Microbial Control and BSLs Overview
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Questions and Answers

Why are commercial food-canning protocols designed with a large margin of error?

  • To reduce the cost and energy consumption of sterilization processes, making canned foods more affordable.
  • To ensure the survival of nonpathogenic organisms that contribute to the food's flavor and texture.
  • To achieve complete sterilization, eliminating all microorganisms, including those that are beneficial.
  • To account for the potential contamination of food with an impossibly large population of _C. botulinum_ endospores. (correct)
  • A can of food is bulging. This is most likely due to the production of gases by which microbe?

  • Salmonella
  • Listeria
  • C. botulinum (correct)
  • E. coli
  • Which of the following statements best describes why commercial sterilization might be considered a 'misnomer'?

  • It uses extremely high temperatures that destroy the nutritional value and flavor of the food.
  • It primarily focuses on eliminating spoilage and foodborne disease-causing pathogens, but allows some nonpathogenic organisms to survive. (correct)
  • It relies solely on chemical disinfectants, which are not as effective as physical sterilization methods.
  • It eliminates all forms of microbial life, including viruses and prions.
  • What is the primary reason C. botulinum is a concern in canned foods?

    <p>It produces toxins in anaerobic environments, such as improperly canned foods. (D)</p> Signup and view all the answers

    What is the purpose of heating low- and medium-acid foods to 121°C for a minimum of 2.52 minutes during commercial canning?

    <p>To reduce a population of $10^{12}$ <em>C. botulinum</em> endospores per can down to 1 endospore. (B)</p> Signup and view all the answers

    Which of the following characteristics is essential for antiseptics, but not necessarily for disinfectants?

    <p>Safe use on living tissues (B)</p> Signup and view all the answers

    Why does typical disinfection not lead to sterilization?

    <p>Endospores can survive the disinfection process. (D)</p> Signup and view all the answers

    Which category do surgical instruments belong to, based on the level of cleanliness required?

    <p>Critical (B)</p> Signup and view all the answers

    What is the primary purpose of degerming?

    <p>To reduce the number of microbes on living tissue (C)</p> Signup and view all the answers

    Which of the following is an example of a semicritical item?

    <p>Gastrointestinal endoscopes (C)</p> Signup and view all the answers

    What is the main goal of sanitization in public health settings?

    <p>Reduction of microbes to a safe level (A)</p> Signup and view all the answers

    Which of these methods primarily focuses on the mechanical removal, rather than killing, of microbes?

    <p>Handwashing with soap (C)</p> Signup and view all the answers

    What is the key difference between antiseptics and degerming agents?

    <p>Antiseptics aim to kill or inhibit microbes, while degerming agents primarily remove them. (A)</p> Signup and view all the answers

    Which of the following best describes fomites?

    <p>Inanimate objects that may harbor microbes and aid in disease transmission. (C)</p> Signup and view all the answers

    What is the primary function of an autoclave?

    <p>To sterilize materials using moist-heat under pressure. (C)</p> Signup and view all the answers

    Which of the following is the most accurate definition of sterilization?

    <p>The process that completely removes all vegetative cells, endospores, and viruses from an item. (B)</p> Signup and view all the answers

    Why is aseptic technique crucial in medical and laboratory settings?

    <p>To prevent microbial contamination of sterile objects, locations, or tissues. (D)</p> Signup and view all the answers

    What differentiates 'asepsis' from 'sepsis'?

    <p>Asepsis refers to a sterile state while sepsis refers to a systemic inflammatory response to an infection. (D)</p> Signup and view all the answers

    What is the defining characteristic of a 'sterile field'?

    <p>A specified area that is free of all vegetative microbes, endospores, and viruses. (B)</p> Signup and view all the answers

    What is the main goal of commercial sterilization in the food industry?

    <p>To destroy vegetative cells and endospores of common foodborne pathogens while preserving food quality. (B)</p> Signup and view all the answers

    Why is vinegar considered a natural disinfectant?

    <p>Its acidity kills most microbes. (B)</p> Signup and view all the answers

    What is the primary difference between a bactericidal and a bacteriostatic treatment?

