Questions and Answers
What is the purpose of reducing media?
Aerobic respiration requires oxygen.
True
What is anaerobic respiration?
Respiration that does not require oxygen.
Define fermentation.
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Strict aerobe has growth at the ______ of the tube.
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Facultative anaerobe grows best where most oxygen is present, occurring throughout the ______.
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What color indicates the presence of oxygen in fluid thioglycollate tubes?
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Which organism is classified as an obligate anaerobe?
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Microaerophiles require high oxygen concentrations.
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What type of organism thrives in low oxygen conditions?
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What organism causes botulism?
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Match the antibiotic to the disease it treats:
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Study Notes
Reducing Media
- Designed to decrease oxygen levels for the cultivation of obligate anaerobes.
Aerobic Respiration
- A metabolic process that requires the presence of oxygen for energy production.
Anaerobic Respiration
- Metabolic process that occurs without oxygen, using other molecules as electron acceptors.
Fermentation
- A metabolic process that breaks down sugars into acids, gases, and alcohol in the absence of oxygen.
Fluid Thioglycollate Broth
- Supports the growth of various bacteria and assesses their oxygen requirements:
- Strict Aerobe: Growth at the top, where oxygen concentration is highest.
- Facultative Anaerobe: Grows best in high oxygen concentration, but also throughout the tube.
- Aerotolerant Anaerobe: Growth is uniform regardless of oxygen presence.
- Obligate Anaerobe: Growth exclusively at the bottom, where there is no oxygen.
Brewer Plates
- Used to differentiate organisms based on their growth in varying oxygen conditions:
- Strict Aerobe: Grows only on aerobic agar, turns purple.
- Facultative Anaerobe: Can grow in both oxygen-rich and oxygen-poor environments.
- Obligate Anaerobe: Grows only on anaerobic agar, appears blue without oxygen.
Fluid Thioglycollate Tubes Functions
- Tests bacterial aerotolerance utilizing the indicator dye resazurin, which turns red when oxygen is present.
- Growth patterns indicate oxygen requirements:
- Obligate aerobes flourish at the top.
- Obligate anaerobes thrive at the bottom.
- Microaerophiles establish growth in a thin layer just below the oxygen-rich region.
- Facultative and aerotolerant anaerobes largely occupy the middle zone.
Atmospheric Requirements
- Obligate Aerobe: Requires oxygen for survival.
- Obligate Anaerobe: Cannot survive in the presence of oxygen.
- Facultative Anaerobe: Can grow in both aerobic and anaerobic conditions.
- Microaerophile: Thrives in low oxygen levels, typically 2-10%.
Metabolic Functions Based on Atmospheric Conditions
- Obligate Anaerobe: Example - Clostridium species, observed growth at the bottom of the tube.
- Obligate Aerobe: Example - Bacillus subtilis, observed growth at the top of the tube.
Causative Anaerobic Organisms and Diseases
- Botulism: Caused by Clostridium botulinum.
- Gas Gangrene: Associated with Clostridium perfringens.
- Tetanus: Caused by Clostridium tetani.
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Description
Test your knowledge on aerotolerance in microbiology with these flashcards. You'll encounter key terms such as reducing media, aerobic respiration, and anaerobic respiration, which are essential for understanding microbial processes. Perfect for students studying microbiology or related fields.
