Microbial Growth: Observation and Requirements

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26 Questions

What is the ideal osmotic pressure condition for microbial survival?

Isotonic environment

Which of the following is an example of a psychrophile?

Organisms that prefer cold temperatures

What is the main reason microbes need carbon?

To produce energy

Which of the following statements about temperature preferences of microbes is correct?

Thermophiles prefer high temperatures

What is the effect of unequal osmotic pressure inside and outside a microbial cell?

Cell shrinkage leading to cell death

Which microbial category thrives in high salt environments by balancing osmotic pressure?

Halophiles

What is the primary source of carbon for microbes that are autotrophs?

$CO_2$

What is the significance of macronutrients for microbial growth?

They act as energy sources for metabolism

Which environmental factor plays a crucial role in determining the growth characteristics of bacteria and molds?

Temperature

What is the term for introducing microbes into a medium?

Inoculum

Which type of media is known for having a varying chemical composition from batch to batch?

Complex media

What is the main purpose of selective media?

Suppress unwanted microbes and encourage growth of desired microbes

Which type of media is considered non-selective and stimulates the growth of various bacterial species?

Enrichment media

What is the process called when bacteria increase in number through cell division?

Binary fission

Which method of measuring microbial growth involves counting cells directly?

Direct microscopic count

What is the term for a population of cells originating from a single cell or group of attached cells?

Colony

What type of media allows differentiation between bacteria within the same group?

Differential media

What is the term used to describe a population of cells from a single cell or group of attached cells?

Colony

Which organic compounds contain nitrogen?

Proteins

Which type of media stimulates the growth of many different types of bacterial species and often exists as liquid?

Enrichment media

Which organic compound contains phosphorus?

Nucleic acids

What are micronutrients also known as?

Trace elements

Which chemicals are usually function as inorganic enzyme cofactors?

Iron, copper, zinc

What is the function of organic growth factors in microbes?

Energy production

What type of microbes grow in liquid (broth) media?

Facultative anaerobes

What are solid media for microbial growth made of?

Agar

Learn about how microbial growth is determined in the lab through observation of culture mediums like Nutrient Agar and Nutrient Broth. Explore the requirements for microbial growth such as temperature, pH, osmotic pressure, and chemical nutrients.

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