    <p>Bactericidal treatments kill bacteria directly, while bacteriostatic treatments only inhibit their growth. (B)</p> Signup and view all the answers

    Which suffix indicates a method that inhibits the growth of fungi?

    <p>-static (A)</p> Signup and view all the answers

    Why might a -static treatment be preferred over a -cidal treatment in some situations?

    <p>-Static treatments are often less harmful to humans and can better preserve the treated item. (B)</p> Signup and view all the answers

    In what scenario would a '-static' treatment be sufficient to clear an infection?

    <p>In an otherwise healthy individual, preventing the pathogen from multiplying so the immune system can clear the infection. (B)</p> Signup and view all the answers

    What factor does not impact whether a treatment is -cidal or -static?

    <p>The size of the area being treated. (B)</p> Signup and view all the answers

    What describes the progress and effectiveness of a particular microbial control protocol?

    <p>Microbial death curve (D)</p> Signup and view all the answers

    Chemical disinfectants used to sanitize surfaces, in hospital rooms, primarily aim to achieve what outcome?

    <p>Prevent disease transmission between patients. (D)</p> Signup and view all the answers

    Why are -static chemicals safely impregnated into plastics?

    <p>To prevent the growth of microbes on these surfaces. (B)</p> Signup and view all the answers

    Why is percentage killed a more useful measure than the absolute number of microbes killed when evaluating microbial death?

    <p>Because the rate of killing remains constant regardless of population size. (A)</p> Signup and view all the answers

    What is the decimal reduction time (DRT) or D-value?

    <p>The time required to decrease the number of organisms by one order of magnitude (90%). (A)</p> Signup and view all the answers

    If a microbial population is reduced by 99.9% after 3 D-values of exposure to a disinfectant, what percentage of the original population remains?

    <p>0.1% of the original population remains. (D)</p> Signup and view all the answers

    Why does it take longer to kill a high-population load of microbes compared to a low-population load using the same disinfection protocol?

    <p>Microbial death is logarithmic, requiring more time to achieve the same percentage kill in a larger population. (B)</p> Signup and view all the answers

    What is the effect of organic debris, such as mud or feces, on the effectiveness of a disinfectant?

    <p>Organic debris limits contact between the disinfectant and the target cells, reducing effectiveness. (A)</p> Signup and view all the answers

    Which factor does NOT contribute to the effectiveness of a disinfecting agent or microbial control protocol?

    <p>The size of the container the agent is in (D)</p> Signup and view all the answers

    What can be inferred about the relationship between disinfectant concentration and its effectiveness?

    <p>Higher disinfectant concentration is directly related to disinfectant effectiveness. (D)</p> Signup and view all the answers

    What would be effective for reaching the desired level of cleanliness when conditions limit contact between the agent and the targeted cells?

    <p>Increase the cleaning time (B)</p> Signup and view all the answers

    What is a key characteristic of directional airflow in certain laboratories?

    <p>Air is pulled from clean areas to potentially contaminated areas. (D)</p> Signup and view all the answers

    Which characteristic is associated with BSL-4 agents?

    <p>They are often fatal and easily transmitted by inhalation. (C)</p> Signup and view all the answers

    Which of the following is a requirement when exiting a BSL-4 facility?

    <p>Decontaminating all material, changing clothing, and showering are necessary. (A)</p> Signup and view all the answers

    What critical safety feature is required when wearing a full-body protective suit in a BSL-4 laboratory?

    <p>The air pressure inside the suit must be higher than that outside the suit. (B)</p> Signup and view all the answers

    Which of the following is a requirement for the location of a BSL-4 laboratory?

    <p>It must be located in a separate building or isolated portion of a building. (B)</p> Signup and view all the answers

    What is the purpose of using HEPA filters in BSL-4 laboratories?

    <p>To filter out particulate matter, including infectious agents, from the air supply and exhaust. (A)</p> Signup and view all the answers

    Besides using a full-body suit with its own air supply in a BSL-4 environment, what additional measure can workers take while performing tasks?

    <p>Conduct all work within a biological safety cabinet with HEPA-filtered air. (C)</p> Signup and view all the answers

    What is the primary criterion the CDC uses to classify infectious agents into biosafety levels?

