Microbiology Aerotolerance Flashcards
12 Questions
101 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of reducing media?

  • To promote fermentation
  • To inhibit growth of aerobic organisms
  • To increase oxygen levels
  • To enhance growth of obligate anaerobes (correct)
  • Aerobic respiration requires oxygen.

    True

    What is anaerobic respiration?

    Respiration that does not require oxygen.

    Define fermentation.

    <p>A metabolic process that catabolizes sugars to acids, gases, and alcohol.</p> Signup and view all the answers

    Strict aerobe has growth at the ______ of the tube.

    <p>top</p> Signup and view all the answers

    Facultative anaerobe grows best where most oxygen is present, occurring throughout the ______.

    <p>tube</p> Signup and view all the answers

    What color indicates the presence of oxygen in fluid thioglycollate tubes?

    <p>Red</p> Signup and view all the answers

    Which organism is classified as an obligate anaerobe?

    <p>Clostridium tetani</p> Signup and view all the answers

    Microaerophiles require high oxygen concentrations.

    <p>False</p> Signup and view all the answers

    What type of organism thrives in low oxygen conditions?

    <p>Microaerophile</p> Signup and view all the answers

    What organism causes botulism?

    <p>Clostridium botulinum</p> Signup and view all the answers

    Match the antibiotic to the disease it treats:

    <p>Botulism = Clostridium botulinum Gas gangrene = Clostridium perfringens Tetanus = Clostridium tetani</p> Signup and view all the answers

    Study Notes

    Reducing Media

    • Designed to decrease oxygen levels for the cultivation of obligate anaerobes.

    Aerobic Respiration

    • A metabolic process that requires the presence of oxygen for energy production.

    Anaerobic Respiration

    • Metabolic process that occurs without oxygen, using other molecules as electron acceptors.

    Fermentation

    • A metabolic process that breaks down sugars into acids, gases, and alcohol in the absence of oxygen.

    Fluid Thioglycollate Broth

    • Supports the growth of various bacteria and assesses their oxygen requirements:
      • Strict Aerobe: Growth at the top, where oxygen concentration is highest.
      • Facultative Anaerobe: Grows best in high oxygen concentration, but also throughout the tube.
      • Aerotolerant Anaerobe: Growth is uniform regardless of oxygen presence.
      • Obligate Anaerobe: Growth exclusively at the bottom, where there is no oxygen.

    Brewer Plates

    • Used to differentiate organisms based on their growth in varying oxygen conditions:
      • Strict Aerobe: Grows only on aerobic agar, turns purple.
      • Facultative Anaerobe: Can grow in both oxygen-rich and oxygen-poor environments.
      • Obligate Anaerobe: Grows only on anaerobic agar, appears blue without oxygen.

    Fluid Thioglycollate Tubes Functions

    • Tests bacterial aerotolerance utilizing the indicator dye resazurin, which turns red when oxygen is present.
    • Growth patterns indicate oxygen requirements:
      • Obligate aerobes flourish at the top.
      • Obligate anaerobes thrive at the bottom.
      • Microaerophiles establish growth in a thin layer just below the oxygen-rich region.
      • Facultative and aerotolerant anaerobes largely occupy the middle zone.

    Atmospheric Requirements

    • Obligate Aerobe: Requires oxygen for survival.
    • Obligate Anaerobe: Cannot survive in the presence of oxygen.
    • Facultative Anaerobe: Can grow in both aerobic and anaerobic conditions.
    • Microaerophile: Thrives in low oxygen levels, typically 2-10%.

    Metabolic Functions Based on Atmospheric Conditions

    • Obligate Anaerobe: Example - Clostridium species, observed growth at the bottom of the tube.
    • Obligate Aerobe: Example - Bacillus subtilis, observed growth at the top of the tube.

    Causative Anaerobic Organisms and Diseases

    • Botulism: Caused by Clostridium botulinum.
    • Gas Gangrene: Associated with Clostridium perfringens.
    • Tetanus: Caused by Clostridium tetani.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Description

    Test your knowledge on aerotolerance in microbiology with these flashcards. You'll encounter key terms such as reducing media, aerobic respiration, and anaerobic respiration, which are essential for understanding microbial processes. Perfect for students studying microbiology or related fields.

    More Like This

    Use Quizgecko on...
    Browser
    Browser