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Questions and Answers
What is the highest known temperature at which a microorganism can survive?
What is the highest known temperature at which a microorganism can survive?
Which of the following is NOT an example of an extreme environment discussed in the lecture?
Which of the following is NOT an example of an extreme environment discussed in the lecture?
What type of organism can be found 3.2 km underground?
What type of organism can be found 3.2 km underground?
Besides cellular adaptations, what other type of relationships will be discussed in the second part of the lecture?
Besides cellular adaptations, what other type of relationships will be discussed in the second part of the lecture?
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What is the primary focus of the first part of the lecture?
What is the primary focus of the first part of the lecture?
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Which of the following marine organisms are mentioned as being amongst the first to colonise an area?
Which of the following marine organisms are mentioned as being amongst the first to colonise an area?
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What role does hydrogen sulfide play in the life cycle of Beggiatoa?
What role does hydrogen sulfide play in the life cycle of Beggiatoa?
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What is the main function of the intracellular sulfur granules found in Beggiatoa?
What is the main function of the intracellular sulfur granules found in Beggiatoa?
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What is the range of size for Beggiatoa filaments?
What is the range of size for Beggiatoa filaments?
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Where do Beggiatoa organisms typically reside in marine sediments?
Where do Beggiatoa organisms typically reside in marine sediments?
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What is the eventual fate of the elemental sulfur stored in Beggiatoa?
What is the eventual fate of the elemental sulfur stored in Beggiatoa?
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What gives Beggiatoa its opaque appearance?
What gives Beggiatoa its opaque appearance?
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Which of these terms best describes Beggiatoa?
Which of these terms best describes Beggiatoa?
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Which of the following best describes the role of Thiobacillus ferroxidans in acid mine drainage?
Which of the following best describes the role of Thiobacillus ferroxidans in acid mine drainage?
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What is the primary role of Fe2+ in acid-tolerant microbes in the context of pH regulation?
What is the primary role of Fe2+ in acid-tolerant microbes in the context of pH regulation?
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In the oxidation of FeS2, what role does atmospheric oxygen play?
In the oxidation of FeS2, what role does atmospheric oxygen play?
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How does the interaction of acid effluent with mineral compounds affect environmental organisms as described?
How does the interaction of acid effluent with mineral compounds affect environmental organisms as described?
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What happens to the concentration of Fe3+ and Fe2+ ions as the metabolic reactions continue in an acid environment?
What happens to the concentration of Fe3+ and Fe2+ ions as the metabolic reactions continue in an acid environment?
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Why is iron considered an energetically unfavorable electron donor for ATP production compared to NAD?
Why is iron considered an energetically unfavorable electron donor for ATP production compared to NAD?
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What is meant by the 'buffering capacity' of a watershed's soil, as mentioned in the text?
What is meant by the 'buffering capacity' of a watershed's soil, as mentioned in the text?
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According to the content, what is the typical pH range of most lakes and streams?
According to the content, what is the typical pH range of most lakes and streams?
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What role does organic matter play in the context of iron in hydrothermal plumes?
What role does organic matter play in the context of iron in hydrothermal plumes?
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What are the main components of pyrite nanoparticles found in hydrothermal fluids?
What are the main components of pyrite nanoparticles found in hydrothermal fluids?
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How do Arcobacter species adapt to high-fluid-flow environments near hydrothermal vents?
How do Arcobacter species adapt to high-fluid-flow environments near hydrothermal vents?
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What is the primary food source for Pompeii worms?
What is the primary food source for Pompeii worms?
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What is a unique characteristic of Pompeii worms in terms of their heat tolerance?
What is a unique characteristic of Pompeii worms in terms of their heat tolerance?
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Which of the following best describes how iron is transformed near hydrothermal vents, as it precipitates?
Which of the following best describes how iron is transformed near hydrothermal vents, as it precipitates?
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What happens to methane as it diffuses up through sediments near hydrothermal vents?
What happens to methane as it diffuses up through sediments near hydrothermal vents?
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What does the process of fixing CO2 into cell carbon near hydrothermal vents involve?
What does the process of fixing CO2 into cell carbon near hydrothermal vents involve?
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Which bacterial genera are identified as being involved in the conversion of methane and sulfate to bicarbonate and sulfide?
Which bacterial genera are identified as being involved in the conversion of methane and sulfate to bicarbonate and sulfide?