    <p>Potential risk to laboratory personnel and the community. (C)</p> Signup and view all the answers

    Flashcards

    Commercial Sterilization

    A food sterilization protocol using heat to destroy pathogens while preserving quality.

    C.botulinum

    A bacterium that produces toxins and can cause food poisoning.

    Endospores

    Dormant, resistant forms of bacteria that can survive harsh conditions.

    Quasi-sterilization

    A more accurate term for commercial sterilization, as not all microbes are eliminated.

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    Sterilization Temperature

    Foods are heated to 121°C for a minimum of 2.52 minutes to kill pathogens.

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    Chemical disinfectants

    Substances used to clean nonliving surfaces by killing most microorganisms but not spores.

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    Antiseptics

    Antimicrobial chemicals safe for living tissues, effective against microorganisms.

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    Antisepsis

    The process of applying antiseptics to living tissues to reduce microbial presence.

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    Critical items

    Instruments needing to be sterile as they enter sterile body areas.

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    Semicritical items

    Items that contact mucous membranes but do not penetrate tissues; need high-level disinfection.

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    Noncritical items

    Items that only contact intact skin; require cleaning but not high disinfection.

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    Degerming

    The act of reducing microbial numbers on living tissue, often via scrubbing with soap.

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    Sanitization

    Cleansing fomites to remove enough microbes for public health safety.

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    Percentage Killed

    The ratio of microbes killed relative to the total population size, more informative than absolute numbers.

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    Death Curves

    Graphs that show the logarithmic reduction of microorganisms over time, often using semilog plots.

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    Decimal Reduction Time (DRT)

    The time required to kill 90% of a microbial population through a specific protocol.

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    Logarithmic Death

    The pattern where the rate of microbial death is proportional to the number of survivors, not the total.

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    Exposure Time

    The duration a disinfecting agent is in contact with microbes, affecting kill rates.

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    Population Load

    The initial number of microorganisms present that influences effectiveness of microbicidal protocols.

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    Concentration of Agent

    The amount of disinfecting agent used; higher concentrations kill microbes more effectively and quickly.

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    Interfering Conditions

    Factors like bodily fluids or debris that can hinder the effectiveness of disinfectants and lengthen treatment time.

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    Fomites

    Inanimate items that may harbor microbes and aid disease transmission.

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    Autoclave

    Device for moist-heat sterilization using pressure to steam.

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    Sterilization

    Protocol that completely removes all microbes from an item.

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    Sterilants

    Strong chemicals that effectively kill all microbes on inanimate items.

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    Aseptic Technique

    Method designed to prevent microbial contamination of sterile objects.

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    Sepsis

    Systemic inflammatory response to infection that may cause severe illness.

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    Sterile Field

    Area that is free of all vegetative microbes, endospores, and viruses.

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    Disinfection

    Process that inactivates most microbes on fomite surfaces using chemicals or heat.

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    BSL-4 Laboratories

    Contain the most dangerous pathogens requiring maximum precautions.

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    Directional Airflow

    Clean air is pulled from clean to contaminated areas.

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    BSL-4 Agents

    Highly dangerous microbes with no treatments or vaccines.

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    Full-body Protective Suit

    A suit worn in BSL-4 labs that supplies its own air.

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    HEPA Filters

    Filters that trap particulates in biological safety cabinets.

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    Laboratory Decontamination

    Process of cleaning materials and equipment before leaving a lab.

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    Positive Air Pressure

    Pressure in protective suits is higher than outside to prevent contamination.

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    Biosafety Levels (BSLs)

    Classification system for infectious agents based on risk level.

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    Disinfectant

    A chemical used to sanitize surfaces and prevent disease.

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    -cide

    Suffix indicating methods that kill a specific microorganism.

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    Bactericide

    A substance that kills bacteria.

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    Viricide

    A substance that kills or inactivates viruses.

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    Fungicide

    A substance that kills fungi.

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    -static

    Suffix indicating methods that inhibit the growth of microorganisms.

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    Bacteriostatic

    A treatment that inhibits bacterial growth without killing them.

