Podcast
Questions and Answers
What is the primary relationship between the normal microbiota and the host?
What is the primary relationship between the normal microbiota and the host?
Which term describes the disruption of normal microbiota?
Which term describes the disruption of normal microbiota?
Which of the following is a consequence of dysbiosis?
Which of the following is a consequence of dysbiosis?
What does tropism refer to in the context of pathogens?
What does tropism refer to in the context of pathogens?
Signup and view all the answers
How can antibiotics affect the normal microbiota?
How can antibiotics affect the normal microbiota?
Signup and view all the answers
Why might a commensal organism become pathogenic in another host?
Why might a commensal organism become pathogenic in another host?
Signup and view all the answers
Which of the following statements is true regarding host–microbe interactions?
Which of the following statements is true regarding host–microbe interactions?
Signup and view all the answers
What is a characteristic of pathogens?
What is a characteristic of pathogens?
Signup and view all the answers
What is the term that describes the preference of a pathogen for a specific host or tissue?
What is the term that describes the preference of a pathogen for a specific host or tissue?
Signup and view all the answers
Which of the following factors can influence whether a pathogen will cause disease in a host?
Which of the following factors can influence whether a pathogen will cause disease in a host?
Signup and view all the answers
What is the difference between ID50 and LD50?
What is the difference between ID50 and LD50?
Signup and view all the answers
What is one way that pathogens can evade host immune defenses?
What is one way that pathogens can evade host immune defenses?
Signup and view all the answers
Which of the following is considered an opportunistic pathogen?
Which of the following is considered an opportunistic pathogen?
Signup and view all the answers
Which of the following is NOT a method by which pathogens can hide from the immune system?
Which of the following is NOT a method by which pathogens can hide from the immune system?
Signup and view all the answers
What role does the immune system play in relation to normal microbiota?
What role does the immune system play in relation to normal microbiota?
Signup and view all the answers
What is a characteristic of intracellular pathogens?
What is a characteristic of intracellular pathogens?
Signup and view all the answers
What can contribute to the development of septic shock in a host?
What can contribute to the development of septic shock in a host?
Signup and view all the answers
Which of the following statements is true regarding the relationship between pathogens and hosts?
Which of the following statements is true regarding the relationship between pathogens and hosts?
Signup and view all the answers
Which of the following pathogens is known for causing latent infections?
Which of the following pathogens is known for causing latent infections?
Signup and view all the answers
What is a potential consequence of a pathogen remaining in a latent state?
What is a potential consequence of a pathogen remaining in a latent state?
Signup and view all the answers
How is virulence best viewed?
How is virulence best viewed?
Signup and view all the answers
Which immune defense strategy involves the pathogen damaging phagocytic cells?
Which immune defense strategy involves the pathogen damaging phagocytic cells?
Signup and view all the answers
How do pathogens utilize antigen masking as a strategy?
How do pathogens utilize antigen masking as a strategy?
Signup and view all the answers
Which of the following is an example of intracellular bacteria?
Which of the following is an example of intracellular bacteria?
Signup and view all the answers
Which age group was more significantly affected by the influenza pandemic of 1918?
Which age group was more significantly affected by the influenza pandemic of 1918?
Signup and view all the answers
What is the R0 (R-naught) value used to measure?
What is the R0 (R-naught) value used to measure?
Signup and view all the answers
What does effective reproduction number (Re) represent during an epidemic?
What does effective reproduction number (Re) represent during an epidemic?
Signup and view all the answers
How do pathogens often become attenuated when grown in cell culture?
How do pathogens often become attenuated when grown in cell culture?
Signup and view all the answers
In typical flu outbreaks, which population tends to suffer higher mortality rates?
In typical flu outbreaks, which population tends to suffer higher mortality rates?
Signup and view all the answers
What is a significant factor that can influence a pathogen's virulence?
What is a significant factor that can influence a pathogen's virulence?
Signup and view all the answers
Which of the following is true about asymptomatic infections in children for SARS-CoV-2?
Which of the following is true about asymptomatic infections in children for SARS-CoV-2?
