Microbiology Course Code 30203321 Topic 5 Chapter 9 Quiz
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Questions and Answers

Match the following actions of microbial control agents with their effects:

Alteration of membrane permeability = Damage to nucleic acids Damage to proteins = Damage to nucleic acids Damage to nucleic acids = Alteration of membrane permeability

Match the following physical methods of microbial control with their descriptions:

Heat = Lowest temperature at which all cells in a culture are killed in 10 min. Thermal death point (TDP) = Heat

Match the following methods of microbial control with their descriptions:

Thermal death time (TDT) = Time to kill all cells in a culture Decimal reduction time (DRT) = Minutes to kill 90% of a population at a given temperature Autoclave = Sterilization method using steam under pressure Filtration = Method that removes microbes

Match the following physical methods of microbial control with their effects:

<p>Moist heat = Denatures proteins Dry Heat = Kills by oxidation Low temperature = Inhibits microbial growth High pressure = Denatures proteins</p> Signup and view all the answers

Match the following chemical disinfectants with their effects:

<p>Phenol = Disrupts plasma membranes Halogens (Iodine, Chlorine) = Act as oxidizing agents Alcohols (Ethanol, isopropanol) = Denature proteins, dissolve lipids Heavy Metals (Ag, Hg, Cu) = Exhibit oligodynamic action</p> Signup and view all the answers

Match the following types of disinfectants with their descriptions:

<p>Surfactants = Sanitizing, bactericidal; denature proteins and disrupt plasma membrane Chemical Food Preservatives = Inhibit metabolism; control molds and bacteria in foods and cosmetics Aldehydes = Inactivate proteins by cross-linking with functional groups (–NH2, –OH, –COOH, —SH) Antibiotics = Prevent spoilage</p> Signup and view all the answers

Match the following physical methods of microbial control with their equivalent treatments:

<p>Autoclave = 121˚C, 15 min Hot-air sterilization = 170˚C, 2 hr Refrigeration = Inhibits microbial growth Lyophilization = Prevents metabolism</p> Signup and view all the answers

Match the following chemical disinfectants with their types:

<p>Phenolic (Lysol, Bisphenols) = Disrupt plasma membranes Biguanides (Chlorhexidine) = Sanitizing, bactericidal; denature proteins and disrupt plasma membrane Organic Acids (Sorbic acid, benzoic acid) = Inhibit metabolism; control molds and bacteria in foods and cosmetics Nitrite = Prevents endospore germination</p> Signup and view all the answers

Match the following physical methods of microbial control with their primary effects:

<p>Heat = Denatures proteins Radiation = Damages DNA Low temperature = Inhibits microbial growth High pressure = Denatures proteins</p> Signup and view all the answers

Match the following chemical disinfectants with their mechanism of action:

<p>Halogens (Iodine, Chlorine) = Oxidizing agents Alcohols (Ethanol, isopropanol) = Denature proteins, dissolve lipids Heavy Metals (Ag, Hg, Cu) = Exhibit oligodynamic action Surface-Active Agents or Surfactants = Sanitizing; denature proteins and disrupt plasma membrane</p> Signup and view all the answers

Match the following methods of microbial control with their specific applications:

<p>Filtration = Removes microbes Desiccation = Prevents metabolism Osmotic pressure = Causes plasmolysis Nonionizing radiation (UV) = Kills microbes</p> Signup and view all the answers

Match the following chemical disinfectants with their use in evaluating a disinfectant:

<p>Phenol = Used in the disk-diffusion method Aldehydes = Inactivate proteins by cross-linking with functional groups (–NH2, –OH, –COOH, —SH) Antibiotics = Used for testing effective disinfection methods Surface-Active Agents or Surfactants = Degerming</p> Signup and view all the answers

What is the primary difference between catabolism and anabolism?

<p>Catabolism releases energy, while anabolism uses energy.</p> Signup and view all the answers

What determines the metabolic pathways in a cell?

<p>Enzymes</p> Signup and view all the answers

According to the laws of thermodynamics, what does the first law state?

<p>Energy changes state from one form to another.</p> Signup and view all the answers

In a spontaneous (exergonic) reaction, what is the standard free energy change (ΔGo’)?

<p>Negative</p> Signup and view all the answers

What is the primary function of photosynthesis in microbial metabolism?

<p>Produce glucose using light energy</p> Signup and view all the answers

Which factor determines the change in free energy during a chemical reaction?

<p>Reaction heat (Enthalpy)</p> Signup and view all the answers

What is the sum of the chemical reactions in an organism known as?

<p>Metabolism</p> Signup and view all the answers

Which type of reaction is involved in the energy-releasing processes?

<p>Catabolic reactions</p> Signup and view all the answers

What determines the metabolic pathways in a cell?

<p>Enzymes</p> Signup and view all the answers

Which law of thermodynamics states that energy is neither created nor destroyed, but simply changes state?

<p>First law</p> Signup and view all the answers

What does the second law of thermodynamics state about the order of a system?

<p>It becomes more random</p> Signup and view all the answers

What is the equation for the change in free energy during a chemical reaction?

<p>$$\Delta G = \Delta H - T \cdot \Delta S$$</p> Signup and view all the answers

Which type of reaction is indicated by a negative ΔH?

<p>Exergonic reaction</p> Signup and view all the answers

What is the function of electron carriers like NADH in biological systems?

<p>Supply reducing power in catabolic reactions</p> Signup and view all the answers

Which enzyme type is involved in the rearangement of atoms in a molecule?

<p>Isomerase</p> Signup and view all the answers

What is the primary function of coenzyme Q (Ubiquinone) in biological systems?

<p>Release energy from the transfer of electrons</p> Signup and view all the answers

In which type of reaction is ATP generated by the phosphorylation of ADP?

<p>Substrate-level phosphorylation</p> Signup and view all the answers

What is the role of competitive inhibitors in enzyme activity?

<p>Bind to the active site and prevent substrate binding</p> Signup and view all the answers

What effect does feedback inhibition have on metabolic pathways?

<p>Prevents the buildup of excessive product</p> Signup and view all the answers

What characterizes an oxidation reaction in biological systems?

<p>Gain of electrons</p> Signup and view all the answers

Which process utilizes energy released from the transfer of electrons to generate ATP?

<p>'Chemiosmosis'</p> Signup and view all the answers

What can denature enzymes?

<p>Temperature, pH and substrate concentration.</p> Signup and view all the answers

Which type of reaction involves the removal of atoms without hydrolysis?

<p>Lyase</p> Signup and view all the answers

What is the function of electron carriers like NADH and NADPH?

<p>Control the release of energy for work.</p> Signup and view all the answers

In which type of reaction are the electrons often associated with hydrogen atoms?

<p>Redox reaction</p> Signup and view all the answers

Which factor influences enzyme activity by binding to the active site and competing with the substrate?

<p>Competitive inhibitor</p> Signup and view all the answers

What is the role of ATP in the context of metabolism regulation?

<p>ATP supplies reducing power in anabolic reactions.</p> Signup and view all the answers

What is the function of oxidoreductase enzymes?

<p>Oxidation-reduction reactions.</p> Signup and view all the answers

Which type of reaction involves the transfer of functional groups?

<p>Transferase</p> Signup and view all the answers

Which factor influences enzyme activity by binding to a site other than the active site, changing the shape of the enzyme, and reducing its activity?

<p>Noncompetitive inhibitor</p> Signup and view all the answers

In which type of reaction does oxidation involve the removal of electrons?

<p>Redox reaction</p> Signup and view all the answers

Which type of reaction involves the joining of molecules and uses ATP?

<p>Ligase</p> Signup and view all the answers

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