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    Microbial death curve

    A graphical representation showing the effectiveness of microbial control over time.

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    Study Notes

    Microbial Control

    • Microbial growth and abundance must be controlled to prevent human disease
    • Fomites are inanimate objects that harbor microbes and aid in disease transmission
    • Factors influencing cleanliness protocols include application use and pathogen resistance
    • Food preservation methods often require extreme temperatures/pressures to eliminate endospores

    Laboratory Biological Safety Levels (BSLs)

    • Risks associated with pathogens determine cleanliness levels
    • Centers for Disease Control and Prevention (CDC) and National Institutes of Health (NIH) have established BSL classifications
    • BSL levels are determined by infectivity, transmissibility, severity, and type of work being done
    • BSL-1 agents pose minimal risk to healthy adults and include nonpathogenic E. coli and Bacillus subtilis
    • BSL-1 uses standard aseptic techniques
    • BSL-2 agents pose moderate risk and are frequently found in a geographical area; examples include Staphylococcus aureus and Salmonella
    • BSL-2 requires restricted access, specific PPE (including face shields), biological safety cabinets, and other equipment
    • BSL-3 agents are potential lethal infectious agents by inhalation and often include indigenous or exotic pathogens; an example includes Mycobacterium tuberculosis
    • BSL-3 requires restricted access, medical surveillance, respirators, and directional airflow
    • BSL-4 agents are the most dangerous and often fatal, which are exotic and easily transmitted; includes Ebola virus or Marburg virus
    • BSL-4 requires full body protective suits with HEPA filtered air, separate buildings or isolated portions, and decontamination systems

    Sterilization

    • Sterilization aims to completely remove all vegetative cells, endospores, and viruses
    • Physical methods include high heat, pressure, or filtration; chemical methods include sterilants
    • Critical items require sterilization (eg, surgical instruments) due to their use inside the body
    • Semi-critical items need high levels of disinfection
    • Non-critical items need cleanliness but not high levels of disinfection
    • Techniques like handwashing are forms of degerming

    Other Methods of Control

    • Disinfection is used on surfaces to reduce microbial load, and use a variety of chemicals
    • Disinfectants are used on non-living tissue/objects
    • Antiseptics are used in environments involving living tissue
    • Sanitization reduces microbial loads to safe levels for public health
    • Aseptic technique avoids microbial contamination.

    Key Terms

    • Fomites: inanimate objects harbouring microbes and aiding disease transmission
    • Autoclave: device for moist-heat sterilization using pressure and steam
    • Sterilization: complete removal of vegetative cells, endospores, and viruses
    • Sterilants: strong chemicals that kill all microbes and viruses
    • Aseptic technique: method preventing microbial contamination, including sterile locations or tissues
    • Sepsis: systemic response to infection causing high fever and edema
    • Sterile field: an area free of all vegetative microbes, endospores, and viruses
    • Disinfection: removing pathogens from non-living objects.
    • Disinfectants: antimicrobial chemicals used for disinfection that may be toxic to tissues
    • Antiseptics: antimicrobial chemicals safely used on living tissue
    • Antisepsis: protocols to remove pathogens from living tissue
    • Critical items: objects penetrating sterile tissues; require sterilization
    • Semi-critical items: objects contacting mucous membranes; require high disinfection
    • Non-critical items: objects touching intact skin; require cleanliness but not high-level disinfection
    • Degerming: reducing microbial numbers using mild chemicals and gentle scrubbing (example: handwashing)
    • Sanitization: reducing microbial level to safe levels for public health, e.g. in dishwashing
    • Bactericides: chemical/physical agents killing bacteria
    • Virucides: chemical/physical agents killing viruses
    • Fungicides: chemical/physical agents killing fungi
    • Bacteriostatic: agents inhibiting bacterial growth
    • Fungistatic: agents inhibiting fungal growth
    • Decimal reduction time (DRT) or D-value: time it takes to kill 90% of a microbial population

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    Description

    This quiz covers key concepts in microbial control, including the importance of controlling microbial growth to prevent disease. It also explores the classification of Laboratory Biological Safety Levels (BSLs) and the associated risks of various pathogens.

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