Microbial Culture and Media Quiz
21 Questions
0 Views

Choose a study mode

Play Quiz
Study Flashcards
Spaced Repetition
Chat to Lesson

Podcast

Play an AI-generated podcast conversation about this lesson

Questions and Answers

What is the purpose of selective media in microbiology?

  • To enhance the growth of all bacteria present
  • To recover and isolate specific bacteria from a mixed sample (correct)
  • To allow the identification of all organisms in a sample
  • To suppress the growth of specific bacteria while promoting others
  • Which of the following media is specifically selective for the isolation of mycobacteria?

  • Mannitol salt agar
  • Blood agar
  • CHROMagar
  • Lowestein-Jensen medium (correct)
  • What is the role of malachite green in Lowestein-Jensen medium?

  • To promote the growth of mycobacteria
  • To enhance lactose fermentation
  • To suppress gram-positive bacteria (correct)
  • To inhibit the growth of mycobacteria
  • What characteristic is often used to differentiate bacteria in differential media?

    <p>The coloration or pH change in the medium (B)</p> Signup and view all the answers

    What is the minimal inhibitory concentration (MIC) in antibiotic susceptibility testing?

    <p>The lowest concentration of an antibiotic that prevents visible bacterial growth (B)</p> Signup and view all the answers

    What is the primary purpose of microbial culture in the laboratory?

    <p>To multiply microbial organisms under controlled conditions. (A)</p> Signup and view all the answers

    Which type of culture media is specifically designed for the cultivation of fastidious bacteria?

    <p>Complex culture media (B)</p> Signup and view all the answers

    What characteristic is unique to agar as a solidifying agent in culture media?

    <p>It has solidifying properties from red-purple marine algae. (D)</p> Signup and view all the answers

    Which category of culture media is classified based on its ability to support the growth of certain bacteria while inhibiting others?

    <p>Selective media (B)</p> Signup and view all the answers

    What type of culture media contains only known and well-defined chemical constituents?

    <p>Synthetic culture media (C)</p> Signup and view all the answers

    What information can be obtained from in vitro cultures of pathogenic bacteria?

    <p>Morphology of the colonies and antimicrobial susceptibility. (C)</p> Signup and view all the answers

    What distinguishes complex culture media from synthetic culture media?

    <p>Complex media contain unknown amounts of nutrients. (C)</p> Signup and view all the answers

    Which culture media type is described as supporting the growth of microorganisms for further study?

    <p>Artificial growth media (D)</p> Signup and view all the answers

    Which characteristic of agar makes it suitable for use in microbiological applications?

    <p>Physiologically stable upon usage and storage (B)</p> Signup and view all the answers

    At what temperature does agar liquefy?

    <p>100°C (A)</p> Signup and view all the answers

    What is the primary purpose of a chemostat in microbiological research?

    <p>To maintain the Log growth phase of a bacterial population (C)</p> Signup and view all the answers

    What does the zone of inhibition indicate in a disk diffusion test?

    <p>The efficacy of the antimicrobial agent used (C)</p> Signup and view all the answers

    Which agar is recommended for antibiotic susceptibility testing?

    <p>Mueller-Hinton agar (D)</p> Signup and view all the answers

    What process is catalyzed by hemolysins observed in blood agar?

    <p>Lysis of erythrocytes (C)</p> Signup and view all the answers

    In a disk diffusion test, how much antimicrobial sample is typically loaded onto each disc?

    <p>10 µL (A)</p> Signup and view all the answers

    What is a primary function of enriched nonselective media like blood agar?

    <p>To test bacteria’s ability to lyse red blood cells (D)</p> Signup and view all the answers

    Flashcards

    Microbial Culture

    A technique used to multiply microbial organisms like bacteria and fungi by allowing them to reproduce in a controlled lab environment.

    Why is Microbial Culture Important?

    The study of microbial cultures helps in isolating and cultivating bacteria, understanding their morphology, and determining their susceptibility to antibiotics.

    Culture Media

    Materials that provide nutrients and conditions necessary for microorganisms to grow and multiply in the lab.

    Consistency of Culture Media

    Culture media classified by their physical form (liquid, solid, or semi-solid).

    Signup and view all the flashcards

    Complex Culture Media

    These media contain unknown amounts of nutrients, often including components like blood, serum, or yeast extract.