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What is the role of archaeal species in the described metabolic partnership with bacteria?
What is the role of archaeal species in the described metabolic partnership with bacteria?
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In the anaerobic oxidation of methane, what substances are produced by the bacteria?
In the anaerobic oxidation of methane, what substances are produced by the bacteria?
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In the diagram showing the anaerobic oxidation of methane in marine sediments, what is the main substance that is depicted moving upwards from the anaerobic sediment to the aerobic sediment?
In the diagram showing the anaerobic oxidation of methane in marine sediments, what is the main substance that is depicted moving upwards from the anaerobic sediment to the aerobic sediment?
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What function do the root-like structures of Riftia sp. serve?
What function do the root-like structures of Riftia sp. serve?
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Where is the sulfide absorbed by L. luymesi transported to after being absorbed by its root-like structures?
Where is the sulfide absorbed by L. luymesi transported to after being absorbed by its root-like structures?
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What is the primary source of nutrition for adult worms in the described symbiotic relationship?
What is the primary source of nutrition for adult worms in the described symbiotic relationship?
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What is the primary role of the vestimentiferan worm's root-like structures?
What is the primary role of the vestimentiferan worm's root-like structures?
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Based on the text, which statement is true regarding the locations of the different types of sediments?
Based on the text, which statement is true regarding the locations of the different types of sediments?
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Which metabolic process do the bacteria use within the worm to generate energy?
Which metabolic process do the bacteria use within the worm to generate energy?
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What term describes organisms adapted to live in very cold environments?
What term describes organisms adapted to live in very cold environments?
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What is the primary mechanism by which microbes in icy/cold environments maintain their membrane fluidity?
What is the primary mechanism by which microbes in icy/cold environments maintain their membrane fluidity?
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What is the substance synthesized by algal and bacterial cells in ice matrices that creates a protective shell?
What is the substance synthesized by algal and bacterial cells in ice matrices that creates a protective shell?
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What cellular structure is formed by a combination of living cells and their own produced extracellular matrix in cold environments?
What cellular structure is formed by a combination of living cells and their own produced extracellular matrix in cold environments?
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What does the RNA analysis of cold water microbes suggest about their primary mode of respiration?
What does the RNA analysis of cold water microbes suggest about their primary mode of respiration?
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Besides photosynthesis, what additional metabolic process is indicated to occur in a limited way in cold water microbes?
Besides photosynthesis, what additional metabolic process is indicated to occur in a limited way in cold water microbes?
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Flashcards
Extreme environments
Extreme environments
Habitats with conditions that are outside the range of what is considered 'normal' for most life forms. These conditions can be extremely hot, cold, acidic, saline, or have other extreme features.
Extremophiles
Extremophiles
Organisms that live in extreme environments. These organisms are very specialized to survive in their unique habitats.
Acidic environments
Acidic environments
Environments that are very acidic, with a pH lower than that of most natural environments. Many bacteria and archaea are well-adapted to survive in acidic environments.
High saline environments
High saline environments
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Microbial adaptations
Microbial adaptations
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Pyrite oxidation
Pyrite oxidation
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Acid mine drainage
Acid mine drainage
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Acid tolerance
Acid tolerance
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Abiogenic oxidation of Fe2+
Abiogenic oxidation of Fe2+
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Iron as an electron donor
Iron as an electron donor
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Proton motive force (PMF)
Proton motive force (PMF)
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Buffering capacity
Buffering capacity
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Acidification of lakes and streams
Acidification of lakes and streams
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Archaea-Bacterial Symbiosis
Archaea-Bacterial Symbiosis
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Anaerobic Oxidation of Methane
Anaerobic Oxidation of Methane
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Vestimentiferan Worm
Vestimentiferan Worm
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Trophosome