Signup and view all the answers
What is one characteristic of an attenuated pathogen?
What is one characteristic of an attenuated pathogen?
Signup and view all the answers
What is the primary function of antigenic masking in pathogens?
What is the primary function of antigenic masking in pathogens?
Signup and view all the answers
How does antigenic mimicry assist a pathogen in evading the host immune system?
How does antigenic mimicry assist a pathogen in evading the host immune system?
Signup and view all the answers
What is one of the mechanisms through which pathogens can interfere with phagocytosis?
What is one of the mechanisms through which pathogens can interfere with phagocytosis?
Signup and view all the answers
What is antigenic variation primarily responsible for in pathogens?
What is antigenic variation primarily responsible for in pathogens?
Signup and view all the answers
Which of the following is NOT a method by which pathogens can limit the immune system's actions?
Which of the following is NOT a method by which pathogens can limit the immune system's actions?
Signup and view all the answers
What causes antigenic variation in pathogens?
What causes antigenic variation in pathogens?
Signup and view all the answers
What role does a capsule play in the context of immune evasion?
What role does a capsule play in the context of immune evasion?
Signup and view all the answers
What is a potential consequence of a pathogen thriving inside the phagolysosome?
What is a potential consequence of a pathogen thriving inside the phagolysosome?
Signup and view all the answers
What is the primary purpose of standard precautions in healthcare settings?
What is the primary purpose of standard precautions in healthcare settings?
Signup and view all the answers
What precautions should be taken before and after patient contact?
What precautions should be taken before and after patient contact?
Signup and view all the answers
Which of the following is part of contact precautions?
Which of the following is part of contact precautions?
Signup and view all the answers
Which infectious agents would typically require droplet precautions?
Which infectious agents would typically require droplet precautions?
Signup and view all the answers
What is the role of signage in transmission precautions?
What is the role of signage in transmission precautions?
Signup and view all the answers
What must be done during patient transport when droplet precautions are in place?
What must be done during patient transport when droplet precautions are in place?
Signup and view all the answers
Which equipment is typically dedicated for single-patient use under contact precautions?
Which equipment is typically dedicated for single-patient use under contact precautions?
Signup and view all the answers
Which type of precautions would be necessary for a patient with tuberculosis?
Which type of precautions would be necessary for a patient with tuberculosis?
Signup and view all the answers
Flashcards
Host-microbe interactions
Host-microbe interactions
Dynamic interactions between a host (e.g., human) and microbes, that can be beneficial, harmful, or neutral.
Normal microbiota
Normal microbiota
Microbes that reside in a host without causing disease.
Mutualistic relationship
Mutualistic relationship
Relationship in which both the host and the microbe benefit.
Pathogens
Pathogens
Signup and view all the flashcards
Dysbiosis
Dysbiosis
Signup and view all the flashcards
Clostridioides difficile
Clostridioides difficile
Signup and view all the flashcards
Tropism
Tropism
Signup and view all the flashcards
Commensal organism
Commensal organism
Signup and view all the flashcards
Opportunistic Pathogens
Opportunistic Pathogens
Signup and view all the flashcards
Tropism (pathogen)
Tropism (pathogen)
Signup and view all the flashcards
Pathogenicity
Pathogenicity
Signup and view all the flashcards
Virulence
Virulence
Signup and view all the flashcards
Virulence Factors
Virulence Factors
Signup and view all the flashcards
ID50
ID50
Signup and view all the flashcards
LD50
LD50
Signup and view all the flashcards
Transmission and virulence
Transmission and virulence
Signup and view all the flashcards
R0 (R-naught)
R0 (R-naught)
Signup and view all the flashcards
Re, effective reproduction number
Re, effective reproduction number
Signup and view all the flashcards
Host Factors
Host Factors
Signup and view all the flashcards
Attenuated pathogen
Attenuated pathogen
Signup and view all the flashcards
Influences on virulence
Influences on virulence
Signup and view all the flashcards
Evolving virulence
Evolving virulence
Signup and view all the flashcards
Immune System Damage
Immune System Damage
Signup and view all the flashcards
Tuberculosis Tissue Damage
Tuberculosis Tissue Damage
Signup and view all the flashcards
Viral Infections and Immune Response
Viral Infections and Immune Response
Signup and view all the flashcards
Pathogen's Goal: Replicate
Pathogen's Goal: Replicate
Signup and view all the flashcards
Hiding from the Immune System
Hiding from the Immune System
Signup and view all the flashcards
Intracellular Pathogens
Intracellular Pathogens
Signup and view all the flashcards
Latency
Latency
Signup and view all the flashcards
Herpes Viruses and Latency
Herpes Viruses and Latency
Signup and view all the flashcards
Antigenic Masking
Antigenic Masking
Signup and view all the flashcards
Antigenic Mimicry
Antigenic Mimicry
Signup and view all the flashcards
Antigenic Variation
Antigenic Variation
Signup and view all the flashcards
Interference with Phagocytosis
Interference with Phagocytosis
Signup and view all the flashcards
How can pathogens interfere with phagocytosis?
