Methods for Analyzing Membrane Proteins

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Questions and Answers

What method is used for visualizing the structure inside chloroplasts?

  • Optical microscope
  • Ultra centrifugation separation technique
  • Spectroscopic methods
  • Confocal fluorescence imaging (correct)

What is the main limitation of optical microscopy in visualizing membranes containing photosynthetic complexes?

  • Inability to probe membrane protein functions
  • Inability to differentiate between different membrane proteins
  • Resolution limit of 300 nanometers (correct)
  • Limited dynamic range

Which method enables probing the dynamics of membrane proteins in vivo?

  • Confocal fluorescence imaging
  • Ultra centrifugation separation technique
  • Spectroscopic methods (correct)
  • Visual methods

What is the primary purpose of ultra centrifugation separation technique mentioned in the lecture?

<p>Isolation of membrane proteins from the photosynthetic membrane (A)</p> Signup and view all the answers

What is the size range of membranes containing photosynthetic complexes mentioned in the lecture?

<p>Smaller than one micrometer, about 500 nanometers and less (B)</p> Signup and view all the answers

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