Microbiology Host-Pathogen Interactions Quiz
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Questions and Answers

What is the primary relationship between the normal microbiota and the host?

  • They compete for resources.
  • They remain completely independent.
  • They only exist to cause harm.
  • They mutually benefit each other. (correct)
  • Which term describes the disruption of normal microbiota?

  • Homeostasis
  • Dysbiosis (correct)
  • Stabilization
  • Symbiosis
  • Which of the following is a consequence of dysbiosis?

  • Bacterial colonization of new areas
  • Increased competition with normal microbiota
  • Improved immune response
  • Flourishing of harmful pathogens (correct)
  • What does tropism refer to in the context of pathogens?

    <p>The tendency of a pathogen to infect certain host tissues (A)</p> Signup and view all the answers

    How can antibiotics affect the normal microbiota?

    <p>By killing off normal microbiota and allowing pathogens to flourish (B)</p> Signup and view all the answers

    Why might a commensal organism become pathogenic in another host?

    <p>The environment of the new host is more suitable. (B)</p> Signup and view all the answers

    Which of the following statements is true regarding host–microbe interactions?

    <p>Normal microbiota can help prevent infections. (B)</p> Signup and view all the answers

    What is a characteristic of pathogens?

    <p>They adapt to interact with specific host tissues. (D)</p> Signup and view all the answers

    What is the term that describes the preference of a pathogen for a specific host or tissue?

    <p>Tropism (D)</p> Signup and view all the answers

    Which of the following factors can influence whether a pathogen will cause disease in a host?

    <p>Host age (B)</p> Signup and view all the answers

    What is the difference between ID50 and LD50?

    <p>One measures infection, while the other measures lethality. (D)</p> Signup and view all the answers

    What is one way that pathogens can evade host immune defenses?

    <p>Suppress immune function (C)</p> Signup and view all the answers

    Which of the following is considered an opportunistic pathogen?

    <p>Escherichia coli in the appendix (D)</p> Signup and view all the answers

    Which of the following is NOT a method by which pathogens can hide from the immune system?

    <p>Inducing fever (A)</p> Signup and view all the answers

    What role does the immune system play in relation to normal microbiota?

    <p>It maintains a balanced communication with them. (B)</p> Signup and view all the answers

    What is a characteristic of intracellular pathogens?

    <p>They spend a majority of their time inside host cells. (C)</p> Signup and view all the answers

    What can contribute to the development of septic shock in a host?

    <p>Endotoxins (C)</p> Signup and view all the answers

    Which of the following statements is true regarding the relationship between pathogens and hosts?

    <p>Certain host factors can prevent pathogen-induced disease. (D)</p> Signup and view all the answers

    Which of the following pathogens is known for causing latent infections?

    <p>Herpes viruses (D)</p> Signup and view all the answers

    What is a potential consequence of a pathogen remaining in a latent state?

    <p>Protection from drug therapies (D)</p> Signup and view all the answers

    How is virulence best viewed?

    <p>As an evolving property. (A)</p> Signup and view all the answers

    Which immune defense strategy involves the pathogen damaging phagocytic cells?

    <p>Secretion of toxins (B)</p> Signup and view all the answers

    How do pathogens utilize antigen masking as a strategy?

    <p>By hiding their surface antigens from detection (A)</p> Signup and view all the answers

    Which of the following is an example of intracellular bacteria?

    <p>Mycobacterium tuberculosis (A)</p> Signup and view all the answers

    Which age group was more significantly affected by the influenza pandemic of 1918?

    <p>Young adults (D)</p> Signup and view all the answers

    What is the R0 (R-naught) value used to measure?

    <p>A pathogen's transmissibility (D)</p> Signup and view all the answers

    What does effective reproduction number (Re) represent during an epidemic?

    <p>The changes in transmission dynamics over the course of an outbreak (B)</p> Signup and view all the answers

    How do pathogens often become attenuated when grown in cell culture?

    <p>They lose virulence factors needed to cause disease (C)</p> Signup and view all the answers

    In typical flu outbreaks, which population tends to suffer higher mortality rates?

    <p>Elderly patients (D)</p> Signup and view all the answers

    What is a significant factor that can influence a pathogen's virulence?

    <p>Environmental and host factors (B)</p> Signup and view all the answers

    Which of the following is true about asymptomatic infections in children for SARS-CoV-2?

    <p>Children are often asymptomatic (C)</p> Signup and view all the answers

    What is one characteristic of an attenuated pathogen?

    <p>It is infectious but does not cause disease in immunocompetent hosts (D)</p> Signup and view all the answers

    What is the primary function of antigenic masking in pathogens?

