Microbiology Fundamentals Quiz

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Questions and Answers

Which characteristic distinguishes viruses from bacteria?

  • Presence of DNA as the primary genetic material.
  • Ability to reproduce independently.
  • Eukaryotic cell structure.
  • Obligate intracellular parasitic nature. (correct)

A scientist discovers a new single-celled organism with a nucleus and membrane-bound organelles. To which field of microbiology does this organism most likely belong?

  • Mycology
  • Protozoology (correct)
  • Bacteriology
  • Virology

Which of the following is an example of a beneficial activity performed by microorganisms?

  • Inducing the development of emerging infectious diseases.
  • Producing toxins that contaminate food sources.
  • Causing antibiotic-resistant infections in humans.
  • Fixing atmospheric nitrogen into forms usable by plants. (correct)

An outbreak of a new disease, characterized by rapid spread and severe symptoms, is linked to a previously unknown virus. Which category does this disease most likely fall into?

<p>Emerging Infectious Disease (EID) (C)</p> Signup and view all the answers

In which of the following processes are genetically engineered microbes primarily utilized?

<p>Synthesizing human insulin for diabetes treatment. (C)</p> Signup and view all the answers

Which activity is LEAST likely to be attributed to normal flora in the human body?

<p>Directly causing severe invasive infections. (A)</p> Signup and view all the answers

What BEST describes the role of photosynthetic microbes in an ecosystem?

<p>Producing oxygen and energy, forming the base of aquatic food webs. (B)</p> Signup and view all the answers

How do bioremediating microbes contribute to environmental cleanup efforts?

<p>By directly neutralizing toxic pollutants through metabolic processes. (A)</p> Signup and view all the answers

What is the primary function of the rough endoplasmic reticulum (ER)?

<p>Protein synthesis. (D)</p> Signup and view all the answers

Which organelle is responsible for modifying, sorting, and packaging proteins and lipids for transport?

<p>Golgi Apparatus. (D)</p> Signup and view all the answers

What is the primary role of lysosomes within a cell?

<p>Digesting waste and pathogens. (B)</p> Signup and view all the answers

Which structural feature significantly increases the surface area for ATP production within mitochondria?

<p>The cristae. (B)</p> Signup and view all the answers

In what way do eukaryotic ribosomes differ from prokaryotic ribosomes?

<p>Eukaryotic ribosomes are larger. (C)</p> Signup and view all the answers

Which of the following is NOT a function of the nucleus?

<p>Synthesizing lipids. (C)</p> Signup and view all the answers

Considering the endosymbiotic theory, which of the following organelles is believed to have originated from an engulfed prokaryotic cell?

<p>Chloroplast. (C)</p> Signup and view all the answers

DNA gyrase, a type of topoisomerase, is crucial for regulating what specific aspect of DNA structure?

<p>Supercoiling. (C)</p> Signup and view all the answers

Which of the following statements accurately compares DNA and RNA?

<p>DNA contains deoxyribose sugar, while RNA contains ribose sugar. (D)</p> Signup and view all the answers

What is the role of ATP within a cell?

<p>To provide a source of energy. (A)</p> Signup and view all the answers

Which of these conversions requires knowledge of metric prefixes?

<p>Nanometers to millimeters. (A)</p> Signup and view all the answers

Which of the following correctly describes the relationship between wavelength and resolution in microscopy?

<p>Shorter wavelengths provide higher resolution. (C)</p> Signup and view all the answers

A researcher is studying live bacteria that are difficult to stain and nearly invisible under a brightfield microscope. Which microscopy technique would be most suitable for this purpose?

<p>Darkfield microscopy. (C)</p> Signup and view all the answers

A scientist is using fluorescence microscopy to observe the location of a specific protein within a cell. What is an essential requirement for this technique?

<p>The protein must be tagged with a fluorescent molecule. (B)</p> Signup and view all the answers

If a molecule of DNA has 20% Adenine, what percentage of Cytosine would you expect it to have?

<p>30% (C)</p> Signup and view all the answers

What is the purpose of using oil with the 1000X lens in light microscopy?

