Podcast
Questions and Answers
What is the primary outcome of microbial growth?
What is the primary outcome of microbial growth?
- Formation of biofilms on various surfaces.
- Cell division leading to a larger population. (correct)
- Adaptation to diverse environmental conditions.
- Increase in individual cell size without division.
Why are biofilms a concern of persistent infections in the healthcare setting?
Why are biofilms a concern of persistent infections in the healthcare setting?
- Bacteria within biofilms are highly protected and difficult to eradicate. (correct)
- They allow for easy access of antibiotics to all bacterial cells.
- They prevent bacteria from adhering to surfaces..
- They consist of a single layer of bacteria that is easily destroyed.
How does E. coli adapt during urinary tract infections (UTIs)?
How does E. coli adapt during urinary tract infections (UTIs)?
- It maintains its motile bacillus form without any change.
- It forms a rigid biofilm structure within the bladder.
- It transforms from a nonmotile filamentous shape to a motile bacillus form.
- It converts from a motile bacillus to a nonmotile filamentous shape. (correct)
A research lab is trying to replicate a natural microbial community in vitro. Considering that only a small fraction of environmental bacteria can be cultured in labs, which approach would MOST enhance the diversity of the cultured community?
A research lab is trying to replicate a natural microbial community in vitro. Considering that only a small fraction of environmental bacteria can be cultured in labs, which approach would MOST enhance the diversity of the cultured community?
In a long-term indwelling catheter, why is the urine flow ineffective at removing established biofilms?
In a long-term indwelling catheter, why is the urine flow ineffective at removing established biofilms?
Flashcards
Microbial Growth
Microbial Growth
Cell division leading to an increased population of cells.
Pure Culture
Pure Culture
A single species of microbe grown in a lab setting.
Biofilms
Biofilms
Complex communities of microorganisms attached to a surface, encased in a matrix.
Biofilm Formation
Biofilm Formation
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Bacteria in Biofilms
Bacteria in Biofilms
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Study Notes
- When nutritional needs are satisfied, a microbe will grow in size and divide.
- Microbial growth happens when cell division creates new daughter cells, which increases the cell population.
- In the lab, microbial growth is grown as pure single-species cultures.
- Only about 1% of the total bacterial species can be grown in a lab environment.
- In nature, microbes grow in mixtures and in diverse environments
- Environmental factors affect a bacteria's life, metabolism, and structure.
- E.coli can change from a motile bacillus shape to a non-motile filamentous shape during UTIs.
- Biofilms are the best way to see how microbes grow and live in their natural environment to survive.
- In healthcare settings, biofilms are hard to treat, and cause persistent infections.
- Plaque on the surface of a biofilm can be destroyed, but the cells deeper down are highly protected.
- Biofilms form when planktonic bacteria stick to a surface.
- Biofilm-forming bacteria that complicate treatment include:
- Methicillin-resistant Staphylococcus Aureus
- Vancomycin-resistant Enterococcus
- Clostridium difficile
- Pseudomonas Aeruginosa
- Biofilms are commonly found in catheters and heart valves.
- In a catheter, biofilm can break off in the urethra and stick to the bladder.
- Urine flow is unable to flush out biofilm in a catheter.
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