MICR 290 Course Overview Quiz

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Questions and Answers

What is the main focus of the MICR 290 course?

Developing new vaccines

Understanding viral infections

Studying human anatomy

Controlling bacterial growth (correct)

Which method is NOT mentioned as a way to control bacterial growth?

Antisepsis

Radiation therapy (correct)

Disinfection

Sanitization

What is the purpose of testing antibiotic susceptibility?

To measure bacterial growth rates

To discover new antibiotic compounds

To aid in vaccine development

To determine the effectiveness of an antibiotic against specific bacteria (correct)

Which section of the module focuses on antibiotic discovery?

Section 06 Signup and view all the answers

Why is the control of bacterial growth considered critical?

Bacteria can cause infections that impact health Signup and view all the answers

Which option describes antisepsis?

The destruction of pathogens on living tissue Signup and view all the answers

What could be a consequence of distributing the Module Companion Guide illegally?

Sanctions for academic integrity violation Signup and view all the answers

Which of the following is not a section title mentioned in the module contents?

Alternative Antibiotics Signup and view all the answers

What is the primary mechanism by which soap removes bacteria from the skin?

Mechanical removal through the disruption of oily films Signup and view all the answers

Which of the following is NOT a characteristic of antibiotics?

Used primarily for treating viral infections Signup and view all the answers

Which chemical method is primarily used to disrupt lipid membranes?

Alcohol Signup and view all the answers

What is a common method used to determine the efficacy of chemical agents at killing bacteria?

Disk diffusion test Signup and view all the answers

Which of the following chemicals reacts with DNA to modify nitrogenous bases?

Bleach Signup and view all the answers

What happens to proteins when they are denatured by chemical agents?

Their structure is altered, leading to loss of function Signup and view all the answers

Which of the following statements about disinfectants and antiseptics is correct?

Disinfectants usually have a broader application than antiseptics. Signup and view all the answers

Which class of chemicals interrupts protein functions through hydrophobic interactions?

Alcohols Signup and view all the answers

What happens to most bacteria at low temperatures below 8 °C?

Their metabolism slows down. Signup and view all the answers

Which of the following is a characteristic of using high temperatures to control bacterial growth?

It can kill bacteria effectively. Signup and view all the answers

What is the primary effect of refrigeration on bacteria in food?

It inhibits bacterial growth. Signup and view all the answers

Which statement about bacterial survival at low temperatures is true?

Some bacteria can grow slowly at low temperatures. Signup and view all the answers

How do physical methods of bacterial control differ from chemical methods?

Physical methods utilize agents like temperature and radiation. Signup and view all the answers

What is a common misconception about low temperatures and bacterial reproduction?

They slow down the metabolism but do not kill most bacteria. Signup and view all the answers

What is the primary purpose of using high temperatures in bacterial control methods?

To kill bacteria directly. Signup and view all the answers

Which of the following statements about temperature control methods is accurate?

Low temperatures primarily inhibit bacterial growth without killing. Signup and view all the answers

What happens if the annealing temperature is set too high during PCR?

Primers are unable to bind to the template. Signup and view all the answers

Why is it important to determine the optimal annealing temperature on a case-by-case basis?

It ensures primers can bind to the template DNA. Signup and view all the answers

What is the typical extension temperature for DNA polymerase during PCR?

68 - 72 °C Signup and view all the answers

What role do the primers play during the PCR process?

They bind to the template DNA. Signup and view all the answers

Why are thermostable DNA polymerases used in PCR?

They can function at high temperatures without denaturing. Signup and view all the answers

What is a potential consequence of setting the annealing temperature too low?

Primers may bind to unintended templates. Signup and view all the answers

What is the significance of the complementary nucleotide sequences of the primers?

They allow for specific binding to the template DNA. Signup and view all the answers

Which of the following correctly describes Taq polymerase?

It is derived from a thermophilic bacterium. Signup and view all the answers

What is the primary reason for increasing the ionic strength of a buffer in protein purification?

To stabilize protein interactions without altering pH Signup and view all the answers

What is a potential consequence of washing a cation exchange column with Buffer B directly?

All bound proteins will elute, contaminating the desired protein Signup and view all the answers

Which of the following methods can be used to create a buffer gradient in purification processes?

Employing an FPLC with separate pumps for each buffer Signup and view all the answers

In an ion exchange chromatography setup, which proteins elute first during a gradual increase in ionic strength?

Proteins that are less positively charged Signup and view all the answers

What are the two types of gradient washes used in cation exchange chromatography?

Stepwise and linear wash Signup and view all the answers

Why might one use nickel affinity chromatography instead of ion exchange chromatography?

It specifically targets histidine-tagged proteins Signup and view all the answers

During cation exchange chromatography, which characteristic determines the order in which proteins are eluted?

The charge of the proteins in relation to the buffer Signup and view all the answers

Which of the following best describes a linear gradient in buffer washing?

Percentage of Buffer B increases consistently over time Signup and view all the answers

What effect does a high ionic strength buffer have on proteins during ion exchange chromatography?

It allows salt ions to outcompete proteins for binding to the resin. Signup and view all the answers

Which buffer condition is best for enhancing the positive charge of a recombinant protein in cation exchange chromatography?

Low pH with low salt concentration Signup and view all the answers

How can you elute the recombinant protein from a cation exchange column?

Increase the salt concentration of the buffer. Signup and view all the answers

What happens to neutral or negatively charged proteins during the washing phase of cation exchange chromatography?

They elute quickly from the column. Signup and view all the answers

What is the main purpose of adjusting the buffer during the elution of proteins in cation exchange chromatography?

To separate the recombinant protein from other charged proteins. Signup and view all the answers

What role do salt ions play in the context of ion exchange chromatography?

They compete with proteins for resin binding sites. Signup and view all the answers

What would be the impact of using a buffer with low ionic strength in cation exchange chromatography?

It allows proteins to bind more effectively to the resin. Signup and view all the answers

In ion exchange chromatography, what is the consequence of using a high pH buffer?

It enhances the binding of negatively charged proteins. Signup and view all the answers

Study Notes

Information on the Module Companion Guide

This guide complements the online course slides.
There may be discrepancies between the guide and the online module. If so, refer to the online module.
Do not distribute the guide to students who are not enrolled in the course. Doing so violates the academic integrity policy.

Information on Protecting the Integrity of the Course

Do not share the Module Companion Guide with students who are not enrolled in the course; this is a violation of academic integrity.
Students caught violating the Academic Integrity Policy at Queen's University can face sanctions.

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Description

Test your knowledge on the key concepts of the MICR 290 course. This quiz covers topics such as bacterial growth control, antibiotic susceptibility testing, and antisepsis. Challenge yourself to see how well you understand the critical aspects of microbiology and the mechanisms involved in combating bacterial infections.