Trophosome
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Root-like Structures
Root-like Structures
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Sulfide (H2S)
Sulfide (H2S)
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Sulfate-reducing bacteria (Desulfovibrio and Desulfococcus)
Sulfate-reducing bacteria (Desulfovibrio and Desulfococcus)
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Methane (CH4)
Methane (CH4)
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Beggiatoa
Beggiatoa
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Lithoautotrophy
Lithoautotrophy
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Hydrogen sulfide (H2S)
Hydrogen sulfide (H2S)
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Sulfur
Sulfur
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Sulfur oxidation
Sulfur oxidation
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Sulfate (SO42-)
Sulfate (SO42-)
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Sulfur conversion
Sulfur conversion
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Sulfur cycle
Sulfur cycle
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Symbiotic bacteria in vestimentiferan worms
Symbiotic bacteria in vestimentiferan worms
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Psychrophiles
Psychrophiles
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Biofilm
Biofilm
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Microorganisms living in ice
Microorganisms living in ice
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RNA analysis
RNA analysis
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Dissimilatory nitrate/nitrite reduction
Dissimilatory nitrate/nitrite reduction
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Iron in marine environments
Iron in marine environments
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Iron transport in hydrothermal plumes
Iron transport in hydrothermal plumes
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Pyrite nanoparticles in hydrothermal plumes
Pyrite nanoparticles in hydrothermal plumes
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Carbon fixation in hydrothermal vent ecosystems
Carbon fixation in hydrothermal vent ecosystems
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Arcobacter adaptation to hydrothermal vent environments
Arcobacter adaptation to hydrothermal vent environments
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Pompeii worm feeding strategy
Pompeii worm feeding strategy
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Pompeii worm heat tolerance
Pompeii worm heat tolerance
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Iron removal from the water column
Iron removal from the water column
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Study Notes
Microbial Adaptation to Extreme Environments
- Learning Outcomes: Students should be able to describe microbial adaptations to various environmental niches. They should also be able to provide examples of species for different environments and understand the relationships between organisms in terms of predation and metabolic adaptations.
Structure of Today's Lecture
- The lecture is divided into two parts:
- Part 1: Focuses on cellular adaptations to extreme habitats.
- Part 2: Examines relationships between organisms in extreme environments.
Fun Facts
-
Hottest Environment: Juan de Fuca ridge, 121°C, Geogemma barossii.
-
Deepest Organisms: Crypto-endoliths at 3.2 km underground.
What are "Extreme" Environments?
- Examples: Acidic environments and high saline environments.
Anthropogenic Acid Tolerance
- Pyrite (FeS₂): Oxidized by Thiobacillus ferroxidans to H₂SO₄ in acid mine drainage.
- pH: Acid mine drainage can reach ~pH3, creating environments unsuitable for most other life forms.
- Oxidation Process: The oxidation of Fe²⁺.
- Metabolic Reactions: Lead to a decrease in pH, with Fe³⁺ predominating as an electron acceptor.
- Acidophiles: Oxidize Fe²⁺, which serves as an electron acceptor.
- Mineral Compounds: Acid effluent interacts with mineral compounds, reducing the availability of trace elements to organisms (e.g., aluminum).
Iron as an Electron Donor
- Energetically Unfavorable: Iron is an energetically unfavorable electron donor for ATP production, compared to NAD.
- Proton Motive Force (PMF): Acid-tolerant microbes use PMF to allow H⁺ entry, which must be neutralized to prevent cytoplasmic pH drops.
- Iron's Function: Iron neutralizes the H⁺, preventing cell death
Anthropogenic Acid Tolerance (continued)
- Buffering Capacity: Water and surrounding soil can neutralize acid rain, but in areas with low buffering capacity, acid rain releases toxic aluminum into lakes and streams.
- Effect on Life: Aluminum is harmful to aquatic organisms.
The Effect of Acidity on Selected Life Forms
- A table displaying the impacts of acidity on different species: Trout, Bass, Perch, Frogs, Salamanders, Clams, Crayfish, Snails, and Mayflies, showing the correlation between pH and species survival.
Case Study: Scottish Lakes
- Industrial Pollution & Acid Rain: Pollution and acid rain negatively impacted lakes and rivers in Northern Europe and America.
- Fish Stock Collapse: Fish stocks collapsed, and ecosystems suffered damage.
- EU Sulphur Emission Reductions: EU actions to limit sulphur emissions have resulted in improvement of the situation.
Case Study: Scottish Lakes (continued)
- Food Web Changes: Monitoring indicates changes in food webs, but not necessarily in a predictable way. The number of species in affected lakes is still low, and oxygen production by algae and trout health are also negatively affected.
Adaptation to Low Temperatures (Psychrophiles):
- Enzymes: Psychrophiles produce enzymes with lower temperature optima and often denature at room temperatures.
