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Questions and Answers
What is the primary role of sterols in the membranes of Mycoplasmataceae?
What is the primary role of sterols in the membranes of Mycoplasmataceae?
Which type of microorganism is capable of using either respiratory or fermentation processes based on oxygen availability?
Which type of microorganism is capable of using either respiratory or fermentation processes based on oxygen availability?
What is the function of superoxide dismutase in aerobic organisms?
What is the function of superoxide dismutase in aerobic organisms?
What do aerobic organisms typically produce that helps eliminate hydrogen peroxide?
What do aerobic organisms typically produce that helps eliminate hydrogen peroxide?
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Which of the following best describes anaerobic bacteria?
Which of the following best describes anaerobic bacteria?
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What occurs when superoxide interacts with oxidative enzymes?
What occurs when superoxide interacts with oxidative enzymes?
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What do aerotolerant organisms commonly rely on for enhanced growth?
What do aerotolerant organisms commonly rely on for enhanced growth?
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How do strict anaerobes interact with molecular oxygen?
How do strict anaerobes interact with molecular oxygen?
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What is the term used for organisms that prefer to grow under normal conditions of 37 °C, pH 7, and 0.9% saline?
What is the term used for organisms that prefer to grow under normal conditions of 37 °C, pH 7, and 0.9% saline?
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The presence of which type of compounds can lead to damage of DNA and other macromolecular structures in cells?
The presence of which type of compounds can lead to damage of DNA and other macromolecular structures in cells?
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What happens to the cell’s enzymes when exposed to suboptimal pH and temperature conditions?
What happens to the cell’s enzymes when exposed to suboptimal pH and temperature conditions?
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What role do signal transduction systems play in microbial stress responses?
What role do signal transduction systems play in microbial stress responses?
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How does the presence of unique membrane compositions impact an organism’s ability to survive in extreme environments?
How does the presence of unique membrane compositions impact an organism’s ability to survive in extreme environments?
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Why might microorganisms die when exposed to suboptimal environments?
Why might microorganisms die when exposed to suboptimal environments?
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What is one function of the proteins increased in expression during a microbial stress response?
What is one function of the proteins increased in expression during a microbial stress response?
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Why is it important for students to develop an integrated view of microbial physiology?
Why is it important for students to develop an integrated view of microbial physiology?
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Study Notes
Sterols in Microbial Membranes
- Most bacterial membranes lack sterols but some Mycoplasmataceae members require them for growth.
- Mycoplasma require sterols for growth, while Acholeplasma do not but produce terpenoid compounds that function similarly.
- Fungi (yeasts and molds) contain sterols, generally capable of synthesizing them.
Oxygen Requirements for Microbial Growth
- Aerobes need oxygen for energy metabolism, while anaerobes cannot utilize it.
- Facultative organisms can use either respiration or fermentation depending on oxygen availability.
- Aerobic organisms possess cytochromes and cytochrome oxidase, essential for oxidative phosphorylation.
- Oxygen acts as the terminal electron acceptor, leading to water production during respiration.
- Superoxide (O2-), hydroxyl radicals (OH-), and hydrogen peroxide (H2O2) are toxic by-products of oxygen metabolism.
- Superoxide dismutase eliminates superoxide, found in aerobes, aerotolerant organisms, but not strict anaerobes.
- Many, but not all, aerobes produce catalase to eliminate hydrogen peroxide.
- Aerotolerant organisms generally lack catalase, often benefiting from media containing catalase or peroxidase activity.
- Anaerobes do not interact with oxygen, tolerating it due to lack of superoxide or peroxide generation.
Carbon Dioxide Dependence
- Many organisms rely on carbon dioxide fixation.
- Some organisms thrive better in atmospheres with increased carbon dioxide.
Microbial Adaptation to Extreme Environments
- Organisms inhabiting extreme environments often possess unique membrane compositions and specialized enzymes adapted to temperature or pH optima.
Microbial Stress Responses
- Normalophiles thrive at 37°C, pH 7, and 0.9% salinity, deviating conditions impact enzyme activity and cell viability.
- Stress response systems detect environmental deterioration, such as acidification, triggering signal transduction pathways.
- These pathways activate transcription/translation of protective proteins, safeguarding the cell from stress.
- The genetic regulatory systems and protection strategies are further discussed in Chapters 5 and 18.
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Description
Explore the critical roles of sterols in microbial membranes and the varying oxygen requirements for microbial growth. Understand how different organisms utilize oxygen and the implications of by-products produced during respiration. This quiz covers essential concepts related to microbial physiology.