    <p>To conceal antigenic features using host molecules (B)</p> Signup and view all the answers

    How does antigenic mimicry assist a pathogen in evading the host immune system?

    <p>By producing surface proteins that resemble host molecules (A)</p> Signup and view all the answers

    What is one of the mechanisms through which pathogens can interfere with phagocytosis?

    <p>Bursting free from the phagosome (C)</p> Signup and view all the answers

    What is antigenic variation primarily responsible for in pathogens?

    <p>Preventing a rapid immune response (D)</p> Signup and view all the answers

    Which of the following is NOT a method by which pathogens can limit the immune system's actions?

    <p>Enhancing immune cell signals (B)</p> Signup and view all the answers

    What causes antigenic variation in pathogens?

    <p>Mutations in the pathogen's genome (D)</p> Signup and view all the answers

    What role does a capsule play in the context of immune evasion?

    <p>It makes phagocytosis more difficult (D)</p> Signup and view all the answers

    What is a potential consequence of a pathogen thriving inside the phagolysosome?

    <p>Ability to overcome harsh environmental conditions (C)</p> Signup and view all the answers

    What is the primary purpose of standard precautions in healthcare settings?

    <p>To limit transmission of bloodborne pathogens (D)</p> Signup and view all the answers

    What precautions should be taken before and after patient contact?

    <p>Hand washing is recommended (B)</p> Signup and view all the answers

    Which of the following is part of contact precautions?

    <p>Barrier gowns and gloves are worn (C)</p> Signup and view all the answers

    Which infectious agents would typically require droplet precautions?

    <p>Rubella and influenza (D)</p> Signup and view all the answers

    What is the role of signage in transmission precautions?

    <p>To inform about necessary precautions (C)</p> Signup and view all the answers

    What must be done during patient transport when droplet precautions are in place?

    <p>The patient should wear a mask (C)</p> Signup and view all the answers

    Which equipment is typically dedicated for single-patient use under contact precautions?

    <p>Noncritical equipment (B)</p> Signup and view all the answers

    Which type of precautions would be necessary for a patient with tuberculosis?

    <p>Airborne precautions (C)</p> Signup and view all the answers

    Flashcards

    Host-microbe interactions

    Dynamic interactions between a host (e.g., human) and microbes, that can be beneficial, harmful, or neutral.

    Normal microbiota

    Microbes that reside in a host without causing disease.

    Mutualistic relationship

    Relationship in which both the host and the microbe benefit.

    Pathogens

    Microbes that cause disease.

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    Dysbiosis

    Disturbance or imbalance in the normal microbiota.

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    Clostridioides difficile

    A microbe that can cause disease if the normal microbiota is disrupted by treatment (e.g., antibiotics).

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    Tropism

    The preference of a pathogen for certain host tissues or organs.

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    Commensal organism

    An organism that lives in or on another organism without harming or benefiting it.

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    Opportunistic Pathogens

    Microbes that cause disease only under specific circumstances, such as weakened immune systems or introduction to an unusual location in the body.

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    Tropism (pathogen)

    A pathogen's preference for a specific host or tissue within that host.

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    Pathogenicity

    The ability of a microbe to cause disease.

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    Virulence

    The degree or intensity of pathogenicity.

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    Virulence Factors

    Characteristics of a pathogen that help it cause disease.

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    ID50

    The infectious dose that will make 50% of the exposed population sick.

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    LD50

    The lethal dose that will kill 50% of the exposed population.

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    Transmission and virulence

    The connection between how a pathogen spreads and how harmful it is.

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    R0 (R-naught)

    A pathogen's basic reproduction number; how many new cases one infected person creates, on average

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    Re, effective reproduction number

    Measures a pathogen's contagiousness during an epidemic/pandemic; it changes as the situation evolves.

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    Host Factors

    Characteristics of a host that can influence a pathogen's virulence.

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    Attenuated pathogen

    A pathogen weakened but still infectious, often losing the traits that cause disease.

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    Influences on virulence

    Virulence changes because some pathogens evolve new hurtful traits that can adapt to their surroundings and the hosts they infect.

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    Evolving virulence

    Virulence is a characteristic that changes as pathogens react to and interact with the environment and the hosts they infect.

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    Immune System Damage

    The immune system can damage the body as a byproduct of fighting infections. This is due to the immune system attacking infected cells.

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    Tuberculosis Tissue Damage

    The tissue damage in tuberculosis is caused by the immune system attacking cells infected with the bacteria causing the disease.

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    Viral Infections and Immune Response

    Most viral infections are fought off by the immune system killing virus-infected cells.