How can pathogens interfere with phagocytosis?
Signup and view all the flashcards
Why can pathogens thrive in the phagolysosome?
Why can pathogens thrive in the phagolysosome?
Signup and view all the flashcards
How do pathogens undermine the host immune response?
How do pathogens undermine the host immune response?
Signup and view all the flashcards
Standard Precautions
Standard Precautions
Signup and view all the flashcards
What are the components of Standard Precautions?
What are the components of Standard Precautions?
Signup and view all the flashcards
Transmission Precautions
Transmission Precautions
Signup and view all the flashcards
Contact Precautions
Contact Precautions
Signup and view all the flashcards
Droplet Precautions
Droplet Precautions
Signup and view all the flashcards
Airborne Precautions
Airborne Precautions
Signup and view all the flashcards
Why are Transmission Precautions important?
Why are Transmission Precautions important?
Signup and view all the flashcards
How are Transmission Precautions chosen?
How are Transmission Precautions chosen?
Signup and view all the flashcards
Study Notes
Microbiology: Basic and Clinical Principles
- The text is a chapter 10 from a microbiology textbook titled Microbiology: Basic and Clinical Principles (2nd Edition).
- The chapter covers host-microbe interactions and pathogenesis.
- A clinical case study, The Case of the Deadly Mistake, is presented, encouraging students to explore the topic further in the online Mastering Microbiology study area.
- The study area materials will use host-microbe interactions to explain a medical mystery.
Basics of Host-Microbe Interactions
- Understanding host-microbe interactions is crucial to understanding health and disease.
- Shifts in the normal microbiota, including changes in levels or location, can contribute to disease.
- A commensal organism in one host can be pathogenic in another host due to differences in host factors.
- Tropism, the pathogen's preference for a specific host or tissue, influences pathogen emergence.
Host-Microbe Interactions Are Not Always Harmful
- Host-microbe interactions involve give-and-take dynamics.
- Normal microbiota colonize various body systems (skin, digestive, genital, urinary, respiratory).
- Certain microbiota benefit the host by competing with pathogens, producing vitamins, and promoting immune system development.
- These relationships between microbes and the host are often mutualistic.
- Disrupting the normal microbiota balance compromises patient health.
- Pathogens are disease-causing microbes that adapt to interact with specific host tissues and are dangerous to the host.
- Dysbiosis (microbiota disruption) occurs due to antibiotics killing off normal microbiota, allowing usually minor, resident pathogens to flourish (e.g., Clostridioides difficile).
Tropism is the Preference of a Pathogen for a Specific Host
- All microbes need specific host features for infection.
- Tropism is the pathogen's preference for a specific host (and tissue within the host).
- Most microbes show tropism, but this can change over time, influencing pathogen emergence.
- Even if a host is a preferred target within a pathogen's tropism, the pathogen's ability to cause disease in that host depends on host factors and characteristics.
Introduction to Virulence
- Pathogenicity is a microbe's capacity to cause disease.
- Virulence describes the severity of disease caused by a pathogen.
- Virulence factors are mechanisms pathogens use to overcome host defenses, adhere to cells, and invade tissues.
