16 Questions
Match the following organic compounds with their components:
Proteins = Nitrogen and sulfur Nucleic acids = Nitrogen and phosphorus Sulfur-containing amino acids = Sulfur Nucleotides = Phosphorus
Match the following terms with their descriptions:
Micronutrients = Chemicals required by microbes in small quantities Inorganic enzyme cofactors = Iron, copper, zinc Organic growth factors = Vitamins A & D Culture Media = Preparation for microbial growth
Match the following oxygen requirements with their descriptions:
Obligate aerobes = Require oxygen to survive Obligate anaerobes = Cannot survive in the presence of oxygen Facultative anaerobes = Can survive with or without oxygen Microaerophiles = Require reduced levels of oxygen
Match the following media types with their descriptions:
Solid (agar) media = Used for microbial growth with visible colonies Liquid (broth) media = Used for microbial growth without solidification Nutrient agar = Visible colonies on solid media Nutrient broth = Growth in liquid media without solidification
Match the following terms with their functions in microbial growth:
Proteins = Energy source for heterotrophs Nucleic acids = Contain genetic information for cells Iron, copper, zinc = Act as enzyme cofactors Vitamins A & D = Organic growth factors for microbes
Match the following temperature descriptions with their correct terms:
Minimum growth temperature = The lowest temperature at which a microbe can grow Maximum growth temperature = The highest temperature at which a microbe can grow Optimum growth temperature = The temperature at which a microbe grows best Temperature Preferences of Some Microbes = Categories like Psychrophiles, Mesophiles, Thermophiles
Match the following pH descriptions with their correct terms:
pH of Environment = Measure of acidity or alkalinity where microbes grow Bacteria = Microbes that prefer neutral pH levels Molds and yeasts = Microbes that thrive in acidic environments Acidophiles = Microbes that can survive in highly acidic conditions
Match the following osmotic pressure effects with their correct outcomes:
If the osmotic pressure is not equal inside and outside the cell = Cell will shrink or swell and eventually die Halophiles = Microbes that can survive in high salt environments by balancing osmotic pressure Cells in an isotonic solution = Ideal condition for cell survival due to balanced osmotic pressure Cells capable of producing positively-charged solutes inside = Able to balance out external osmotic pressure and survive
Match the following nutrient descriptions with their correct terms:
Macronutrients = Chemicals required by microbes in large quantities Micronutrients = Chemicals required by microbes in small amounts Carbon and Nitrogen = Important elements used by microbes for energy production and metabolism Phosphorus = One of the essential macronutrients needed by microbes
Match the following carbon descriptions with their correct terms:
Why do microbes need carbon? = To produce energy Where does the carbon come from? = CO2 for autotrophs is a source of carbon for energy production Nitrogen = An important element needed by microbes along with carbon for various functions Chemical elements used by microbes = Includes Carbon, Nitrogen, Sulfur, Phosphorus as essential nutrients
Match the following terms with their definitions:
Inoculum = Introduction of microbes into a medium Culture = Microbes growing in or on a culture medium Pure culture = Contains only one species seen on solid agar plate Colony = Population of cells from a single cell or from a group of attached cells
Match the following types of culture media with their descriptions:
Chemically Defined Media = Exact chemical composition is known Complex Media = Chemical composition varies from batch to batch Reducing Media = All available O2 removed for growth of anaerobic bacteria Selective Media = Suppress unwanted microbes and encourage growth of desired microbes
Match the following terms related to bacterial growth with their explanations:
Bacterial Division = Increase in number of cells, not cell size Generation Time = Time required for a cell to divide (to double) Direct Measurements = Counting methods like plate count and direct microscopic count Indirect Measurements = Methods like turbidity and metabolic activity, not counting
Match the following terms with their roles in microbial growth:
Streak Plate Method = Used to isolate pure bacterial cultures Plaque-forming unit (PFU) = Formed in viral growth to represent viral plaques Enrichment Media = Considered a non-selective medium stimulating growth of many different bacterial species Plate Counts = Follows steps like plate count, filtration method, and direct microscopic count
Match the following media types with their characteristics:
Differential Media = Allow differentiation between bacteria within the same group Enrichment Media = Stimulate growth of many different types of bacterial species Complex Media = Chemical composition varies from batch to batch Selective Media = Suppress unwanted microbes and encourage growth of desired microbes
Match the following terms with their representations in microbial growth:
Colony-forming unit (CFU) = A colony representing a population of cells from a single cell or from a group of attached cells Viral Plaque = Formed in viral growth to represent viral plaques Generation Time = Time required for a cell to divide (to double) Bacterial Growth Curve = A representation showing the stages of bacterial growth
Test your knowledge on microbial growth by learning how it is determined in the lab, observing culture mediums, bacterial growth, colony formation, and the physical and chemical requirements for microbial growth such as temperature, pH, osmotic pressure, and nutrient requirements.
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