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Questions and Answers
Biosafety cabinets are designed to protect the sample from contamination.
False
Laminar hoods and biosafety cabinets serve the same purpose.
False
Biosafety cabinets can remove vapors and gases.
False
Biosafety cabinets are classified into two classes.
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Biosafety cabinets are used to sterilize biological materials.
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HEPA-filters remove particles smaller than 0.3 microns with an efficiency of 99.99%.
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The primary purpose of biosafety cabinets is to protect the sample.
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All biosafety cabinets are enclosed, ventilated laboratory workspaces.
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Class I Biosafety Cabinets provide protection to the product.
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Class I Biosafety Cabinets are typically used to enclose specific equipment like centrifuges.
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Class II Biosafety Cabinets do not provide protection to the environment.
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Class II Biosafety Cabinets have a fan mounted in the top of the cabinet that draws a curtain of unsterilized air over the workstation.
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Class I Biosafety Cabinets are always ducted to the building exhaust system.
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The air moving out of a Class I Biosafety Cabinet is not sterilized via the HEPA filters.
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Class II Biosafety Cabinets do not provide protection to the samples.
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Class I Biosafety Cabinets are suitable for procedures that do not generate aerosols.
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Class II BSCs are further divided into three types depending on the exhaust system and the mechanism of work.
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About 80% of all biosafety cabinets installed are Type A2 cabinets.
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Class III cabinets are leak-tight, totally enclosed but not ventilated cabinets.
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The cabinets are provided with metal gloves that are attached to the system to be used during operations in the cabinet.
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The exhaust air is treated with single HEPA filters or HEPA filters in combination with incineration.
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These cabinets can be used for only three Biosafety levels (1, 2, and 3).
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The filters have an unlimited lifespan.
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Cabinets need to be maintained on a irregular schedule.
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Study Notes
Microbial Safety Cabinet
- A biosafety cabinet (BSC) is an enclosed, ventilated laboratory workspace for safely working with contaminated materials or potentially contaminated pathogens, requiring a defined biosafety level.
Classification of Microbial Safety Cabinet
- Classified into three classes by the U.S. Centers for Disease Control and Prevention (CDC):
- Class I: provides protection to the environment and laboratory personnel, but not to the product.
- Class II: provides protection to the laboratory personnel, the environment, and the product.
- Class III: provides maximum protection to the operator, the environment, and the sample against high-risk group 4 pathogenic organisms.
Biosafety Cabinet Class I
- Provides protection to the environment and laboratory personnel, but not to the product.
- Used to enclose specific equipment or for procedures that might generate aerosols.
- Can be ducted (connected to the building exhaust system) or non-ducted (recirculating filtered exhaust back into the laboratory).
Biosafety Cabinet Class II
- Provides protection to both the laboratory personnel, the environment, and the product.
- Makeup air is HEPA-filtered.
- Divided into five types: Type A1, Type A2, Type B1, Type B2, and Type C1.
Biosafety Cabinet Class III
- Leak-tight, totally enclosed, and ventilated cabinets.
- All air that enters or leaves the facility passes through a HEPA filter.
- Provided with rubber gloves attached to the system and a transfer chamber for sterilization of materials.
Maintenance of Safety Cabinet
- Regular maintenance is necessary to verify airflow and filter capacities.
- Filters have a limited lifespan, and their replacement is necessary when they become loaded with particles and aerosols.
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Description
This quiz covers the fundamentals of Microbial Safety Cabinets, including their classification, principles, uses, types, and maintenance. It also touches on filtration and biological safety.