- Membrane Composition: Psychrophiles possess higher unsaturated fatty acids & short-chain membrane lipids, which keeps the membranes fluid at lower temperatures.
- Example Species: Desulfofaba gelida (bottom) and Psychromonas ingrahamii (top)
- Optimal Temperatures: A table showing minimum growth and maximum growth temperatures for various eubacterial psychrophile species.
- Vibrio Marinus: Microscopic image showing (CW), (PM), and (PR) of the species.
Membrane Fluidity
- High-temperature adapted membranes are not functional in low temperatures.
- Different membrane structure in Archaea (ether linkages) and Eubacteria (ester links).
- Hydrophobic bonds and bonding forces plus hydrocarbon branching make Archaea membranes more heat tolerant.
Survival & Death at Extreme Temperatures
- Microbial activity decreases below the optimal temperature (basis for food refrigeration).
- In natural soil and water, microbes generally present slower metabolic activity during winter due to decreasing ambient temperatures.
- Some bacteria produce endospores; fungi, sclerotia
Effect of Temperature on Microbial Activity
- Higher temperatures (that don't kill cells) increase metabolic activities, such as oxygen consumption.
- Increased respiration correlates with increased enzyme activity, until protein denaturation occurs.
Q₁₀ Values (Relative)
- Q₁₀: A measure of how enzyme activity changes with a 10°C temperature increase.
- Values for selected enzymes are provided
High Salt Environments (Halophiles):
- Halophiles thrive in environments with high levels of dissolved ions, leading to reduced water availability for respiration.
Water Availability
- In hypertonic environments, non-halophiles dehydrate.
- High salt concentrations denature proteins.
- Halophiles exclude sodium ions to prevent toxicity.
- Example: Dunaliella (alga) builds up glycerol, and Halobacterium accumulates potassium ions.
Halophile Adaptations
- Example Genus: Halobacterium
- Metabolic Requirements: Require at least 0.3 M NaCl for growth.
- Bacteriorhodopsin: Some strains possess bacteriorhodopsin in cell membranes, acting as light-driven proton pumps that generate electrical potential to drive ATP synthesis through PMF.
Halophiles and Light Utilisation
- Halobacteria use light to generate PMF for:
- Motility
- ATP production
- Sodium pump operation
- Uptake of organic nutrients
Bacterial Rhodopsin
- Structure: A 26 kDa pigmented protein.
- Function: Acts as a H⁺ pump, seen as purple patches on the cell membrane.
Summary Points
- Adaptation Strategies: Organisms adapt to survive by :
- preventing entry of toxic compounds
- utilizing environmental resources for benefit
- Examples: Acidophiles use H⁺ ions and halophiles use light energy.
Hydrothermal Vents
- Formation: Cold seawater is heated at mid-ocean ridges, rising as hot water with high mineral content.
- Temperature: Water temperature can exceed 340°C but doesn't boil due to extreme pressure.
Life Away from the Sun
- Chemosynthetic Bacteria: Bacteria use sulfur compounds to produce organic material via chemosynthesis, forming the base of vent food webs.
- Essential for Food Web: Vent animals rely on chemosynthetic bacteria for food.
Lithoautotrophs
- Beggiatoa: Genus in sediments oxidized reduced hydrogen sulphide, creating elemental sulfur granules, affecting appearance.
Biochemistry & Metabolism
- Beggiatoa can perform biogenic oxidation. This process converts hydrogen sulphide to elemental sulphur stored as intracellular sulfur globules. Sulphur granules can further convert to sulphate through metabolism. Bacteria can also utilise organic compounds if available.
What about Trace Elements (Iron)?
- Limiting Micronutrient: Iron is a crucial micronutrient.
- Oxidization/Precipitation: Vent-derived iron was previously thought to quickly oxidize and precipitate around vents.
- Organic Matter Stabilization: However, organic matter can bind to the dissolved/particulate iron, leading to greater dispersion into the ocean.
- Pyrite Nanoparticles: Iron and sulphur pyrites form particles up to 10% of the filterable iron in fluids.
Other Elements
- Elements such as copper, manganese, zinc, sulphur, and silicone are found in hydrothermal vent ecosystems.
- Carbon: CO₂ is fixed using energy from the oxidation of sulphides
Bacteria as Food
- Arcobacter Species: These bacteria live in sulphide-rich high flow environments, creating rigid filamentous mats that resist turbulence.