<p>To reduce refraction and improve resolution. (D)</p> Signup and view all the answers

In a dichotomous key, a specimen is identified as Struthio camelus after determining it has no feathers at step 1 and cannot fly at step 2. What correction is needed to improve the key?

<p>Add more characteristics to differentiate specimens better. (B)</p> Signup and view all the answers

If a bacterium is analyzed using membrane fatty acid analysis, what information is gained?

<p>The types and quantities of fatty acids in its membrane. (A)</p> Signup and view all the answers

Why is rRNA analysis a valuable tool for bacterial classification?

<p>rRNA genes are highly conserved, allowing for broad classification. (C)</p> Signup and view all the answers

Following PCR amplification of a bacterial sample, gel electrophoresis reveals several bands of varying sizes. What does this indicate about the sample?

<p>The sample contains multiple bacterial species or non-specific amplification occurred. (C)</p> Signup and view all the answers

How do DNA chips, or microarray tests, identify bacteria?

<p>By detecting the hybridization of bacterial genes to small DNA fragments on the chip. (C)</p> Signup and view all the answers

What information does GC content provide about a bacterial species?

<p>The percentage of guanine and cytosine in its DNA. (C)</p> Signup and view all the answers

If a bacterium is resistant to a particular bacteriophage, what does this imply for phage susceptibility testing?

<p>The bacterium does not possess the surface receptors required for that phage to attach and infect. (A)</p> Signup and view all the answers

How are anabolism and catabolism related through ATP?

<p>Catabolism produces ATP, which is then used in anabolism. (A)</p> Signup and view all the answers

Which of the following best describes a biochemical pathway?

<p>A multi-step series of sequential reactions where the product of one reaction becomes the substrate for the next. (C)</p> Signup and view all the answers

The active site of an enzyme is crucial for its function because it:

<p>Binds to the substrate and facilitates a chemical reaction. (C)</p> Signup and view all the answers

Which of the following correctly matches a term with its role in enzyme function?

<p>Substrate: The molecule that is chemically modified by the enzyme. (C)</p> Signup and view all the answers

How does increasing temperature typically affect enzyme activity, and what is a potential consequence of extreme temperatures?

<p>Increases activity up to an optimal point; extreme temperatures can cause denaturation. (D)</p> Signup and view all the answers

Why does enzyme activity plateau at high substrate concentrations?

<p>The enzyme becomes saturated, and all active sites are occupied. (A)</p> Signup and view all the answers

In competitive inhibition, how can the effect of the inhibitor be overcome?

<p>By increasing the concentration of the substrate. (C)</p> Signup and view all the answers

Allosteric inhibitors affect enzyme activity by:

<p>Binding to a site other than the active site, inducing a conformational change that reduces active site effectiveness. (D)</p> Signup and view all the answers

Which of the following is an example of feedback inhibition in enzyme regulation?

<p>The final product of a metabolic pathway inhibits an enzyme earlier in the same pathway. (C)</p> Signup and view all the answers

In fluorescence microscopy, what is the primary purpose of using fluorescent dyes?

<p>To specifically label antibodies or pathogens for identification. (D)</p> Signup and view all the answers

What information does Scanning Electron Microscopy (SEM) provide about a sample?

<p>Detailed surface details in 3D. (C)</p> Signup and view all the answers

Which type of staining is used to distinguish different types of bacteria based on their cell wall structure?

<p>Differential stain (C)</p> Signup and view all the answers

Why do capsules appear colorless when using a capsule stain?

<p>The polysaccharides in capsules repel dyes. (D)</p> Signup and view all the answers

A microbiologist discovers a new unicellular organism. Initial observations reveal that the cell lacks a nucleus and any membrane-bound organelles. What type of cell is this most likely to be?

<p>Prokaryotic cell (C)</p> Signup and view all the answers

Under a microscope, a bacterial sample shows spherical cells arranged in grape-like clusters. Which term accurately describes this arrangement?

<p>Staphylococci (D)</p> Signup and view all the answers

Why are biofilms particularly problematic in medical settings?