    Signup and view all the flashcards

    Synthetic Culture Media

    These media have a precisely defined chemical composition, with all ingredients known and measured.

    Signup and view all the flashcards

    Agar

    A type of solid culture media, made from a complex carbohydrate extracted from seaweed, used to solidify nutrient solutions.

    Signup and view all the flashcards

    Turbidity

    The appearance of a liquid culture media, characterized by cloudiness due to the growth of microorganisms.

    Signup and view all the flashcards

    Zone of inhibition

    The area around an antibiotic disk where bacteria cannot grow due to the antibiotic's effect. It's measured to assess the antibiotic's effectiveness.

    Signup and view all the flashcards

    Selective media

    A type of media that allows only specific bacteria to grow, while suppressing the growth of others. It's useful for isolating certain bacteria from a mixture.

    Signup and view all the flashcards

    Differential media

    A type of media that helps identify bacteria based on their unique characteristics. It usually contains substances that cause visible changes in the media, like color changes, based on the bacteria's activity.

    Signup and view all the flashcards

    Lowestein-Jensen medium

    A type of media specifically designed for growing Mycobacteria, which are bacteria that cause diseases like tuberculosis. It contains ingredients that both nourish the Mycobacteria and inhibit the growth of other bacteria.

    Signup and view all the flashcards

    CHROMagar

    A type of agar that contains a mixture of chromogenic substrates that are metabolized by different bacterial species, resulting in the production of distinct colors. This facilitates the identification of bacteria in a mixture.

    Signup and view all the flashcards

    Agar: What is it?

    A type of solidifying agent used in culture media, particularly for bacterial growth. It remains stable at room and body temperatures, non-toxic to bacteria, and resistant to degradation. It liquifies at 100°C and solidifies upon cooling to 50°C.

    Signup and view all the flashcards

    What is a bacterial colony?

    The visible growth of bacteria on a solid medium, often appearing as distinct isolated spots.

    Signup and view all the flashcards

    What is a bacterial growth curve?

    A graphical plot depicting the growth of a bacterial population over time. It showcases distinct phases, including lag, logarithmic (exponential), stationary, and death phases.

    Signup and view all the flashcards

    What is a chemostat?

    A device used to cultivate microorganisms under controlled conditions, allowing for continuous growth. It involves a constant supply of fresh medium and removal of waste products.

    Signup and view all the flashcards

    What is an antibiotic susceptibility test?

    The measurement of the susceptibility of bacteria to antimicrobial agents, often performed on agar media.

    Signup and view all the flashcards

    What is Mueller-Hinton agar?

    A type of agar-based media used for testing antibiotic susceptibility of bacteria. It allows clear zones of inhibition around antibiotic discs, indicating bacterial sensitivity.

    Signup and view all the flashcards

    What is the Kirby-Bauer test?

    A method for testing bacterial susceptibility to antibiotics. It involves placing antibiotic discs on an agar plate containing bacteria, measuring the clear zone of inhibition formed around the disc.

    Signup and view all the flashcards

    What is a hemolysis test?

    A test used for detecting the presence of bacterial enzymes, like hemolysins, that can lyse red blood cells. It utilizes blood agar plates.

    Signup and view all the flashcards

    Study Notes

    Bacterial Cultures

    • Microbial culture is a method to grow microorganisms (bacteria and fungi) in a lab.
    • In vitro cultures of bacteria allow for:
      • Isolation and cultivation of pathogenic bacteria
      • Morphology analysis of colonies and phenotypic characteristics for identification
      • Antibacterial susceptibility testing for choosing suitable antimicrobial agents
    • Microbial culture identification is crucial to determine the causative agent of a disease.

    Culture Media

    • Culture media are nutrient media, artificial growth mediums for microbial propagation.
    • Types of culture media are categorized by consistency (solid, semi-solid, liquid/broth).
    • Categorized by nutritional components:
      • Complex media (undefined): contain unknown nutrients, used for fastidious bacteria (requiring extra nutrients like blood, serum, yeast or meat extract).
      • Synthetic media (chemically defined): contain known and well-defined chemical constituents.
    • Categorized by function/property:
      • Selective media: promotes growth of certain bacteria while inhibiting others.
      • Differential media: allows differentiation of bacteria based on their metabolic activities (e.g. lactose fermentation).
      • Indicator media: show the presence of a specific substance in the medium.
      • Enriched media: support the growth of specific species that need extra nutrients.
      • Composite media: combination of properties.
      • Storage media: maintain viability of cultures.
      • Transport media: for safely carrying specimens for analysis.

