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Questions and Answers
Why are commercial food-canning protocols designed with a large margin of error?
Why are commercial food-canning protocols designed with a large margin of error?
A can of food is bulging. This is most likely due to the production of gases by which microbe?
A can of food is bulging. This is most likely due to the production of gases by which microbe?
Which of the following statements best describes why commercial sterilization might be considered a 'misnomer'?
Which of the following statements best describes why commercial sterilization might be considered a 'misnomer'?
What is the primary reason C. botulinum is a concern in canned foods?
What is the primary reason C. botulinum is a concern in canned foods?
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What is the purpose of heating low- and medium-acid foods to 121°C for a minimum of 2.52 minutes during commercial canning?
What is the purpose of heating low- and medium-acid foods to 121°C for a minimum of 2.52 minutes during commercial canning?
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Which of the following characteristics is essential for antiseptics, but not necessarily for disinfectants?
Which of the following characteristics is essential for antiseptics, but not necessarily for disinfectants?
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Why does typical disinfection not lead to sterilization?
Why does typical disinfection not lead to sterilization?
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Which category do surgical instruments belong to, based on the level of cleanliness required?
Which category do surgical instruments belong to, based on the level of cleanliness required?
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What is the primary purpose of degerming?
What is the primary purpose of degerming?
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Which of the following is an example of a semicritical item?
Which of the following is an example of a semicritical item?
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What is the main goal of sanitization in public health settings?
What is the main goal of sanitization in public health settings?
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Which of these methods primarily focuses on the mechanical removal, rather than killing, of microbes?
Which of these methods primarily focuses on the mechanical removal, rather than killing, of microbes?
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What is the key difference between antiseptics and degerming agents?
What is the key difference between antiseptics and degerming agents?
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Which of the following best describes fomites?
Which of the following best describes fomites?
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What is the primary function of an autoclave?
What is the primary function of an autoclave?
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Which of the following is the most accurate definition of sterilization?
Which of the following is the most accurate definition of sterilization?
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Why is aseptic technique crucial in medical and laboratory settings?
Why is aseptic technique crucial in medical and laboratory settings?
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What differentiates 'asepsis' from 'sepsis'?
What differentiates 'asepsis' from 'sepsis'?
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What is the defining characteristic of a 'sterile field'?
What is the defining characteristic of a 'sterile field'?
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What is the main goal of commercial sterilization in the food industry?
What is the main goal of commercial sterilization in the food industry?
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Why is vinegar considered a natural disinfectant?
Why is vinegar considered a natural disinfectant?
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What is the primary difference between a bactericidal and a bacteriostatic treatment?
What is the primary difference between a bactericidal and a bacteriostatic treatment?
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Which suffix indicates a method that inhibits the growth of fungi?
Which suffix indicates a method that inhibits the growth of fungi?
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Why might a -static treatment be preferred over a -cidal treatment in some situations?
Why might a -static treatment be preferred over a -cidal treatment in some situations?
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In what scenario would a '-static' treatment be sufficient to clear an infection?
In what scenario would a '-static' treatment be sufficient to clear an infection?
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What factor does not impact whether a treatment is -cidal or -static?
What factor does not impact whether a treatment is -cidal or -static?
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What describes the progress and effectiveness of a particular microbial control protocol?
What describes the progress and effectiveness of a particular microbial control protocol?
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Chemical disinfectants used to sanitize surfaces, in hospital rooms, primarily aim to achieve what outcome?
Chemical disinfectants used to sanitize surfaces, in hospital rooms, primarily aim to achieve what outcome?
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Why are -static chemicals safely impregnated into plastics?
Why are -static chemicals safely impregnated into plastics?
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Why is percentage killed a more useful measure than the absolute number of microbes killed when evaluating microbial death?
Why is percentage killed a more useful measure than the absolute number of microbes killed when evaluating microbial death?
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What is the decimal reduction time (DRT) or D-value?
What is the decimal reduction time (DRT) or D-value?
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If a microbial population is reduced by 99.9% after 3 D-values of exposure to a disinfectant, what percentage of the original population remains?