<p>They are mixed communities of bacteria and are difficult to disinfect. (A)</p> Signup and view all the answers

Flashcards

Nucleic Acids

DNA and RNA that contain genetic information and are made of CHONP.

Purines vs Pyrimidines

Purines (A, G) have double rings, while Pyrimidines (C, T, U) have single rings.

Base Pairing in DNA

Adenine pairs with Thymine (2 hydrogen bonds) and Cytosine pairs with Guanine (3 hydrogen bonds).

Base Pairing in RNA

Adenine pairs with Uracil and Cytosine pairs with Guanine.

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Function of DNA

DNA stores and transfers genetic information; serves as a template for mRNA.

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ATP

Adenosine Triphosphate, the main energy carrier in cells.

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Refractive Index

A measure of how much light bends when entering a medium.

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Dark Field Microscopy

A technique used to observe live microorganisms that are not visible in standard methods.

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Fluorescence microscopy

A technique for marking antibodies or pathogens with dyes for diagnostics.

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Transmission electron microscopy (TEM)

Technique where electrons pass through a sample to view internal structures in 2D.

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Scanning Electron microscopy (SEM)

Technique where electrons bounce off a sample’s surface to show 3D details.

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Basic dyes

Dyes with a positive charge that stain cell structures.

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Acidic dyes

Dyes with a negative charge that stain the background, not the cells.

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Capsules stain

Specialty stain that makes capsules appear colorless due to dye repulsion.

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Biofilm

A mixed community of bacteria on surfaces that are difficult to disinfect.

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Prokaryotic cells

Cells without a membrane-bound nucleus or organelles, usually 0.2 - 2 microns.

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Virology

The study of viruses, which are very small, obligate intracellular parasites.

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Bacteriology

The study of bacteria, which are small, self-sufficient prokaryotic cells with DNA.

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Protozoology

The study of protozoans, which are large eukaryotic cells with true nuclei and organelles.

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Mycology

The study of fungi, which can be unicellular (yeasts) or multicellular (molds, mushrooms), and are eukaryotic.

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Emerging Infectious Diseases (EIDs)

New or re-emerging diseases caused by microbial evolution, human activity, or environmental changes.

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Normal Flora

Beneficial microbes that are usually non-pathogenic and support digestion, vitamin production, and competition with pathogens.

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Bioremediating Microbes

Microorganisms that help clean up environmental issues, like sewage or toxic spills.

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Pathogen

An organism that causes disease, including viruses, bacteria, fungi, and parasites.

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Nucleus

Stores genetic material (DNA), controls cell activities, and regulates gene expression.

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Endoplasmic Reticulum (ER)

Synthesizes proteins (rough ER) and lipids (smooth ER), transports molecules, and detoxifies chemicals.

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Rough ER

Studded with ribosomes, involved in protein synthesis.

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Smooth ER

Lacks ribosomes, involved in lipid synthesis and detoxification.

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Golgi Apparatus

Modifies, sorts, and packages proteins and lipids for transport.

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Lysosomes

Digest and break down waste, pathogens, and damaged organelles using hydrolytic enzymes.

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Mitochondria

Generate ATP through cellular respiration, providing energy for the cell.

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Endosymbiosis

A type of symbiotic relationship where one organism lives inside another's cells.

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Metabolic Pathways

A series of sequential biochemical reactions converting substrates into products.

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Active Site

The specific region on an enzyme where the substrate binds and the reaction occurs.

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Substrate

The reactant molecule that binds to an enzyme's active site and undergoes a reaction.

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Product

The final molecule(s) formed after an enzyme catalyzes a reaction.

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Coenzyme

A non-protein organic molecule that assists enzymes, often derived from vitamins.

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Cofactor

A non-protein helper, such as a metal ion, that enhances enzyme activity.

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Factors Affecting Enzyme Activity

pH, temperature, and substrate concentration can enhance or reduce enzyme activity.

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Types of Enzyme Inhibition

Competitive, allosteric, and feedback inhibition affect enzyme function differently.

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DNA Sequence Analysis

Uses genetic sequencing to identify bacteria by comparing DNA sequences to known databases.