    Liquid Culture Media (Nutrient Broth)

    • Nutrient Broth: liquid medium for nonfastidious microorganisms.
    • Can be supplemented with blood, serum, sugars, etc. for special purposes.
    • Typical formula (g/L): Beef Extract (1.0), Peptone (5.0), Yeast Extract (2.0), Sodium Chloride (5.0).
    • Final pH 6.8 ± 0.2 at 25°C.
    • Principle: components provide essential nutrients (amino acids, nitrogen, carbon, vitamins, minerals) and maintain osmotic balance for growth.

    Solid Culture Media (Agar)

    • Agar: unique solidifying agent from red-purple marine algae.
    • Properties:
      • Stable during sterilization
      • Physiologically stable upon usage and storage
      • Nontoxic to bacterial growth.
      • Liquifies at 100°C, solidifies at 50°C
    • Crucial for growing bacteria in agar plates for specific tests and observation.

    Solid Medium for Bacteria (Nutrient Agar)

    • Nutrient Agar ISO 6579: a non-selective medium for cultivating various microorganisms.
    • Typical formula (g/L): Meat Extract (3.0), Peptone (5.0), Agar (15.0).
    • Final pH 7.0 ± 0.2 at 25°C.
    • Principle: Components supply essential nutrients (amino acids, nitrogen, carbon, minerals, vitamins) for microbial growth. Agar is the solidifying agent.

    Microbial Growth Curve

    • Stages: Lag phase, Log phase (exponential growth), Stationary phase, and Decline phase.

    Continuous Culture Device (Chemostat)

    • Chemostat: maintains a constant Log phase of bacterial growth.
    • Flow control of fresh medium and outflow of spent medium.

    Bacterial Growth vs Oxygen Concentration

    • Obligate aerobes: require oxygen
    • Obligate anaerobes: cannot tolerate oxygen
    • Facultative anaerobes: can grow with or without oxygen
    • Aerotolerant anaerobes: tolerate oxygen but do not use it for growth.

    Bacterial Growth in Solid Media

    • Observed as colony formation.
    • Isolated colonies grow from single bacterial cells.
    • Colony characteristics (e.g., shape, elevation, margin) aid in bacterial identification.

    Bacterial Colony Morphology

    • Categorized by form, elevation, and margin shape

    Enriched Nonselective Media

    • Blood agar: contains 5% sheep/horse blood to test hemolysis.

    • Chocolate agar: modified blood agar via heating blood to a chocolate-brown colour.

    • Mueller-Hinton agar: recommended for antibiotic susceptibility testing.

    Disk Diffusion Test (Kirby-Bauer Test)

    • Antimicrobial susceptibility test performed on Mueller-Hinton agar.
    • Antibiotic discs are placed on the agar plate containing the microorganisms.
    • Inhibition zones are measured to determine the effectiveness of different antibiotics.

    Antibiotic Susceptibility Testing in Liquid Medium (MIC)

    • Serial 2-fold dilutions of antibiotics in Mueller-Hinton broth.
    • Inoculate with a known amount of microorganisms.
    • Determine the lowest antibiotic concentration that inhibits bacterial growth (MIC).

    Selective Media and Differential Media

    • Selective media: specifically designed to recover particular bacteria from a mixture.
    • Examples: Mannitol salt agar, Lowenstein-Jensen agar, CHROMagar.
    • Selective media can also be differential to visually identify and differentiate.

    Studying That Suits You

    Use AI to generate personalized quizzes and flashcards to suit your learning preferences.

    Quiz Team

    Related Documents

    Bacterial Cultures PDF

    Description

    Test your knowledge on microbial culture techniques and the various types of culture media used in laboratories. Learn about the importance of isolating pathogenic bacteria and the characteristics of different culture media. Enhance your understanding of microbial propagation methods and their applications in microbiology.

    More Like This

    Culture Media in Microbiology
    24 questions
    Microbial Culture Media Preparation
    24 questions
    Microbial Culture Media Overview
    24 questions
    Use Quizgecko on...
    Browser
    Browser