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    Pathogen's Goal: Replicate

    A pathogen must evade the host's immune system to safely replicate and spread.

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    Hiding from the Immune System

    Pathogens can use various strategies to hide from the immune system.

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    Intracellular Pathogens

    These pathogens spend most of their life cycle inside host cells, making them difficult for the immune system to target.

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    Latency

    The ability of a pathogen to quietly exist inside a host without causing immediate symptoms. This can lead to intermittent outbreaks or persistent infections.

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    Herpes Viruses and Latency

    Herpes viruses are an example of pathogens that use latency. They remain dormant but can cause flare-ups or reactivations.

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    Antigenic Masking

    A pathogen hides its antigens by coating itself with host molecules, evading the immune system.

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    Antigenic Mimicry

    A pathogen deceives the immune system by mimicking host molecules, such as carbohydrates, using its own capsule.

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    Antigenic Variation

    Pathogens periodically change their surface molecules to evade a rapid immune response.

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    Interference with Phagocytosis

    Pathogens limit the immune system's ability to engulf and destroy them by various mechanisms, such as forming capsules, bursting free of phagosomes, or neutralizing phagocytic enzymes.

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    How can pathogens interfere with phagocytosis?

    Pathogens can avoid phagocytosis by forming capsules, bursting free of phagosomes, blocking fusion of the phagosome with the lysosome, neutralizing phagocytic enzymes, or damaging phagocytic cells with toxins.

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    Why can pathogens thrive in the phagolysosome?

    Some pathogens have evolved to survive within the harsh environment of the phagolysosome, the destructive compartment inside phagocytes.

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    How do pathogens undermine the host immune response?

    Pathogens can limit the immune response by interfering with phagocytosis (the process of engulfing and destroying pathogens) or by suppressing the immune response altogether.

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    Standard Precautions

    A set of infection control practices used to prevent the spread of infections in healthcare settings. They apply to all patients, regardless of their suspected infection status.

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    What are the components of Standard Precautions?

    Standard precautions include hand hygiene, wearing gloves between tasks or procedures, using barrier clothing and face shields, proper management of biohazard waste, and disinfection of surfaces.

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    Transmission Precautions

    Additional infection control measures implemented when specific infectious agents are suspected or confirmed. These precautions are tailored to the mode of transmission of the pathogen.

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    Contact Precautions

    Precautions used to prevent the spread of infections through direct contact with an infected patient or contaminated objects. They include wearing gown and gloves, disinfecting surfaces, and limiting patient transport.

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    Droplet Precautions

    Precautions used to prevent the spread of infections through droplets produced by coughing, sneezing, or talking. They include wearing a mask, limiting patient transport, and having the patient wear a mask during transport.

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    Airborne Precautions

    Precautions used to prevent the spread of infections through small particles in the air that remain suspended for long periods. They include isolating the patient in a specialized negative pressure room and wearing a respirator mask.

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    Why are Transmission Precautions important?

    Transmission precautions help to prevent the spread of infections within healthcare settings, protecting both healthcare workers and patients. They are crucial for controlling outbreaks and ensuring patient safety.

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    How are Transmission Precautions chosen?

    The choice of transmission precautions depends on the suspected or confirmed infectious agent and its mode of transmission. Healthcare providers use their knowledge of infectious diseases to determine the appropriate precautions.

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    Study Notes

    Microbiology: Basic and Clinical Principles

    • The text is a chapter 10 from a microbiology textbook titled Microbiology: Basic and Clinical Principles (2nd Edition).
    • The chapter covers host-microbe interactions and pathogenesis.
    •  A clinical case study, The Case of the Deadly Mistake, is presented, encouraging students to explore the topic further in the online Mastering Microbiology study area.
    • The study area materials will use host-microbe interactions to explain a medical mystery.

    Basics of Host-Microbe Interactions

    • Understanding host-microbe interactions is crucial to understanding health and disease.
    • Shifts in the normal microbiota, including changes in levels or location, can contribute to disease.
    • A commensal organism in one host can be pathogenic in another host due to differences in host factors.
    • Tropism, the pathogen's preference for a specific host or tissue, influences pathogen emergence.

    Host-Microbe Interactions Are Not Always Harmful

    • Host-microbe interactions involve give-and-take dynamics.
    • Normal microbiota colonize various body systems (skin, digestive, genital, urinary, respiratory).
    • Certain microbiota benefit the host by competing with pathogens, producing vitamins, and promoting immune system development.
    • These relationships between microbes and the host are often mutualistic.
    • Disrupting the normal microbiota balance compromises patient health.
    • Pathogens are disease-causing microbes that adapt to interact with specific host tissues and are dangerous to the host.
    • Dysbiosis (microbiota disruption) occurs due to antibiotics killing off normal microbiota, allowing usually minor, resident pathogens to flourish (e.g., Clostridioides difficile).

