Microbiology and Parasitology: History Overview

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Questions and Answers

Who is known as the Father of Modern Microbiology?

  • Louis Pasteur (correct)
  • Joseph Lister
  • Edward Jenner
  • Robert Koch

Joseph Lister is credited with the development of the first vaccine.

False (B)

What year did Edward Jenner develop the smallpox vaccine?

1796

Robert Koch introduced the use of ___ media for the culture of bacteria.

<p>solid</p> Signup and view all the answers

Which of the following diseases did Louis Pasteur develop a vaccine for?

<p>Rabies (D)</p> Signup and view all the answers

Match the following individuals to their contributions:

<p>Louis Pasteur = Germ theory of disease Edward Jenner = First vaccine for smallpox Joseph Lister = Antiseptic surgery Robert Koch = Pure culture techniques</p> Signup and view all the answers

Koch's phenomenon describes a hypersensitivity reaction to tuberculosis bacilli.

<p>True (A)</p> Signup and view all the answers

What technique did Louis Pasteur develop to preserve food?

<p>Pasteurization</p> Signup and view all the answers

What contributed to the development of microbiology as a scientific discipline in the late 1800s?

<p>Improved microscopes and new laboratory techniques (A)</p> Signup and view all the answers

Prof. Charles E. Sedillot was the first person to observe microorganisms.

<p>False (B)</p> Signup and view all the answers

Which microorganism did Martinus W. Beijerinck isolate that is responsible for fixing atmospheric nitrogen?

<p>Azotobacter</p> Signup and view all the answers

The term ______ was given by Prof. Charles E. Sedillot.

<p>microbe</p> Signup and view all the answers

Match the following scientists with their contributions:

<p>Louis Pasteur = Demonstrated roles of yeasts and bacteria in fermentation Antonie van Leeuwenhoek = First to observe microorganisms Martinus W. Beijerinck = Isolated nitrogen-fixing microorganisms Charles E. Sedillot = Coined the term 'microbe'</p> Signup and view all the answers

When did Louis Pasteur publish his papers regarding yeasts and bacteria?

<p>1857-1860 (D)</p> Signup and view all the answers

Microbes play a significant role in human affairs according to scientists after the 1800s.

<p>True (A)</p> Signup and view all the answers

How long ago did the first microbe (prokaryote) evolve?

<p>3.6 billion years ago</p> Signup and view all the answers

Which type of microbes lack a membrane-bound nucleus?

<p>Prokaryotes (D)</p> Signup and view all the answers

Aquatic microenvironments occupy less than 50% of the Earth's surface.

<p>False (B)</p> Signup and view all the answers

What is the study of fungi called?

<p>Mycology</p> Signup and view all the answers

The study of single-celled eukaryotes is known as ______.

<p>Protozoology</p> Signup and view all the answers

Match the following branches of microbiology with their focus:

<p>Immunology = Study of the immune system Bacteriology = Study of bacteria Virology = Study of viruses Parasitology = Study of parasites</p> Signup and view all the answers

Which of the following is NOT a characteristic of terrestrial microenvironments?

<p>Higher availability of water (A)</p> Signup and view all the answers

Microbial diversity is solely limited to bacteria and viruses.

<p>False (B)</p> Signup and view all the answers

What role do aquatic microbiota play in the ecosystem?

<p>Primary producers and primary consumers</p> Signup and view all the answers

What type of relationship benefits both partners?

<p>Mutualism (D)</p> Signup and view all the answers

Amensalism occurs when one individual benefits and the other is harmed.

<p>False (B)</p> Signup and view all the answers

What is the term for a relationship where one organism lives on or in another organism?

<p>Parasitism</p> Signup and view all the answers

In a mutualistic relationship, both partners ______.

<p>benefit</p> Signup and view all the answers

Which term describes the relationship where microorganisms compete for limited nutrients?

<p>Competition (C)</p> Signup and view all the answers

Match the type of interaction with its description:

<p>Mutualism = Both partners benefit Parasitism = One organism benefits at the expense of another Competition = Organisms vie for limited resources Amensalism = One organism is harmed while the other is unaffected</p> Signup and view all the answers

Competition always leads to the extinction of one of the organisms involved.

