation of a KOH mount include: 1. Taking a small amount of specimen. 2. Placing it on a slide. 3. Adding a drop of 10-20% potassium hydroxide (KOH). 4. Covering with a coverslip. 5. Allowing the specimen to stand for 5-10 minutes before examination under a microscope.
What is the recommended method for collection of skin specimen in fungal infections?
The recommended method for collection of skin specimen in fungal infections is by scraping the edge of the lesion using a scalpel or similar instrument, and collecting the tissue or material in a sterile container for transport to the laboratory.
Test your knowledge of skin and soft tissue infections in this microbiology lab quiz. Learn about specimen collection, Gram staining, interpretation of findings, and preparation of KOH mounts for fungal infections.
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