Meselson and Stahl Experiments on DNA Replication

<p>One low-density and one intermediate-density strand</p> Signup and view all the answers

<p>DNA is the genetic material</p> Signup and view all the answers

<p>Radioactive sulphur (35S)</p> Signup and view all the answers

<p>DNA</p> Signup and view all the answers

<p>Semiconservative replication gives two duplex DNA molecules, each of which contains one old strand and one new strand</p> Signup and view all the answers

<p>Nucleic acid biochemistry</p> Signup and view all the answers

<p>The biochemical processes used in DNA replication</p> Signup and view all the answers

<p>It has a copying mechanism</p> Signup and view all the answers

<p>Chemical reasons</p> Signup and view all the answers

<p>To proofread the newly synthesized strand</p> Signup and view all the answers

<p>By exonuclease activity of the polymerase complex</p> Signup and view all the answers

<p>To introduce a break in one strand and pass the other strand through</p> Signup and view all the answers

<p>Ligase enzyme</p> Signup and view all the answers

<p>It is replaced by another polymerase (Pol I)</p> Signup and view all the answers

<p>To alleviate topological problems caused by over-winding of the double helix</p> Signup and view all the answers

<p>The bacterial pellet was non-radioactive when infected by the 35S–viruses.</p> Signup and view all the answers

<p>Proteins</p> Signup and view all the answers

<p>It gives rise to two duplex DNA molecules, each containing one old strand and one new strand.</p> Signup and view all the answers

<p>To unwind the DNA double helix.</p> Signup and view all the answers

<p>To initiate DNA replication by providing a starting point for DNA polymerase</p> Signup and view all the answers

<p>To replicate both strands of DNA simultaneously</p> Signup and view all the answers

<p>To alleviate topological problems by resolving over-winding of the double helix</p> Signup and view all the answers

<p>DNA polymerase I</p> Signup and view all the answers

<p>Over-winding of the double helix</p> Signup and view all the answers

<p>Ligase</p> Signup and view all the answers

<p>It signals the completion of DNA replication once the replicons meet at a specific point</p> Signup and view all the answers

<p>Eukaryotic replication forks move at around 50 base pairs per second due to the complexity of eukaryotic DNA structures</p> Signup and view all the answers

<p>They are responsible for unlinking the interlinked daughter DNA molecules</p> Signup and view all the answers

<p>They are segregated and await cell division, where each cell will receive a DNA molecule</p> Signup and view all the answers

<p>They open up a local AT-rich region of the DNA via torsional stress.</p> Signup and view all the answers

<p>To prevent the strands from re-annealing and protect the DNA from attack by free radicals and nuclease enzymes.</p> Signup and view all the answers

<p>Carrying out synthesis of the new strand in the 5’ to 3’ direction.</p> Signup and view all the answers

<p>Synthesizing the RNA primer on the leading strand.</p> Signup and view all the answers

<p>Alleviating torsional stress on the DNA molecule by causing temporary nicks in the DNA backbone.</p> Signup and view all the answers

<p>Breaking base-pairs (hydrogen bonds) at the replication fork.</p> Signup and view all the answers

<p>It has not escaped our notice that the specific pairing we have postulated immediately suggests a possible copying mechanism for the genetic material.</p> Signup and view all the answers

<p>The biochemical processes used in the replication of DNA in prokaryotes and eukaryotes, the experiments used to understand DNA replication, and how DNA replication is controlled as part of the cell cycle.</p> Signup and view all the answers

<p>DNA is a helix due to its specific pairing, which suggests a possible copying mechanism for the genetic material.</p> Signup and view all the answers

<p>The distinguishing factor is that eukaryotic DNA replication forks have multiple origins of replication, while prokaryotic DNA replication forks have a single origin of replication.</p> Signup and view all the answers

<p>α, ε, θ</p> Signup and view all the answers

<p>τ</p> Signup and view all the answers

<p>Two homodimers of the β subunit; provides the ring structure needed for processivity</p> Signup and view all the answers

<p>γ, δ, δ', χ, ψ</p> Signup and view all the answers

<p>Half low-density and half intermediate-density</p> Signup and view all the answers

<p>Origin of replication; gives rise to two replication forks</p> Signup and view all the answers

<p>Replication fork; DNA synthesis occurs at this point</p> Signup and view all the answers

<p>Semiconservative replication model</p> Signup and view all the answers

<p>11 bp</p> Signup and view all the answers

<p>50,000 - 100,000</p> Signup and view all the answers

<p>Random initiation</p> Signup and view all the answers

<p>To fully and controllably replicate DNA prior to cell division</p> Signup and view all the answers