<p>False (B)</p> Signup and view all the answers

What are the negative associations that encompass antagonism, competition, and parasitism?

<p>Negative associations</p> Signup and view all the answers

What is the formula to calculate total magnification in microscopy?

<p>Objective lens power x ocular lens power (B)</p> Signup and view all the answers

Resolution refers to the ability to distinguish two points that are far apart.

<p>False (B)</p> Signup and view all the answers

What was the resolving power of Leeuwenhoek's microscopes?

<p>1 mm</p> Signup and view all the answers

Modern microscopes have a resolving power of as close as _____ mm.

<p>0.2</p> Signup and view all the answers

Match the following terms with their definitions:

<p>Magnification = Increasing the apparent size of an object Resolution = Ability to distinguish two points close together Contrast = Difference in intensity between objects or background Numerical Aperture = Lens ability to gather light</p> Signup and view all the answers

Which type of radiation do modern microscopes use to achieve better resolution?

<p>Blue light (D)</p> Signup and view all the answers

Why is contrast important in microscopy?

<p>It helps in determining resolution.</p> Signup and view all the answers

Most microorganisms are highly colored, making them easy to see under a microscope.

<p>False (B)</p> Signup and view all the answers

Which staining solution is NOT mentioned as being useful in electron microscopy?

<p>Eosin (C)</p> Signup and view all the answers

Aseptic techniques are only necessary for preventing contamination in microbiology labs.

<p>False (B)</p> Signup and view all the answers

What is the purpose of staining solutions in histology, virology, and pathology?

<p>To enhance contrast in specimens for better study and diagnosis.</p> Signup and view all the answers

The primary goal of aseptic techniques is to prevent __________ of cultures and solutions.

<p>contamination</p> Signup and view all the answers

Match the following aseptic techniques with their correct description:

<p>Cleaning lab surfaces = Removes organic material and reduces microbial presence Limiting culture exposure = Minimizes risk of airborne contamination Sterilizing inoculating loops = Prevents introducing pathogens into cultures Keeping petri dishes closed = Protects cultures from environmental contaminants</p> Signup and view all the answers

What should be avoided while working with cultures or sterile instruments?

<p>Breathing on them (A)</p> Signup and view all the answers

A completely sterile working environment is achievable in a laboratory setting.

<p>False (B)</p> Signup and view all the answers

Name one common procedure to reduce the risk of culture contamination.

<p>Cleaning and disinfecting lab surfaces.</p> Signup and view all the answers

Flashcards

Fermentation

The process of inhibiting the growth of microorganisms, often for the purpose of preserving food.

Pasteurization

A method of killing microorganisms in liquids, typically milk, by heating it to a specific temperature for a set time.

Sterilization

A technique using heat to kill microorganisms on surfaces or in liquids.

Hot Air Oven Sterilization

The process of exposing a substance to a high-heat environment for a set time to kill microorganisms.

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Autoclave Sterilization

A method of sterilization using steam at high pressure and temperature to kill microorganisms.

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Disinfectant

Any substance that destroys microorganisms.

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Antiseptic

A substance that inhibits the growth of microorganisms.

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Germ Theory of Disease

The theory that states that diseases are caused by microorganisms.

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Early views on microbes

Scientists in the 1700s viewed microbes as unimportant curiosities, but in the late 1800s, a shift towards experimental evidence propelled microbiology as a science.

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Who coined "microbe"?

The term "microbe" was coined by Professor Charles E. Sedillot who recognized their existence and action while studying post-operative infections.

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Pasteur's contributions to microbiology

Louis Pasteur's research demonstrated specific activities of yeasts and bacteria in fermentation, leading to discoveries about wine diseases and pasteurization.

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Beijerinck's contribution to microbial ecology

Martinus W. Beijerinck made significant contributions to microbial ecology by highlighting the role of microbes in plant nitrogen fixation.

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Evolution of microbes

Microbes have evolved for billions of years, utilizing a vast range of energy sources and thriving in diverse habitats.

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Microbial origins of life

Essential biochemical processes of life likely originated from microbial ancestors.

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Van Leeuwenhoek and the first observations

Antonie Philips van Leeuwenhoek, the first scientist to observe bacteria and other microorganisms, called them "animalcules."

