Microbiology Fundamentals

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Questions and Answers

What is a key characteristic that differentiates prokaryotic cells from eukaryotic cells?

  • Prokaryotic cells have a nucleoid instead of a true nucleus. (correct)
  • Prokaryotic cells are always smaller than eukaryotic cells.
  • Prokaryotic cells contain multiple linear chromosomes.
  • Prokaryotic cells have larger ribosomes than eukaryotic cells.

Which scientist is known for developing aseptic techniques to control microbial contamination?

  • Charles Darwin
  • Louis Pasteur (correct)
  • Robert Koch
  • Antonie van Leeuwenhoek

What does binomial nomenclature specifically refer to?

  • The technique used to observe microbial movement.
  • The two-name system used for naming organisms. (correct)
  • The classification system for identifying viruses.
  • A method of determining the size of microorganisms.

What did Robert Koch contribute to the field of microbiology?

<p>He proposed the germ theory of disease. (D)</p> Signup and view all the answers

Which of the following is NOT a feature of prokaryotic cells?

<p>Presence of sterols in the cell membrane. (D)</p> Signup and view all the answers

Flashcards

What is physiology?

The study of the normal functions of living organisms and their parts.

What are microbes?

Tiny living organisms that can only be seen with a microscope. Examples include bacteria, viruses, fungi, and protozoa.

Who is Louis Pasteur?

Developed aseptic techniques, disproved spontaneous generation, and developed a rabies vaccine.

Who is Robert Koch?

Proposed the Germ Theory of Disease, which states that microbes cause infectious diseases.

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What is binomial nomenclature?

A two-part naming system used to give a unique scientific name to each organism. The first part is the genus (capitalized) and the second part is the species (lowercase).

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Study Notes

Physiology Definition

  • Physiology is the branch of biology studying the normal functions of living organisms and their components.

Key Figures in Microbiology

  • Antonie van Leeuwenhoek (1632-1723): First to observe living microbes using a single-lens microscope magnifying up to 300X.

  • Louis Pasteur (1822-1895): Demonstrated that microbes cause fermentation and spoilage, disproving spontaneous generation. Developed aseptic techniques and a rabies vaccine.

  • Robert Koch (1843-1910): Proposed the germ theory of disease, developed pure culture methods, and identified the causes of anthrax, tuberculosis, and cholera.

Microorganisms: Acellular and Cellular

  • Acellular:

  • Viruses

  • Viroids

  • Prions

  • Cellular:

  • Bacteria

  • Fungi

  • Protista (Protozoa and algae)

  • Helminths (worms)

Prokaryotes vs. Eukaryotes

Feature Prokaryotes Eukaryotes
Size Smaller Larger
Nucleus Absent Present
Organelles Absent Present
Chromosomes 1 circular Multiple, linear
Ribosomes Smaller (70s) Larger (80s)
Cell membranes Lack sterols (e.g., cholesterol) Sterols present

Scientific Nomenclature

  • Binomial nomenclature: Gives each microbe two names:
  • Genus (noun, capitalized)
  • Species (adjective, lowercase)
  • Both italicized or underlined (e.g., Staphylococcus aureus or S. aureus, Escherichia coli or E. coli)

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