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rRNA Analysis

Examines ribosomal RNA genes to classify bacteria, as these sequences are highly conserved.

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GC Content

Measures the percentage of guanine and cytosine in bacterial DNA, aiding species differentiation.

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DNA Chips

Uses small DNA fragments on a chip to detect the presence of bacterial genes through hybridization.

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DNA Fingerprinting

Analyzes genetic variations with techniques like Restriction Fragment Length Polymorphism (RFLP) to distinguish strains.

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Polymerase Chain Reaction (PCR)

Amplifies specific DNA sequences to detect bacterial DNA in small samples.

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Membrane Fatty Acid Analysis

Identifies bacteria by analyzing the composition of their fatty acids, which vary by species.

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Fluorescent in situ Hybridization (FISH)

Uses fluorescent probes that bind to specific bacterial DNA sequences, allowing visualization under a fluorescence microscope.

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Study Notes

Study Guide Summary

  • General Microbiology Study Guide Chapter 1 (Introduction to Microbiology): This chapter covers major contributors to microbiology, definitions, and the types of organisms studied.
  • Contributions: Leeuwenhoek (first bacteria observation, microscope), Koch (proof of disease-causing microbes), Pasteur (disproved spontaneous generation), Semmelweis (handwashing), Jenner (smallpox vaccine), Ehrlich (chemotherapy).
  • Organism Types: Virology (study of viruses), Bacteriology (study of bacteria - prokaryotic), Protozoology (study of protozoans - eukaryotic), Mycology (study of fungi - eukaryotic), Parasitology (study of worms - eukaryotic).
  • Emerging Infectious Diseases (EIDs): New or re-emerging diseases caused by microbial evolution, human activity, or environmental changes. Examples listed in text.
  • Microorganism Benefits: Photosynthetic microbes, nitrogen fixing microbes, normal flora (probiotics), food/beverage/drug producers, and bioremediation microbes.

Chemical Principles (Chapter 2)

  • Key Elements: Oxygen (O), Carbon (C), Hydrogen (H), Nitrogen (N)
  • Chemical Bonds: Ionic (transfer of electrons), Covalent (sharing of electrons), Hydrogen (weak attraction)
  • Chemical Reactions: Synthesis (anabolic) - building larger molecules, Decomposition (catabolic) - breaking down larger molecules.
  • Electron Shells: Affect bond formation as atoms share, gain, or lose electrons to form a stable structure.
  • Polar Solvents: Water (Hâ‚‚O) is a polar solvent because of uneven charge distribution that makes it a good solvent for polar molecules.
  • pH Scale: Acidic (<7), Neutral (=7), Basic(>7)
  • Functional Groups: Hydroxyl, Amino, Carboxyl, Phosphate (structures and functions as described in the text).

Microscopy (Chapter 3)

  • Metric System: Standard units of length measurement (pico, nano, micro, milli, centi, and kilo).
  • Microscope Resolution Limits: Unaided eye (>200μm), Light microscope (10nm-200nm), Electron microscope (10pm-100pm)
  • Refraction and Refractive Index: Light bending as it passes through different media.
  • Dark Field vs Bright Field Microscopy: Methods to visualize microbes and their structures. Dark field used to visualize live specimens or specimens that fluoresce.
  • Fluorescence Microscopy: Technique to identify microbes and structures.

Prokaryotic and Eukaryotic Cells (Chapter 4)

  • Prokaryotic vs. Eukaryotic: Key differences (nucleus, organelles).
  • Bacterial Shapes & Arrangements: Cocci (spherical), Bacilli (rod-shaped), Spiral (curved)
  • Bacterial Cell Walls: Gram-positive (thick peptidoglycan layer), Gram-negative (thin peptidoglycan layer with an outer membrane)
  • Cell Membrane Structure: Bilayer structure with integral and peripheral proteins.
  • Transport Across Membranes: Simple diffusion, osmosis, facilitated diffusion, active transport.

Microbial Classification (Chapter10)

  • Taxonomy: Science of classifying organisms.
  • Phylogenetic (Systematics): Use evolutionary relationships for classification.

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