Microbiology Lab Media
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Questions and Answers

What is the purpose of the inhibitory substance in selective media?

  • To inhibit the growth of unwanted bacteria (correct)
  • To change the color of the media
  • To promote the growth of all bacteria
  • To create an acidic environment

    • What is the characteristic of differential media?

  • They are used to promote the growth of all bacteria
  • They contain an inhibitory substance to prevent bacterial growth
  • They contain an indicator that changes color when bacteria grow (correct)
  • They are used to create an acidic environment

    • Which of the following is an example of selective media?

  • Blood agar
  • MacConkey agar
  • Nutrient agar
  • Thayer Martin agar (correct)

    • What is the purpose of the acidic environment in some media?

    <p>To inhibit the growth of unwanted bacteria</p> Signup and view all the answers

    Which of the following is an example of differential media?

    <p>Levinstein-Jensen medium</p> Signup and view all the answers

    What is the purpose of blood agar?

    <p>To encourage the growth of bacteria</p> Signup and view all the answers

    What type of bacteria can grow on blood agar?

    <p>Fastidious bacteria that may not grow on other media</p> Signup and view all the answers

    What is a characteristic of interactive media?

    <p>They contain a pH indicator that changes color when bacteria grow</p> Signup and view all the answers

    What is an example of an interactive medium?

    <p>Blood agar</p> Signup and view all the answers

    What is the main difference between blood agar and other media?

    <p>It is enriched to support the growth of fastidious bacteria</p> Signup and view all the answers

    What is the number of types of blood agar classified based on the hemolytic activity of bacteria?

    <p>Three</p> Signup and view all the answers

    What is the type of hemolysis where the bacterial colony is surrounded by a clear zone?

    <p>Beta hemolysis</p> Signup and view all the answers

    What is the characteristic of beta hemolysis?

    <p>Complete hemolysis</p> Signup and view all the answers

    Which type of hemolysis is associated with the destruction of red blood cells?

    <p>Beta hemolysis</p> Signup and view all the answers

    What is the classification of blood agar based on?

    <p>Hemolytic activity of bacteria</p> Signup and view all the answers

    What is the main purpose of Mannitol Salt Agar?

    <p>To determine if the bacteria is halophilic and can ferment mannitol</p> Signup and view all the answers

    What happens to the medium when Staphylococcus aureus ferments mannitol?

    <p>The medium turns yellow</p> Signup and view all the answers

    What characteristic of Staphylococcus aureus is detected by Mannitol Salt Agar?

    <p>Its halophilicity and ability to ferment mannitol</p> Signup and view all the answers

    What type of bacteria is Staphylococcus aureus?

    <p>Halophilic and mannitol-fermenting</p> Signup and view all the answers

    Why is Mannitol Salt Agar used in the detection of Staphylococcus aureus?

    <p>Because it can detect the bacteria's halophilicity and ability to ferment mannitol</p> Signup and view all the answers

    What is the main function of EMB agar?

    <p>To inhibit the growth of most Gram-negative bacteria</p> Signup and view all the answers

    What is the primary role of methylene blue in EMB agar?

    <p>To indicate the pH level of the medium</p> Signup and view all the answers

    What is the characteristic of EMB agar that makes it differential?

    <p>It indicates the acidity of the medium through a color change</p> Signup and view all the answers

    What is the result of E. coli growth on EMB agar?

    <p>A green, metallic sheen</p> Signup and view all the answers

    What type of bacteria is EMB agar primarily used to isolate?

    <p>Anaerobic bacteria</p> Signup and view all the answers

    Study Notes

    Microbiology Media

    • Differential media contain an indicator that changes color when bacteria grow, e.g. Blood agar, MacConkey agar.
    • Blood agar is an enriched, bacterial growth medium that encourages bacterial growth, especially for Streptococcus that may not grow normally in other media.

    Types of Blood Agar

    • Can be classified into three types based on hemolysis activity:
    • Alpha (α) hemolysis (partial hemolysis)
    • Beta (β) hemolysis (complete hemolysis), e.g. Staphylococcus aureus, which ferments mannitol and turns the medium yellow.
    • Gamma (γ) hemolysis (no hemolysis)

    Mannitol Salt Agar (MSA)

    • Used to determine if bacteria are halophilic (salt-loving) and can ferment mannitol.
    • Staphylococcus aureus ferments mannitol and turns the medium yellow.

    Eosin Methylene Blue (EMB) Agar

    • A selective and differential medium used to isolate coliform bacteria.
    • Inhibits the growth of most Gram-positive bacteria.
    • The methylene blue indicator shows the degree of acidity.
    • If bacteria produce strong acid, such as E. coli, it leads to a greenish metallic color.

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    Description

    This quiz covers types of media used in microbiology lab, including selective and differential media, and their components.

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