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Key developments in microbiology

The development of improved microscopes, laboratory techniques, and a thirst for knowledge led to the advancement of microbiology in the late 1800s.

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What is microbiology?

The study of microscopic organisms including bacteria, viruses, fungi, and protists.

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What are prokaryotes?

These organisms lack a nucleus and include bacteria, viruses, and archaea.

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What are eukaryotes?

These organisms have a nucleus and include algae, fungi, protozoa, and microscopic animals.

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What is bacteriology?

The branch of microbiology that studies bacteria.

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What is virology?

The branch of microbiology that studies viruses.

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What is mycology?

The branch of microbiology that studies fungi.

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What is parasitology?

The branch of microbiology that studies parasites.

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What is a terrestrial microenvironment?

Microbial communities and their environment on landmasses, influenced by water availability.

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Resolution

The ability of a microscope to distinguish two points that are close together.

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Contrast

The difference in intensity between two objects or between an object and its background.

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What determines a microscope's resolution?

The wavelength of the light used and the numerical aperture of the lens.

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Numerical Aperture

The ability of a lens to gather light.

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Why do modern microscopes have better resolution than Leeuwenhoek's?

Modern microscopes use shorter wavelength radiation, such as blue light or electron beams, and they have lenses with larger numerical apertures.

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How much better is the resolution of modern microscopes than Leeuwenhoek's?

Modern microscopes have a greater resolving power than Leeuwenhoek's, allowing us to see objects much closer together.

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Why is contrast important in microscopy?

Most microorganisms are colorless and have little contrast when viewed under a microscope.

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What is the numerical aperture of a lens?

The ability of a lens to gather and focus light.

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Aseptic Technique

A set of procedures used to minimize contamination in a laboratory setting.

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Sterilization and Disinfection

Techniques used to kill or inhibit the growth of microorganisms on surfaces or in liquids.

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Microbiology

The study of microscopic organisms like bacteria, viruses, fungi, and protists.

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Staining in Microscopy

Staining solutions are used to enhance the visibility of microorganisms under a microscope.

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Microorganisms

Living organisms that are not visible to the naked eye.

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Preventing Contamination

The practice of preventing contamination of cultures, media, and other laboratory materials.

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The Importance of Sterility

Maintaining a sterile working environment is essential for accurate and meaningful experimentation.

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Identifying Microbes with Staining

The use of specific dyes to identify and differentiate microorganisms.

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Mutualism

A relationship where both partners benefit from the interaction, like cleaning stations on coral reefs where cleaner fish eat parasites off other fish, helping both fish.

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Parasitism

This occurs when one organism benefits at the expense of another, often without killing it directly. For example, a parasite like a tapeworm living in a human's gut.

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Amensalism

A type of interaction where one organism is negatively affected, while the other remains unharmed. Think of a large tree shading out smaller plants.

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Competition between Microorganisms

Microorganisms compete for limited resources like nutrients and space. The microbe that wins gets more resources, while other microbes may lose out.

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Antagonism

This refers to an antagonistic relationship between microorganisms, where one organism inhibits the growth or survival of the other. It often involves molecules that produce harmful effects.

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Antibiosis

A special type of antagonism where one organism produces substances that inhibit the growth or survival of another. This is often used to discover and develop new antimicrobial drugs.

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Syntropism

A type of relationship where two organisms benefit from the interaction, but one organism's waste product is used as an energy source by the second organism. Methane-producing bacteria and protozoans in termite guts exhibit this.

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Competition for Resources

This interaction involves microorganisms competing for limited nutrients and space. Those with quicker growth rates and better adaptability can outcompete others, often leading to the dominance of a particular microbe.

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Study Notes

Microbiology and Parasitology: History & Development

  • Microbiology is the study of nature, proceeding with questions about observations
  • Early Greek physicians and historians like Hippocrates, Thucydides, and Aristotle questioned disease links to environment and why some survived plagues.
  • Aristotle proposed spontaneous generation (life from non-living matter)
  • Antonie van Leeuwenhoek (1677) observed single-celled organisms (animalcules) using handcrafted microscopes.
  • Development was slow due to Leeuwenhoek's secrecy and scientists viewing microbes as insignificant in the 1700s.
  • Improvements in microscopes, new techniques, and experimental evidence propelled microbiology.
  • The term "microbe" was coined by Prof. Charles E. Sedillot (1804-1833).
  • Louis Pasteur demonstrated specific yeast and bacterial functions in fermentation and pasteurization methods between 1857-1860.
  • Martinus W. Beijerinck contributed to microbial ecology and isolated nitrogen-fixing microbes.
  • Microbes (prokaryotes) evolved around 3.6 billion years ago.

