Microbiology Disinfection Methods Quiz
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Questions and Answers

What type of radiation is considered a form of cold sterilization?

  • Ultraviolet Rays
  • Beta Rays
  • Infrared Rays
  • Gamma Rays (correct)

Which of the following is NOT a characteristic of HEPA filters?

  • Works only for liquid applications (correct)
  • Traps small, harmful particles
  • Requires frequent replacement
  • Uses electrostatic attraction for filtration

What is the primary action of chemical agents like chlorine dioxide gas?

  • Inhibits protein synthesis
  • Releases free radicals
  • Decomposes organic matter
  • Sterilizes medical equipment and surfaces (correct)

Which chemical agent is an example of a phenolic compound used in disinfection?

<p>Triclosan (D)</p> Signup and view all the answers

Which factor does NOT affect the germicidal activity of chemical agents?

<p>Color of the liquid solution (C)</p> Signup and view all the answers

What is the standard mode of action for nonionizing radiation like ultraviolet rays?

<p>Forms pyrimidine dimers (A)</p> Signup and view all the answers

What is an important consideration when using liquid disinfectants such as iodine?

<p>They can damage human tissues (B)</p> Signup and view all the answers

What is the primary function of filters in liquid applications?

<p>To remove specific contaminants based on pore size (C)</p> Signup and view all the answers

What is the primary function of Betapropiolactone?

<p>To inactivate viruses (B)</p> Signup and view all the answers

What action do quaternary ammonium compounds commonly perform?

<p>They serve as detergents and disinfectants (C)</p> Signup and view all the answers

Which group of antimicrobial agents is known for blocking cell wall synthesis?

<p>Penicillin and its relatives (B)</p> Signup and view all the answers

What is the main mechanism of action for rifampin in antimicrobial therapy?

<p>To block RNA synthesis (C)</p> Signup and view all the answers

Which of the following compounds exerts lethality upon microorganisms in extremely small amounts?

<p>Oligodynamic compounds (C)</p> Signup and view all the answers

What is the role of aminoglycosides in antimicrobial therapy?

<p>Interfere with protein synthesis (C)</p> Signup and view all the answers

Which drug is known as an antimetabolite that blocks metabolic pathways?

<p>Trimethoprim (A)</p> Signup and view all the answers

What type of action do phenol compounds typically exhibit in antimicrobial applications?

<p>Cell membrane disruption (B)</p> Signup and view all the answers

What is the primary purpose of a germicide?

<p>To kill pathogens (C)</p> Signup and view all the answers

Which of the following methods is most effective for sterilization using moist heat?

<p>Autoclaving (D)</p> Signup and view all the answers

How does dry heat sterilization primarily differ from moist heat sterilization?

<p>Dry heat requires longer exposure times (B)</p> Signup and view all the answers

What is the main effect of refrigeration on microorganisms?

<p>It inhibits their growth (C)</p> Signup and view all the answers

In which scenario would dry heat ovens typically be used for sterilization?

<p>Sterilizing metal and glass instruments (B)</p> Signup and view all the answers

Which of the following is a key factor that affects the death rate of microorganisms?

<p>Activity of the antimicrobial agent (C)</p> Signup and view all the answers

What is the principle of pasteurization?

<p>To increase the temperature for a short duration (A)</p> Signup and view all the answers

What is the effect of desiccation on microorganisms?

<p>It inhibits growth by removing water (D)</p> Signup and view all the answers

Flashcards

Lyophilization

A method of preserving substances by freezing and removing water using sublimation.

Ionizing Radiation

High-energy radiation (gamma rays, X-rays) that causes significant damage to microbes' DNA, leading to sterilization.

Nonionizing Radiation

Lower-energy radiation (e.g., UV rays) that damages DNA but not as severely as ionizing radiation.

Filtration

A physical method to remove microbes by using a porous barrier.

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Disinfectants

Chemical agents that kill or inactivate pathogens on inanimate objects.

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Sterilants

Chemical agents that eliminate all forms of microbial life, including endospores, on inanimate objects only.

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Chlorine Dioxide

A gas used as a sterilant for surfaces, medical equipment, and tools.

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HEPA Filters

High-efficiency particulate air filters used to remove small particles from air, (e.g., dust, pollen)

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Sterilization

A process that kills all forms of life, including endospores and viruses.

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Bacteriocidal

Kills bacteria.

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Disinfection

The process of killing or removing pathogens from inanimate objects.

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Autoclave

A device that sterilizes using steam under pressure.

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Pasteurization

A process of disinfection used to eliminate pathogens from beverages.

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Moist Heat sterilization

Uses heat and water to kill microbes.

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Dry Heat Sterilization

Incineration or oven to kill and remove microbes.

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High Temperature Short Time (HTST)

A method of pasteurization involving a brief exposure to a high temperature.

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Betapropiolactone function

Modifies nucleic acid structure to inactivate viruses.

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Quaternary Ammonium Compounds (Quats)

Chemicals found in many cleaning and personal care products (e.g., shampoos, disinfectants).

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Oligodynamic Action

Small amounts of certain metals showing antimicrobial effects.

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Antimicrobial drugs

Substances that combat or stop the growth of microorganisms.

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Selective Toxicity

An antibiotic targeting a microbe without harming the host.

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Cell Wall Inhibitors

Antibiotics that target the microbe's cell walls.

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Fluoroquinolones

Antibiotics that affect DNA synthesis in microbes.

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Protein Synthesis Inhibitors

Antibiotics halting the production of proteins in microbes.

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