- Ro and Re (basic and effective reproduction numbers) are factors significant for epidemic/pandemic management.
- Virulence is best understood as an evolving property in response to host and environmental pressures.
- Attenuated pathogens are weakened versions often used in vaccines.
Host-Microbe Interactions Influence Virulence
- Pathogenicity describes the microbe's ability to cause disease.
- Virulence describes the extent of disease a pathogen causes.
- Virulence factors are mechanisms pathogens use to overcome host defenses, adhere to cells, and invade tissues. Mechanisms include toxins, adhesion factors, invasion factors, and evasion of host immune defenses.
Host Factors and Virulence
- Virulence is linked to host factors like immune response, immune fitness, and microbiota balance.
- An example is a more severe response in young, healthy adults experiencing the 1918 influenza pandemic. A similar effect is seen with SARS-CoV-2 infections having a less severe or even asymptomatic impact on children.
Transmission and Virulence
- Virulence factors are linked to transmission.
- A pathogen's transmission capability (or contagiousness) is measured by basic reproduction number (Ro)- the expected number of infections caused by one infected individual in a wholly susceptible population.
- A more appropriate measure during epidemics and pandemics is the effective reproduction number (Re). The Ro and Re values depend on host-pathogen interactions.
A Pathogen's Environment Influences Virulence
- Virulence is an evolving property of pathogens, changing in response to host and environmental factors.
- Pathogens evolve to create new virulence factors in response to host defenses and selective pressures.
- Attenuated pathogens, useful in vaccines, develop decreased virulence factors after growth in cell culture.
The Dosage of Pathogen and Toxin Exposure
- For a pathogen to cause disease, it must first infect the host.
- The infectious dose-50 (ID50) is the amount of pathogen needed to infect 50% of exposed hosts. A lower ID50 indicates a more infectious pathogen.
- The lethal dose-50 (LD50) is the amount of toxin needed to kill 50% of affected hosts. A low LD50 indicates the pathogen produces a highly toxic toxin.
Toxins are Major Virulence Factors
- Toxins are molecules generating effects like tissue damage, and suppressed immune responses.
- Toxigenic microbes produce toxins.
- Toxemia is toxins in the bloodstream.
- Two main classes of toxins: endotoxins and exotoxins.
Endotoxins
- A component (Lipid A) of lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria.
- Released when Gram-negative bacteria die.
- High quantities of endotoxins can induce a strong immune response and lead to complications such as fever, chills, body aches, hypotension, and vomiting (symptoms of septic shock).
- Endotoxemia is endotoxin in the blood stream, a potential consequence of infection and/or treatment use.
Exotoxins
- Soluble proteins that affect a wide range of cells.
- Made by both Gram-positive and Gram-negative bacteria.
- Classified based on organism or type of cell affected (e.g., neurotoxins, enterotoxins, hepatoxins, nephrotoxins).
- Three main groups according to their mechanisms of action (Membrane-acting exotoxins, Membrane-damaging exotoxins, Intracellular AB toxins)
Five Steps to Infection
- Pathogens successfully infect a host following these five steps: entering the host, adherence to tissues, invasion and obtaining nutrients, replication while evading the immune system, and transmission to a new host.
First, a Pathogen Must Enter a Host
- Portals of entry are the sites pathogens use to enter the host.
- Mucous membranes frequently serve as the primary portal of entry.
Skin, Ocular, Otic, and Parenteral Entry
- The largest body system, the integumentary system (skin, hair, nails, glands) blocks most microbes.
- Some pathogens have developed virulence factors to overcome this defense, including getting in through abrasions, bites, injections, and surgical incisions.
Respiratory Tract Entry
- The respiratory tract is usually a common portal of entry because coughing and sneezing spread infectious agents in droplets.
- Infectious agents can also be stirred into the air from dust or soil.
Gastrointestinal (GI) Tract Entry
- GI tract infection pathogens frequently use fecal-oral transmission.
Urogenital Tract and Transplacental Entry
- Sexually transmitted pathogens enter via the vaginal/cervical or urethral mucosa.
- Some pathogens utilize transplacental entry (e.g., HIV).
