Microbial Growth, Biofilms
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Questions and Answers

What is the primary outcome of microbial growth?

  • Formation of biofilms on various surfaces.
  • Cell division leading to a larger population. (correct)
  • Adaptation to diverse environmental conditions.
  • Increase in individual cell size without division.

Why are biofilms a concern of persistent infections in the healthcare setting?

  • Bacteria within biofilms are highly protected and difficult to eradicate. (correct)
  • They allow for easy access of antibiotics to all bacterial cells.
  • They prevent bacteria from adhering to surfaces..
  • They consist of a single layer of bacteria that is easily destroyed.

How does E. coli adapt during urinary tract infections (UTIs)?

  • It maintains its motile bacillus form without any change.
  • It forms a rigid biofilm structure within the bladder.
  • It transforms from a nonmotile filamentous shape to a motile bacillus form.
  • It converts from a motile bacillus to a nonmotile filamentous shape. (correct)

A research lab is trying to replicate a natural microbial community in vitro. Considering that only a small fraction of environmental bacteria can be cultured in labs, which approach would MOST enhance the diversity of the cultured community?

<p>Employing a co-culture system that mimics the complex nutrient interactions of natural environments. (A)</p> Signup and view all the answers

In a long-term indwelling catheter, why is the urine flow ineffective at removing established biofilms?

<p>The biofilm is established in a way that urine cannot flush out the adhered bacteria. (C)</p> Signup and view all the answers

Flashcards

Microbial Growth

Cell division leading to an increased population of cells.

Pure Culture

A single species of microbe grown in a lab setting.

Biofilms

Complex communities of microorganisms attached to a surface, encased in a matrix.

Biofilm Formation

Planktonic bacteria attach to a surface to start these

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Bacteria in Biofilms

Methicillin-resistant Staphylococcus Aureus, Vancomycin-resistant Enterococcus, Clostridium difficile, and Pseudomonas Aeruginosa

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Study Notes

  • When nutritional needs are satisfied, a microbe will grow in size and divide.
  • Microbial growth happens when cell division creates new daughter cells, which increases the cell population.
  • In the lab, microbial growth is grown as pure single-species cultures.
  • Only about 1% of the total bacterial species can be grown in a lab environment.
  • In nature, microbes grow in mixtures and in diverse environments
  • Environmental factors affect a bacteria's life, metabolism, and structure.
  • E.coli can change from a motile bacillus shape to a non-motile filamentous shape during UTIs.
  • Biofilms are the best way to see how microbes grow and live in their natural environment to survive.
  • In healthcare settings, biofilms are hard to treat, and cause persistent infections.
  • Plaque on the surface of a biofilm can be destroyed, but the cells deeper down are highly protected.
  • Biofilms form when planktonic bacteria stick to a surface.
  • Biofilm-forming bacteria that complicate treatment include:
    • Methicillin-resistant Staphylococcus Aureus
    • Vancomycin-resistant Enterococcus
    • Clostridium difficile
    • Pseudomonas Aeruginosa
  • Biofilms are commonly found in catheters and heart valves.
  • In a catheter, biofilm can break off in the urethra and stick to the bladder.
  • Urine flow is unable to flush out biofilm in a catheter.

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Description

Microbial growth occurs when cell division makes new daughter cells, expanding the cell population. Environmental factors impact a bacterium's life, metabolism, and structure. Biofilms show microbial growth in natural settings and are difficult to treat in healthcare, causing persistent infections.

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