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Metagenomics Lecture

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Questions and Answers

What is the primary focus of targeted metagenomics?

To study the entire taxonomic structure of microbial communities
To study the composition of microbial communities using marker genes (correct)
To assess the functional potential of microbial communities
To Sequence the entire genomic DNA of microbial communities

What is the main difference between targeted metagenomics and shotgun metagenomics?

The focus of the study, either on specific genes or the entire genome (correct)
The type of DNA extraction method used
The environment being studied, either soil or ocean
The type of sequencing technology used

What is the name of the database that stores genomic DNA sequences obtained from metagenomics studies?

National Center for Biotechnology Information (NCBI)
Sequence Read Archive (SRA) (correct)
MetaGeneBank
GenBank

What is the advantage of metagenomics over traditional cultivation-based methods?

It allows for the study of uncultivated microorganisms (A) Signup and view all the answers

What is the first step in the metagenomics approach?

Selecting an interesting environment (D) Signup and view all the answers

What is the term for the genetic material obtained directly from environmental samples?

Metagenome (B) Signup and view all the answers

What is the name of the next-generation sequencing technology used in metagenomics?

High-throughput sequencing (D) Signup and view all the answers

What is the purpose of constructing a genomic library in metagenomics?

To prepare genomic DNA for sequencing (D) Signup and view all the answers

What is an example of an environment where metagenomics can be applied?

Thermophilic bioreactors (C) Signup and view all the answers

What is the main goal of shotgun metagenomics?

To achieve a wide coverage of genomic DNA sequences (B) Signup and view all the answers

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