    Tropism is the Preference of a Pathogen for a Specific Host

    • All microbes need specific host features for infection.
    • Tropism is the pathogen's preference for a specific host (and tissue within the host).
    • Most microbes show tropism, but this can change over time, influencing pathogen emergence.
    • Even if a host is a preferred target within a pathogen's tropism, the pathogen's ability to cause disease in that host depends on host factors and characteristics.

    Introduction to Virulence

    • Pathogenicity is a microbe's capacity to cause disease.
    • Virulence describes the severity of disease caused by a pathogen.
    • Virulence factors are mechanisms pathogens use to overcome host defenses, adhere to cells, and invade tissues.
    • Ro and Re (basic and effective reproduction numbers) are factors significant for epidemic/pandemic management.
    • Virulence is best understood as an evolving property in response to host and environmental pressures.
    • Attenuated pathogens are weakened versions often used in vaccines.

    Host-Microbe Interactions Influence Virulence

    • Pathogenicity describes the microbe's ability to cause disease.
    • Virulence describes the extent of disease a pathogen causes.
    • Virulence factors are mechanisms pathogens use to overcome host defenses, adhere to cells, and invade tissues. Mechanisms include toxins, adhesion factors, invasion factors, and evasion of host immune defenses.

    Host Factors and Virulence

    • Virulence is linked to host factors like immune response, immune fitness, and microbiota balance.
    • An example is a more severe response in young, healthy adults experiencing the 1918 influenza pandemic. A similar effect is seen with SARS-CoV-2 infections having a less severe or even asymptomatic impact on children.

    Transmission and Virulence

    • Virulence factors are linked to transmission.
    • A pathogen's transmission capability (or contagiousness) is measured by basic reproduction number (Ro)- the expected number of infections caused by one infected individual in a wholly susceptible population.
    • A more appropriate measure during epidemics and pandemics is the effective reproduction number (Re). The Ro and Re values depend on host-pathogen interactions.

    A Pathogen's Environment Influences Virulence

    • Virulence is an evolving property of pathogens, changing in response to host and environmental factors.
    • Pathogens evolve to create new virulence factors in response to host defenses and selective pressures.
    • Attenuated pathogens, useful in vaccines, develop decreased virulence factors after growth in cell culture.

    The Dosage of Pathogen and Toxin Exposure

    • For a pathogen to cause disease, it must first infect the host.
    • The infectious dose-50 (ID50) is the amount of pathogen needed to infect 50% of exposed hosts. A lower ID50 indicates a more infectious pathogen.
    • The lethal dose-50 (LD50) is the amount of toxin needed to kill 50% of affected hosts. A low LD50 indicates the pathogen produces a highly toxic toxin.

    Toxins are Major Virulence Factors

    • Toxins are molecules generating effects like tissue damage, and suppressed immune responses.
    • Toxigenic microbes produce toxins.
    • Toxemia is toxins in the bloodstream.
    • Two main classes of toxins: endotoxins and exotoxins.

    Endotoxins

    • A component (Lipid A) of lipopolysaccharide (LPS) in the outer membrane of Gram-negative bacteria.
    • Released when Gram-negative bacteria die.
    • High quantities of endotoxins can induce a strong immune response and lead to complications such as fever, chills, body aches, hypotension, and vomiting (symptoms of septic shock).
    • Endotoxemia is endotoxin in the blood stream, a potential consequence of infection and/or treatment use.

    Exotoxins

    • Soluble proteins that affect a wide range of cells.
    • Made by both Gram-positive and Gram-negative bacteria.
    • Classified based on organism or type of cell affected (e.g., neurotoxins, enterotoxins, hepatoxins, nephrotoxins).
    • Three main groups according to their mechanisms of action (Membrane-acting exotoxins, Membrane-damaging exotoxins, Intracellular AB toxins)

    Five Steps to Infection

    • Pathogens successfully infect a host following these five steps: entering the host, adherence to tissues, invasion and obtaining nutrients, replication while evading the immune system, and transmission to a new host.

    First, a Pathogen Must Enter a Host

    • Portals of entry are the sites pathogens use to enter the host.
    • Mucous membranes frequently serve as the primary portal of entry.

    Skin, Ocular, Otic, and Parenteral Entry

    • The largest body system, the integumentary system (skin, hair, nails, glands) blocks most microbes.
    • Some pathogens have developed virulence factors to overcome this defense, including getting in through abrasions, bites, injections, and surgical incisions.