- Alvinella Worms (Pompeii Worms): Feed on microbial mats, utilizing bacterial products for survival.
Other Animal-Microbe Interactions (continued)
- Pompeii Worms: These worms are significantly heat-tolerant, living near vents and able to withstand 105°C water.
Methane Cycling and Symbioses
- Methane Utilization: Methane diffuses up in sediments, metabolized by sulfur metabolisers before reaching oxic zones.
- Bacterial Genuses: Desulfovibrio and Desulfococcus are identified bacteria.
- Metabolic Partnerships: Methane and sulfate produced by Archaea are converted to bicarbonate and sulfide by Eubacteria.
Archaea-Bacterial Symbioses
- Archaea and Bacterial Symbiotic Relationship
Anaerobic Oxidation of Methane in Marine Sediments
- Methane's movement through, and conversion in, sediments.
- Archaea and Eubacteria in partnership.
Vestimentiferan Worms
- Riftia sp.: Root-like structures extend into the sediment to absorb sulphide, and this compound is transported to the trophosome.
Interactions with Worms
- Seawater Conditions: Various SO₄²⁻ concentration and pH levels in seawater, body fluids, and sediment.
- Microbe Chemical Reactions: Shows a chemical reaction of CH₄+H⁺+SO₄²⁻ to H₂S+H₂O+HCO₃− by microbes.
Worm-Bacterial Symbioses
- Nutritional Dependence: Adult worms nutritionally depend on their bacterial symbionts.
- Reduced Inorganic Sulphur: Oxidization of these compounds produces energy using reducing power for carbon fixation.
Life in the Freezer (Subzero Waters)
- Micro-organisms inhabit ice crystals and sub-zero waters.
Cellular Adaptations to Living in Ice or Cold Water Environments
- Psychrophiles: Organisms living in ice or cold water environments.
- Low Nutrient Conditions: Arctic and Antarctic waters are low in nutrients.
- Adaptations Required: Microbes adapt to survive in low nutrient environments.
- Autotrophic Microorganisms: RNA analysis for photosynthesis-related genes reveals the presence of autotrophs.
- Aerobes: Aerobic microbes are common in cold marine environments. Growth in anaerobic conditions is possible but not widespread.
- Cryoprotectants and Membrane Fluidity: Metabolic adaptations in cold environments include producing cryoprotectants and maintaining membrane fluidity, along with unsaturated fatty acids.
Cellular Adaptations to the Cold
- Exopolysaccharide Production: The combination of life cells within extracellular matrix formation, also known as biofilm, reduces the effect of extreme cold.
Psychroflexus torquis
- Sea ice salinity conditions and their influence on the species' growth.
- Optimal salt concentration for growth, fluctuating salt levels
- Proteorhodopsins possibly play a role in homeostasis.
Bacteria Living Within the Ice
- Bacteria in solid ice maintain active metabolisms.
- Freezing principles have been used for bacterial preservation.
Sulphuric Acid Drips
- Sulphuric acid drips create pools of acidic water.
Acidithiobacillus thiooxidans
- Community Structure: Part of a "snottites" microbial community.
- Acid Production: Secretes sulphuric acid.
- Limestone Degradation: Degrades limestone.
- Gypsum Formation: Gypsum crystals form and acidic environment (pH 0-2) is created.
The Cave of Crystal Giants, Naica, Mexico
- A cave featured in the presentation with large gypsum crystals.
Industrial & Environmental Points
- Acid Mine Drainage: A. thiooxidans is linked with acid mine drainage where sulfur materials are utilized for metabolism.
- Environmental Impacts: Lowering of pH affects wildlife habitats.
Microbial Biodiversity Within Ice & Subsurface Lakes
- Knowledge is limited.
- Sampling, contamination, in addition to dormant microbes, cause problems in studying these populations.
- Metabolism rates are the lowest in these environments, in spite of the immense biotope size.
Summary
- Microbial adaptation to diverse extreme environments is exemplified by unique biological properties.
- Metabolic profiles in surface-water environments are largely aerobic, while deep-sea environments are mainly anaerobic
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Description
Test your knowledge on extremophiles and their unique adaptations to extreme environments. This quiz covers key concepts from the lecture, including the survival of microorganisms in high temperatures, the role of sulfur in Beggiatoa, and much more. Prepare to dive deep into the fascinating world of microbial life.