Second, a Pathogen Must Adhere to Host Tissues
- Adhesion is often nonspecific.
- Specific mechanisms may be used (via receptors) to adhere to the host cell.
Adhesion Factors
- Adhesins are virulence factors assisting in pathogen adhesion to host tissues.
- These can be cell wall components, capsules, or plasma membrane molecules.
Biofilms and Quorum Sensing
- Bacteria develop biofilms on various surfaces.
- Healthcare-associated biofilms frequently form on implanted devices or medical prosthetics.
Third, a Pathogen Must Invade Tissues and Obtain Nutrients
- Pathogens have several options after adhering:
- Stay on the host cell surface,
- Pass through cells to deeper tissues,
- Enter cells as intracellular pathogens
- Invasins help pathogens invade host tissues, including proteases, collagenases, and lipases.
Invasins and Motility
- Invasins allow pathogens to invade host tissues.
- Motility helps a pathogen spread to another location within a host or to a new host.
Tools to Obtain Nutrients: Siderophores and Extracellular Enzymes
- Cellular pathogens require iron for survival.
- Transferrin shuttles iron to tissues (and blood.)
- Many bacteria produce siderophores that capture free iron from the host.
- Extracellular enzymes from bacteria and fungi break down local nutrients to aid in getting nutrients. Examples include lipases (lipids) and proteases (proteins).
Cytopathic Effects in the Host
- Pathogens damage host cells, generating cytocidal (killing cells) and noncytocidal (damaging cells) effects.
- Immune systems can also cause damage in the body as by-products of combating infections.
Fourth, a Pathogen Must Evade Host Immune Defenses So It Can Replicate
- Hiding and undermining defenses are critical to successful pathogen replication. Methods include intracellular lifestyle, latency, antibody masking/mimicry, antigenic variation, and interfering with phagocytosis, host immune responses.
Hiding from Host Immune Defenses
- Intracellular pathogens include viruses, some protozoans, and certain bacteria.
- Latency is when pathogens lie dormant within a host, avoiding detection.
- Antigenic masking/mimicry is when pathogens conceal their antigens to mask identification by the host's immune system.
- Antigenic variation occurs when pathogens vary surface molecules to evade a rapid immune response.
Undermining the Host Immune Response
- Pathogens can interfere with phagocytosis, damaging phagocytic cells by releasing toxins, altering the host's immune response, or neutralising enzymes.
Fifth, a Pathogen Must Be Transmitted to a New Host to Repeat the Cycle
- The last step in establishing an infection is transmission to a new host.
- Symptoms such as itchiness, sneezing, coughing, and diarrhea facilitates transmission to new hosts.
Exiting the Host
- Portals of exit are the routes pathogens use to leave their host, aiding further transmission.
- Common examples include feces, urine, bodily fluids, wounds.
Maintaining a Reservoir
- A pathogen's reservoir is the environment or organism from which pathogens maintain their lifecycle.
- Environmental niches such as soil, water can serve as reservoirs.
- Microorganismal reservoirs include humans or animals.
Safety and Health Care
- Biosafety levels (BSL) help identify critical criteria for assigning pathogens to a BSL level.
- There are four biosafety level categories: BSL-1, BSL-2, BSL-3, BSL-4.
- Each BSL has associated specific personal protective equipment (PPE).
Infection Control Practices
- Most healthcare facilities have infection control teams.
- Standard precautions and transmission precautions are essential for limiting infection risk for healthcare workers and patients.
- Standard precautions treat all patients as potential sources of infection.
- Transmission precautions are used when a specific infectious agent is suspected, including contact, droplet, or airborne precautions.
Think Clinically: Be SMART About Cases
- A case study of a patient with a hand wound that developed into a serious infection is presented.
Studying That Suits You
Use AI to generate personalized quizzes and flashcards to suit your learning preferences.
Related Documents
Description
Test your knowledge on the relationships between normal microbiota and hosts, the effects of dysbiosis, and the characteristics of pathogens. Explore how antibiotics can impact the microbiota and the immune system's role against pathogens. This quiz covers various aspects of host-microbe interactions.