    Respiratory Tract Entry

    • The respiratory tract is usually a common portal of entry because coughing and sneezing spread infectious agents in droplets.
    • Infectious agents can also be stirred into the air from dust or soil.

    Gastrointestinal (GI) Tract Entry

    • GI tract infection pathogens frequently use fecal-oral transmission.

    Urogenital Tract and Transplacental Entry

    • Sexually transmitted pathogens enter via the vaginal/cervical or urethral mucosa.
    • Some pathogens utilize transplacental entry (e.g., HIV).

    Second, a Pathogen Must Adhere to Host Tissues

    • Adhesion is often nonspecific.
    • Specific mechanisms may be used (via receptors) to adhere to the host cell.

    Adhesion Factors

    • Adhesins are virulence factors assisting in pathogen adhesion to host tissues.
    • These can be cell wall components, capsules, or plasma membrane molecules.

    Biofilms and Quorum Sensing

    • Bacteria develop biofilms on various surfaces.
    • Healthcare-associated biofilms frequently form on implanted devices or medical prosthetics.

    Third, a Pathogen Must Invade Tissues and Obtain Nutrients

    • Pathogens have several options after adhering:
      • Stay on the host cell surface,
      • Pass through cells to deeper tissues,
      • Enter cells as intracellular pathogens
    • Invasins help pathogens invade host tissues, including proteases, collagenases, and lipases.

    Invasins and Motility

    • Invasins allow pathogens to invade host tissues.
    • Motility helps a pathogen spread to another location within a host or to a new host.

    Tools to Obtain Nutrients: Siderophores and Extracellular Enzymes

    • Cellular pathogens require iron for survival.
    • Transferrin shuttles iron to tissues (and blood.)
    • Many bacteria produce siderophores that capture free iron from the host.
    • Extracellular enzymes from bacteria and fungi break down local nutrients to aid in getting nutrients. Examples include lipases (lipids) and proteases (proteins).

    Cytopathic Effects in the Host

    • Pathogens damage host cells, generating cytocidal (killing cells) and noncytocidal (damaging cells) effects.
    • Immune systems can also cause damage in the body as by-products of combating infections.

    Fourth, a Pathogen Must Evade Host Immune Defenses So It Can Replicate

    • Hiding and undermining defenses are critical to successful pathogen replication. Methods include intracellular lifestyle, latency, antibody masking/mimicry, antigenic variation, and interfering with phagocytosis, host immune responses.

    Hiding from Host Immune Defenses

    • Intracellular pathogens include viruses, some protozoans, and certain bacteria.
    • Latency is when pathogens lie dormant within a host, avoiding detection.
    • Antigenic masking/mimicry is when pathogens conceal their antigens to mask identification by the host's immune system.
    • Antigenic variation occurs when pathogens vary surface molecules to evade a rapid immune response.

    Undermining the Host Immune Response

    • Pathogens can interfere with phagocytosis, damaging phagocytic cells by releasing toxins, altering the host's immune response, or neutralising enzymes.

    Fifth, a Pathogen Must Be Transmitted to a New Host to Repeat the Cycle

    • The last step in establishing an infection is transmission to a new host.
    • Symptoms such as itchiness, sneezing, coughing, and diarrhea facilitates transmission to new hosts.

    Exiting the Host

    • Portals of exit are the routes pathogens use to leave their host, aiding further transmission.
    • Common examples include feces, urine, bodily fluids, wounds.

    Maintaining a Reservoir

    • A pathogen's reservoir is the environment or organism from which pathogens maintain their lifecycle.
    • Environmental niches such as soil, water can serve as reservoirs.
      • Microorganismal reservoirs include humans or animals.

    Safety and Health Care

    • Biosafety levels (BSL) help identify critical criteria for assigning pathogens to a BSL level.
    • There are four biosafety level categories: BSL-1, BSL-2, BSL-3, BSL-4.
    • Each BSL has associated specific personal protective equipment (PPE).

    Infection Control Practices

    • Most healthcare facilities have infection control teams.
    • Standard precautions and transmission precautions are essential for limiting infection risk for healthcare workers and patients.
      • Standard precautions treat all patients as potential sources of infection.
      • Transmission precautions are used when a specific infectious agent is suspected, including contact, droplet, or airborne precautions.

    Think Clinically: Be SMART About Cases

    • A case study of a patient with a hand wound that developed into a serious infection is presented.

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    Description

    Test your knowledge on the relationships between normal microbiota and hosts, the effects of dysbiosis, and the characteristics of pathogens. Explore how antibiotics can impact the microbiota and the immune system's role against pathogens. This quiz covers various aspects of host-microbe interactions.

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