Microbiology and Parasitology: Key Contributors

  • Antonie Philips van Leeuwenhoek (1676): First to observe bacteria.

Microbiology and Parasitology: Animalcules

  • Term for microscopic organisms including bacteria, protozoans, and very small animals.

Microbiology and Parasitology: Edward Jenner

  • Developed the first vaccine for smallpox using cowpox virus, giving rise to the term "vaccine".

Microbiology and Parasitology: Louis Pasteur (1822-1895)

  • Considered the Father of Modern Microbiology and Bacteriology.
  • Proved principles of fermentation for food preservation.
  • Introduced sterilization techniques (steam sterilizer, hot air oven, autoclave).
  • Described pasteurization of milk.
  • Developed vaccines against anthrax, fowl cholera, and rabies.
  • Disproven spontaneous generation, proposing the Germ Theory of Disease.

Microbiology and Parasitology: Joseph Lister (1827-1912)

  • Considered the father of antiseptic surgery.
  • Reduced infections with surgical disinfectants, instrument sterilization, and wound cleaning.

Microbiology and Parasitology: Robert Koch (1843-1910)

  • Made significant contributions to microbiology.
  • Developed solid media for bacterial culture.
  • First to isolate bacteria in pure culture.
  • Discovered anthrax, tubercle, and cholera bacilli.
  • Introduced staining techniques using aniline dyes.
  • Developed Koch's postulates for proving pathogenicity.
  • Observed Koch's phenomenon (hypersensitivity reaction to tubercle bacilli).

Microbiology and Parasitology: Paul Ehrlich (1854-1915)

  • Considered the Father of chemotherapy.
  • Discovered acid-fast nature of tubercle bacillus.
  • Developed tissue and blood cell staining techniques.
  • Proposed toxin-antitoxin interaction (Ehrlich phenomenon).
  • Developed methods for standardizing toxins and antitoxins.
  • Provided the means for naming bacteria (E. coli).

Microbiology and Parasitology: Hans Christian Gram (1884)

  • Developed Gram staining method for visualizing and differentiating bacteria.

Microbiology and Parasitology: Charles Chamberland

  • Constructed a porcelain bacterial filter (1884), enabling virus discovery.

Microbiology and Parasitology: Other Important Figures

  • Ernst Ruska (electron microscope - 1931).
  • Alexander Fleming (penicillin - 1929).
  • Elie Melchnikoff (studied phagocytosis).
  • Waller Gilbert and Frederick Sanger (DNA sequencing - 1977)
  • Karry B Mullis (polymerase chain reaction - PCR - 1993).

Realm of Microbial Existence

  • Terrestrial Communities: microbes in soil, rainforests.
  • Aquatic Communities: microbes in oceans and other water bodies.
  • Extremophile Communities: microbes thriving in extreme environments (hot, acidic, salty etc).

Associations of Microorganisms with other Organisms

  • Neutralism: microbes coexist without affecting each other.
  • Positive: Mutualism (both benefit), Syntrophism (exchange nutrition), Proto-cooperation, Commensalism (one benefits, other unaffected).
  • Negative: Antagonism, Competition, Parasitism, Amensalism, Predation.

Microbes and Aseptic Techniques

  • Aseptic Techniques: procedures preventing contamination in microbiology.
  • Rules to follow during aseptic technique. Examples are outlined.

Microscopy and Techniques

General Principles:

  • Wavelength radiation: visible light and its wavelengths.
  • Magnification (using lenses that refract light)
  • Resolution: distinguish close objects (influenced by wavelength, numerical aperture).
  • Contrast: distinguish objects from background.
  • Common Laboratory Tools: homogenizer, hot plate, hot air oven, magnetic stirrer, colony counter, deep freezers.
  • Microscopy techniques: Wet mount, dry mount, smear, squash

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