If a microbial population is reduced by 99.9% after 3 D-values of exposure to a disinfectant, what percentage of the original population remains?
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Why does it take longer to kill a high-population load of microbes compared to a low-population load using the same disinfection protocol?
Why does it take longer to kill a high-population load of microbes compared to a low-population load using the same disinfection protocol?
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What is the effect of organic debris, such as mud or feces, on the effectiveness of a disinfectant?
What is the effect of organic debris, such as mud or feces, on the effectiveness of a disinfectant?
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Which factor does NOT contribute to the effectiveness of a disinfecting agent or microbial control protocol?
Which factor does NOT contribute to the effectiveness of a disinfecting agent or microbial control protocol?
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What can be inferred about the relationship between disinfectant concentration and its effectiveness?
What can be inferred about the relationship between disinfectant concentration and its effectiveness?
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What would be effective for reaching the desired level of cleanliness when conditions limit contact between the agent and the targeted cells?
What would be effective for reaching the desired level of cleanliness when conditions limit contact between the agent and the targeted cells?
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What is a key characteristic of directional airflow in certain laboratories?
What is a key characteristic of directional airflow in certain laboratories?
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Which characteristic is associated with BSL-4 agents?
Which characteristic is associated with BSL-4 agents?
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Which of the following is a requirement when exiting a BSL-4 facility?
Which of the following is a requirement when exiting a BSL-4 facility?
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What critical safety feature is required when wearing a full-body protective suit in a BSL-4 laboratory?
What critical safety feature is required when wearing a full-body protective suit in a BSL-4 laboratory?
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Which of the following is a requirement for the location of a BSL-4 laboratory?
Which of the following is a requirement for the location of a BSL-4 laboratory?
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What is the purpose of using HEPA filters in BSL-4 laboratories?
What is the purpose of using HEPA filters in BSL-4 laboratories?
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Besides using a full-body suit with its own air supply in a BSL-4 environment, what additional measure can workers take while performing tasks?
Besides using a full-body suit with its own air supply in a BSL-4 environment, what additional measure can workers take while performing tasks?
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What is the primary criterion the CDC uses to classify infectious agents into biosafety levels?
What is the primary criterion the CDC uses to classify infectious agents into biosafety levels?
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Flashcards
Commercial Sterilization
Commercial Sterilization
A food sterilization protocol using heat to destroy pathogens while preserving quality.
C.botulinum
C.botulinum
A bacterium that produces toxins and can cause food poisoning.
Endospores
Endospores
Dormant, resistant forms of bacteria that can survive harsh conditions.
Quasi-sterilization
Quasi-sterilization
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Sterilization Temperature
Sterilization Temperature
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Chemical disinfectants
Chemical disinfectants
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Antiseptics
Antiseptics
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Antisepsis
Antisepsis
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Critical items
Critical items
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Semicritical items
Semicritical items
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Noncritical items
Noncritical items
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Degerming
Degerming
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Sanitization
Sanitization
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Percentage Killed
Percentage Killed
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Death Curves
Death Curves
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Decimal Reduction Time (DRT)
Decimal Reduction Time (DRT)
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Logarithmic Death
Logarithmic Death
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Exposure Time
Exposure Time
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Population Load
Population Load
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Concentration of Agent
Concentration of Agent
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Interfering Conditions
Interfering Conditions
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Fomites
Fomites
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Autoclave
Autoclave
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Sterilization
Sterilization
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Sterilants
Sterilants
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Aseptic Technique
Aseptic Technique
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Sepsis
Sepsis
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Sterile Field
Sterile Field
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Disinfection
Disinfection
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BSL-4 Laboratories
BSL-4 Laboratories
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Directional Airflow
Directional Airflow
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BSL-4 Agents
BSL-4 Agents
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Full-body Protective Suit
Full-body Protective Suit
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HEPA Filters
HEPA Filters
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Laboratory Decontamination
Laboratory Decontamination
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Positive Air Pressure
Positive Air Pressure
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Biosafety Levels (BSLs)
Biosafety Levels (BSLs)
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Disinfectant
Disinfectant
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-cide
-cide
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Bactericide
Bactericide
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Viricide
Viricide
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Fungicide
Fungicide
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-static
-static
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Bacteriostatic
Bacteriostatic
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Microbial death curve
Microbial death curve
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Study Notes
Microbial Control
- Microbial growth and abundance must be controlled to prevent human disease
- Fomites are inanimate objects that harbor microbes and aid in disease transmission
- Factors influencing cleanliness protocols include application use and pathogen resistance
- Food preservation methods often require extreme temperatures/pressures to eliminate endospores
Laboratory Biological Safety Levels (BSLs)
- Risks associated with pathogens determine cleanliness levels
- Centers for Disease Control and Prevention (CDC) and National Institutes of Health (NIH) have established BSL classifications
- BSL levels are determined by infectivity, transmissibility, severity, and type of work being done
- BSL-1 agents pose minimal risk to healthy adults and include nonpathogenic E. coli and Bacillus subtilis
- BSL-1 uses standard aseptic techniques
- BSL-2 agents pose moderate risk and are frequently found in a geographical area; examples include Staphylococcus aureus and Salmonella
- BSL-2 requires restricted access, specific PPE (including face shields), biological safety cabinets, and other equipment
- BSL-3 agents are potential lethal infectious agents by inhalation and often include indigenous or exotic pathogens; an example includes Mycobacterium tuberculosis
- BSL-3 requires restricted access, medical surveillance, respirators, and directional airflow
- BSL-4 agents are the most dangerous and often fatal, which are exotic and easily transmitted; includes Ebola virus or Marburg virus
- BSL-4 requires full body protective suits with HEPA filtered air, separate buildings or isolated portions, and decontamination systems
Sterilization
- Sterilization aims to completely remove all vegetative cells, endospores, and viruses
- Physical methods include high heat, pressure, or filtration; chemical methods include sterilants
- Critical items require sterilization (eg, surgical instruments) due to their use inside the body
- Semi-critical items need high levels of disinfection
- Non-critical items need cleanliness but not high levels of disinfection
- Techniques like handwashing are forms of degerming
Other Methods of Control
- Disinfection is used on surfaces to reduce microbial load, and use a variety of chemicals
- Disinfectants are used on non-living tissue/objects
- Antiseptics are used in environments involving living tissue
- Sanitization reduces microbial loads to safe levels for public health
- Aseptic technique avoids microbial contamination.
Key Terms
- Fomites: inanimate objects harbouring microbes and aiding disease transmission
- Autoclave: device for moist-heat sterilization using pressure and steam
- Sterilization: complete removal of vegetative cells, endospores, and viruses
- Sterilants: strong chemicals that kill all microbes and viruses
- Aseptic technique: method preventing microbial contamination, including sterile locations or tissues
- Sepsis: systemic response to infection causing high fever and edema
- Sterile field: an area free of all vegetative microbes, endospores, and viruses
- Disinfection: removing pathogens from non-living objects.
- Disinfectants: antimicrobial chemicals used for disinfection that may be toxic to tissues
- Antiseptics: antimicrobial chemicals safely used on living tissue
- Antisepsis: protocols to remove pathogens from living tissue
- Critical items: objects penetrating sterile tissues; require sterilization
- Semi-critical items: objects contacting mucous membranes; require high disinfection
- Non-critical items: objects touching intact skin; require cleanliness but not high-level disinfection
- Degerming: reducing microbial numbers using mild chemicals and gentle scrubbing (example: handwashing)
- Sanitization: reducing microbial level to safe levels for public health, e.g. in dishwashing
- Bactericides: chemical/physical agents killing bacteria
- Virucides: chemical/physical agents killing viruses
- Fungicides: chemical/physical agents killing fungi
- Bacteriostatic: agents inhibiting bacterial growth
- Fungistatic: agents inhibiting fungal growth
- Decimal reduction time (DRT) or D-value: time it takes to kill 90% of a microbial population
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Description
This quiz covers key concepts in microbial control, including the importance of controlling microbial growth to prevent disease. It also explores the classification of Laboratory Biological Safety Levels (BSLs) and the associated risks of various pathogens.